Anticancer effects of the combined Thai noni juice ethanolic extracts and 5-fluorouracil against cholangiocarcinoma cells in vitro and in vivo

Abstract Application of 5-fluorouracil (5-FU) in cholangiocarcinoma (CCA) is limited by adverse side effects and chemoresistance. Therefore, the combination therapy of 5-FU with other substances, especially natural products may provide a new strategy for CCA treatment. The aim of this study was to e...

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Autores principales: Jeerati Prompipak, Thanaset Senawong, Banchob Sripa, Albert J. Ketterman, Suppawit Utaiwat, Khanutsanan Woranam, Jarckrit Jeeunngoi, Gulsiri Senawong
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Publicado: Nature Portfolio 2021
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spelling oai:doaj.org-article:47e493fcec8d4d03b5ed88541c970e422021-12-02T17:55:13ZAnticancer effects of the combined Thai noni juice ethanolic extracts and 5-fluorouracil against cholangiocarcinoma cells in vitro and in vivo10.1038/s41598-021-94049-z2045-2322https://doaj.org/article/47e493fcec8d4d03b5ed88541c970e422021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94049-zhttps://doaj.org/toc/2045-2322Abstract Application of 5-fluorouracil (5-FU) in cholangiocarcinoma (CCA) is limited by adverse side effects and chemoresistance. Therefore, the combination therapy of 5-FU with other substances, especially natural products may provide a new strategy for CCA treatment. The aim of this study was to evaluate the combination effects of 5-FU and two ethanolic extracts of Thai noni juice (TNJ) products on CCA cell lines and nude mice xenografts. The results of antiproliferative assay showed the combination treatment of 5-FU and each TNJ ethanolic extract exerted more cytotoxicity on CCA cells than either single agent treatment. Synergistic effects of drug combinations can enable the dose reduction of 5-FU. The mechanism underlying a combination treatment was apoptosis induction through an activation of p53 and Bax proteins. In the nude mouse xenograft model, combination treatments of 5-FU with each TNJ ethanolic extract suppressed the growth of CCA cells implanted mice more than single agent treatments with no effects on mouse body weight, kidney, and spleen. Moreover, low doses of TNJ ethanolic extracts reduced the hepatotoxicity of 5-FU in nude mice. Taken together, these data suggested that the ethanolic extracts of TNJ products can enhance the anti-CCA effect and reduce toxicity of 5-FU.Jeerati PrompipakThanaset SenawongBanchob SripaAlbert J. KettermanSuppawit UtaiwatKhanutsanan WoranamJarckrit JeeunngoiGulsiri SenawongNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Jeerati Prompipak
Thanaset Senawong
Banchob Sripa
Albert J. Ketterman
Suppawit Utaiwat
Khanutsanan Woranam
Jarckrit Jeeunngoi
Gulsiri Senawong
Anticancer effects of the combined Thai noni juice ethanolic extracts and 5-fluorouracil against cholangiocarcinoma cells in vitro and in vivo
description Abstract Application of 5-fluorouracil (5-FU) in cholangiocarcinoma (CCA) is limited by adverse side effects and chemoresistance. Therefore, the combination therapy of 5-FU with other substances, especially natural products may provide a new strategy for CCA treatment. The aim of this study was to evaluate the combination effects of 5-FU and two ethanolic extracts of Thai noni juice (TNJ) products on CCA cell lines and nude mice xenografts. The results of antiproliferative assay showed the combination treatment of 5-FU and each TNJ ethanolic extract exerted more cytotoxicity on CCA cells than either single agent treatment. Synergistic effects of drug combinations can enable the dose reduction of 5-FU. The mechanism underlying a combination treatment was apoptosis induction through an activation of p53 and Bax proteins. In the nude mouse xenograft model, combination treatments of 5-FU with each TNJ ethanolic extract suppressed the growth of CCA cells implanted mice more than single agent treatments with no effects on mouse body weight, kidney, and spleen. Moreover, low doses of TNJ ethanolic extracts reduced the hepatotoxicity of 5-FU in nude mice. Taken together, these data suggested that the ethanolic extracts of TNJ products can enhance the anti-CCA effect and reduce toxicity of 5-FU.
format article
author Jeerati Prompipak
Thanaset Senawong
Banchob Sripa
Albert J. Ketterman
Suppawit Utaiwat
Khanutsanan Woranam
Jarckrit Jeeunngoi
Gulsiri Senawong
author_facet Jeerati Prompipak
Thanaset Senawong
Banchob Sripa
Albert J. Ketterman
Suppawit Utaiwat
Khanutsanan Woranam
Jarckrit Jeeunngoi
Gulsiri Senawong
author_sort Jeerati Prompipak
title Anticancer effects of the combined Thai noni juice ethanolic extracts and 5-fluorouracil against cholangiocarcinoma cells in vitro and in vivo
title_short Anticancer effects of the combined Thai noni juice ethanolic extracts and 5-fluorouracil against cholangiocarcinoma cells in vitro and in vivo
title_full Anticancer effects of the combined Thai noni juice ethanolic extracts and 5-fluorouracil against cholangiocarcinoma cells in vitro and in vivo
title_fullStr Anticancer effects of the combined Thai noni juice ethanolic extracts and 5-fluorouracil against cholangiocarcinoma cells in vitro and in vivo
title_full_unstemmed Anticancer effects of the combined Thai noni juice ethanolic extracts and 5-fluorouracil against cholangiocarcinoma cells in vitro and in vivo
title_sort anticancer effects of the combined thai noni juice ethanolic extracts and 5-fluorouracil against cholangiocarcinoma cells in vitro and in vivo
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/47e493fcec8d4d03b5ed88541c970e42
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