Nanopore long-read RNAseq reveals widespread transcriptional variation among the surface receptors of individual B cells
Short-read RNA-seq is limited in its ability to resolve complex transcript isoforms since it cannot sequence full-length cDNA. Here the authors use Oxford Nanopore MinION and their Mandalorion analysis pipeline to measure complex isoforms in B1a cells.
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Auteurs principaux: | , , , , , , , , , |
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Format: | article |
Langue: | EN |
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Nature Portfolio
2017
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Accès en ligne: | https://doaj.org/article/47e55c463ee54d15b838dd82d9d1d77f |
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Résumé: | Short-read RNA-seq is limited in its ability to resolve complex transcript isoforms since it cannot sequence full-length cDNA. Here the authors use Oxford Nanopore MinION and their Mandalorion analysis pipeline to measure complex isoforms in B1a cells. |
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