Nanopore long-read RNAseq reveals widespread transcriptional variation among the surface receptors of individual B cells

Short-read RNA-seq is limited in its ability to resolve complex transcript isoforms since it cannot sequence full-length cDNA. Here the authors use Oxford Nanopore MinION and their Mandalorion analysis pipeline to measure complex isoforms in B1a cells.

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Autores principales:	Ashley Byrne, Anna E. Beaudin, Hugh E. Olsen, Miten Jain, Charles Cole, Theron Palmer, Rebecca M. DuBois, E. Camilla Forsberg, Mark Akeson, Christopher Vollmers
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/47e55c463ee54d15b838dd82d9d1d77f
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spelling	oai:doaj.org-article:47e55c463ee54d15b838dd82d9d1d77f2021-12-02T15:38:53ZNanopore long-read RNAseq reveals widespread transcriptional variation among the surface receptors of individual B cells10.1038/ncomms160272041-1723https://doaj.org/article/47e55c463ee54d15b838dd82d9d1d77f2017-07-01T00:00:00Zhttps://doi.org/10.1038/ncomms16027https://doaj.org/toc/2041-1723Short-read RNA-seq is limited in its ability to resolve complex transcript isoforms since it cannot sequence full-length cDNA. Here the authors use Oxford Nanopore MinION and their Mandalorion analysis pipeline to measure complex isoforms in B1a cells.Ashley ByrneAnna E. BeaudinHugh E. OlsenMiten JainCharles ColeTheron PalmerRebecca M. DuBoisE. Camilla ForsbergMark AkesonChristopher VollmersNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Ashley Byrne Anna E. Beaudin Hugh E. Olsen Miten Jain Charles Cole Theron Palmer Rebecca M. DuBois E. Camilla Forsberg Mark Akeson Christopher Vollmers Nanopore long-read RNAseq reveals widespread transcriptional variation among the surface receptors of individual B cells
description	Short-read RNA-seq is limited in its ability to resolve complex transcript isoforms since it cannot sequence full-length cDNA. Here the authors use Oxford Nanopore MinION and their Mandalorion analysis pipeline to measure complex isoforms in B1a cells.
format	article
author	Ashley Byrne Anna E. Beaudin Hugh E. Olsen Miten Jain Charles Cole Theron Palmer Rebecca M. DuBois E. Camilla Forsberg Mark Akeson Christopher Vollmers
author_facet	Ashley Byrne Anna E. Beaudin Hugh E. Olsen Miten Jain Charles Cole Theron Palmer Rebecca M. DuBois E. Camilla Forsberg Mark Akeson Christopher Vollmers
author_sort	Ashley Byrne
title	Nanopore long-read RNAseq reveals widespread transcriptional variation among the surface receptors of individual B cells
title_short	Nanopore long-read RNAseq reveals widespread transcriptional variation among the surface receptors of individual B cells
title_full	Nanopore long-read RNAseq reveals widespread transcriptional variation among the surface receptors of individual B cells
title_fullStr	Nanopore long-read RNAseq reveals widespread transcriptional variation among the surface receptors of individual B cells
title_full_unstemmed	Nanopore long-read RNAseq reveals widespread transcriptional variation among the surface receptors of individual B cells
title_sort	nanopore long-read rnaseq reveals widespread transcriptional variation among the surface receptors of individual b cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/47e55c463ee54d15b838dd82d9d1d77f
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Nanopore long-read RNAseq reveals widespread transcriptional variation among the surface receptors of individual B cells

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