Nanopore long-read RNAseq reveals widespread transcriptional variation among the surface receptors of individual B cells
Short-read RNA-seq is limited in its ability to resolve complex transcript isoforms since it cannot sequence full-length cDNA. Here the authors use Oxford Nanopore MinION and their Mandalorion analysis pipeline to measure complex isoforms in B1a cells.
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Main Authors: | Ashley Byrne, Anna E. Beaudin, Hugh E. Olsen, Miten Jain, Charles Cole, Theron Palmer, Rebecca M. DuBois, E. Camilla Forsberg, Mark Akeson, Christopher Vollmers |
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Format: | article |
Language: | EN |
Published: |
Nature Portfolio
2017
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Online Access: | https://doaj.org/article/47e55c463ee54d15b838dd82d9d1d77f |
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