Proteins Potentially Involved in Immune Evasion Strategies in <italic toggle="yes">Sporothrix brasiliensis</italic> Elucidated by Ultra-High-Resolution Mass Spectrometry

ABSTRACT Sporothrix brasiliensis is the prevalent agent of a large zoonotic outbreak in Brazil. With the involvement of several thousands of cases, this is the largest cohort of human and animal sporotrichosis on record in the world. Infections are characterized by local cutaneous dissemination in h...

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Autores principales:	Luana Rossato, Leandro Ferreira Moreno, Azadeh Jamalian, Benjamin Stielow, Sandro Rogério de Almeida, Sybren de Hoog, Joanna Freeke
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	Sporothrix brasiliensis Sporothrix schenckii genomics immune evasion proteomics virulence factors Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/47f61ac55cbf40629be621a67fa2c48f
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spelling	oai:doaj.org-article:47f61ac55cbf40629be621a67fa2c48f2021-11-15T15:24:22ZProteins Potentially Involved in Immune Evasion Strategies in <italic toggle="yes">Sporothrix brasiliensis</italic> Elucidated by Ultra-High-Resolution Mass Spectrometry10.1128/mSphere.00514-172379-5042https://doaj.org/article/47f61ac55cbf40629be621a67fa2c48f2018-06-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00514-17https://doaj.org/toc/2379-5042ABSTRACT Sporothrix brasiliensis is the prevalent agent of a large zoonotic outbreak in Brazil. With the involvement of several thousands of cases, this is the largest cohort of human and animal sporotrichosis on record in the world. Infections are characterized by local cutaneous dissemination in humans without underlying disease. S. brasiliensis has shown a high degree of virulence in a mouse model compared to the remaining Sporothrix species, including the ancestral species, Sporothrix schenckii. The present paper investigates a genomic and expressed-proteome comparison of S. brasiliensis to S. schenckii. Using bottom-up proteomics, we found 60 proteins exclusively expressed in S. brasiliensis. No significant genomic differences were found among the genes coding for this protein set. A comparison with literature data identified nine proteins that are known to be involved in virulence and immune evasion in other species, several of which had not yet been reported for the Sporothrix species analyzed. IMPORTANCE Sporotrichosis is an important disease in Brazil that is caused by fungi of the genus Sporothrix and affects cats and humans. Our work investigated the proteins differentially expressed by S. brasiliensis in order to find out why this species is more virulent and pathogenic than S. schenckii. We verified a set of proteins that may be related to immune escape and that can explain the high virulence.Luana RossatoLeandro Ferreira MorenoAzadeh JamalianBenjamin StielowSandro Rogério de AlmeidaSybren de HoogJoanna FreekeAmerican Society for MicrobiologyarticleSporothrix brasiliensisSporothrix schenckiigenomicsimmune evasionproteomicsvirulence factorsMicrobiologyQR1-502ENmSphere, Vol 3, Iss 3 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Sporothrix brasiliensis Sporothrix schenckii genomics immune evasion proteomics virulence factors Microbiology QR1-502
spellingShingle	Sporothrix brasiliensis Sporothrix schenckii genomics immune evasion proteomics virulence factors Microbiology QR1-502 Luana Rossato Leandro Ferreira Moreno Azadeh Jamalian Benjamin Stielow Sandro Rogério de Almeida Sybren de Hoog Joanna Freeke Proteins Potentially Involved in Immune Evasion Strategies in <italic toggle="yes">Sporothrix brasiliensis</italic> Elucidated by Ultra-High-Resolution Mass Spectrometry
description	ABSTRACT Sporothrix brasiliensis is the prevalent agent of a large zoonotic outbreak in Brazil. With the involvement of several thousands of cases, this is the largest cohort of human and animal sporotrichosis on record in the world. Infections are characterized by local cutaneous dissemination in humans without underlying disease. S. brasiliensis has shown a high degree of virulence in a mouse model compared to the remaining Sporothrix species, including the ancestral species, Sporothrix schenckii. The present paper investigates a genomic and expressed-proteome comparison of S. brasiliensis to S. schenckii. Using bottom-up proteomics, we found 60 proteins exclusively expressed in S. brasiliensis. No significant genomic differences were found among the genes coding for this protein set. A comparison with literature data identified nine proteins that are known to be involved in virulence and immune evasion in other species, several of which had not yet been reported for the Sporothrix species analyzed. IMPORTANCE Sporotrichosis is an important disease in Brazil that is caused by fungi of the genus Sporothrix and affects cats and humans. Our work investigated the proteins differentially expressed by S. brasiliensis in order to find out why this species is more virulent and pathogenic than S. schenckii. We verified a set of proteins that may be related to immune escape and that can explain the high virulence.
format	article
author	Luana Rossato Leandro Ferreira Moreno Azadeh Jamalian Benjamin Stielow Sandro Rogério de Almeida Sybren de Hoog Joanna Freeke
author_facet	Luana Rossato Leandro Ferreira Moreno Azadeh Jamalian Benjamin Stielow Sandro Rogério de Almeida Sybren de Hoog Joanna Freeke
author_sort	Luana Rossato
title	Proteins Potentially Involved in Immune Evasion Strategies in <italic toggle="yes">Sporothrix brasiliensis</italic> Elucidated by Ultra-High-Resolution Mass Spectrometry
title_short	Proteins Potentially Involved in Immune Evasion Strategies in <italic toggle="yes">Sporothrix brasiliensis</italic> Elucidated by Ultra-High-Resolution Mass Spectrometry
title_full	Proteins Potentially Involved in Immune Evasion Strategies in <italic toggle="yes">Sporothrix brasiliensis</italic> Elucidated by Ultra-High-Resolution Mass Spectrometry
title_fullStr	Proteins Potentially Involved in Immune Evasion Strategies in <italic toggle="yes">Sporothrix brasiliensis</italic> Elucidated by Ultra-High-Resolution Mass Spectrometry
title_full_unstemmed	Proteins Potentially Involved in Immune Evasion Strategies in <italic toggle="yes">Sporothrix brasiliensis</italic> Elucidated by Ultra-High-Resolution Mass Spectrometry
title_sort	proteins potentially involved in immune evasion strategies in <italic toggle="yes">sporothrix brasiliensis</italic> elucidated by ultra-high-resolution mass spectrometry
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/47f61ac55cbf40629be621a67fa2c48f
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Proteins Potentially Involved in Immune Evasion Strategies in <italic toggle="yes">Sporothrix brasiliensis</italic> Elucidated by Ultra-High-Resolution Mass Spectrometry

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