Fast and multiplexed superresolution imaging with DNA-PAINT-ERS

DNA-PAINT is a powerful super-resolution imaging method but is limited in speed due to slow exchange kinetics of the imaging strand. Here the authors present a method involving the addition of ethylene carbonate to the imaging buffer and modifications to the docking strand to improve the quality and...

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Autores principales:	Fehmi Civitci, Julia Shangguan, Ting Zheng, Kai Tao, Matthew Rames, John Kenison, Ying Zhang, Lei Wu, Carey Phelps, Sadik Esener, Xiaolin Nan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/47f84aeffbbf4a77995a5d88c6531e37
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spelling	oai:doaj.org-article:47f84aeffbbf4a77995a5d88c6531e372021-12-02T18:53:11ZFast and multiplexed superresolution imaging with DNA-PAINT-ERS10.1038/s41467-020-18181-62041-1723https://doaj.org/article/47f84aeffbbf4a77995a5d88c6531e372020-08-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-18181-6https://doaj.org/toc/2041-1723DNA-PAINT is a powerful super-resolution imaging method but is limited in speed due to slow exchange kinetics of the imaging strand. Here the authors present a method involving the addition of ethylene carbonate to the imaging buffer and modifications to the docking strand to improve the quality and speed of DNA-PAINT.Fehmi CivitciJulia ShangguanTing ZhengKai TaoMatthew RamesJohn KenisonYing ZhangLei WuCarey PhelpsSadik EsenerXiaolin NanNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-8 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Fehmi Civitci Julia Shangguan Ting Zheng Kai Tao Matthew Rames John Kenison Ying Zhang Lei Wu Carey Phelps Sadik Esener Xiaolin Nan Fast and multiplexed superresolution imaging with DNA-PAINT-ERS
description	DNA-PAINT is a powerful super-resolution imaging method but is limited in speed due to slow exchange kinetics of the imaging strand. Here the authors present a method involving the addition of ethylene carbonate to the imaging buffer and modifications to the docking strand to improve the quality and speed of DNA-PAINT.
format	article
author	Fehmi Civitci Julia Shangguan Ting Zheng Kai Tao Matthew Rames John Kenison Ying Zhang Lei Wu Carey Phelps Sadik Esener Xiaolin Nan
author_facet	Fehmi Civitci Julia Shangguan Ting Zheng Kai Tao Matthew Rames John Kenison Ying Zhang Lei Wu Carey Phelps Sadik Esener Xiaolin Nan
author_sort	Fehmi Civitci
title	Fast and multiplexed superresolution imaging with DNA-PAINT-ERS
title_short	Fast and multiplexed superresolution imaging with DNA-PAINT-ERS
title_full	Fast and multiplexed superresolution imaging with DNA-PAINT-ERS
title_fullStr	Fast and multiplexed superresolution imaging with DNA-PAINT-ERS
title_full_unstemmed	Fast and multiplexed superresolution imaging with DNA-PAINT-ERS
title_sort	fast and multiplexed superresolution imaging with dna-paint-ers
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/47f84aeffbbf4a77995a5d88c6531e37
work_keys_str_mv	AT fehmicivitci fastandmultiplexedsuperresolutionimagingwithdnapainters AT juliashangguan fastandmultiplexedsuperresolutionimagingwithdnapainters AT tingzheng fastandmultiplexedsuperresolutionimagingwithdnapainters AT kaitao fastandmultiplexedsuperresolutionimagingwithdnapainters AT matthewrames fastandmultiplexedsuperresolutionimagingwithdnapainters AT johnkenison fastandmultiplexedsuperresolutionimagingwithdnapainters AT yingzhang fastandmultiplexedsuperresolutionimagingwithdnapainters AT leiwu fastandmultiplexedsuperresolutionimagingwithdnapainters AT careyphelps fastandmultiplexedsuperresolutionimagingwithdnapainters AT sadikesener fastandmultiplexedsuperresolutionimagingwithdnapainters AT xiaolinnan fastandmultiplexedsuperresolutionimagingwithdnapainters
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Fast and multiplexed superresolution imaging with DNA-PAINT-ERS

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