Escherichia coli outer membrane vesicles can contribute to sepsis induced cardiac dysfunction

Abstract Sepsis induced cardiac dysfunction (SIC) is a severe complication to sepsis which significantly worsens patient outcomes. It is known that bacteria have the capacity to release outer membrane vesicles (OMVs), which are nano-sized bilayered vesicles composed of lipids and proteins, that can...

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Autores principales:	Kristina Svennerholm, Kyong-Su Park, Johannes Wikström, Cecilia Lässer, Rossella Crescitelli, Ganesh V. Shelke, Su Chul Jang, Shintaro Suzuki, Elga Bandeira, Charlotta S. Olofsson, Jan Lötvall
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/47f9c26689ee4c4f95a93df9f73256b7
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spelling	oai:doaj.org-article:47f9c26689ee4c4f95a93df9f73256b72021-12-02T15:05:28ZEscherichia coli outer membrane vesicles can contribute to sepsis induced cardiac dysfunction10.1038/s41598-017-16363-92045-2322https://doaj.org/article/47f9c26689ee4c4f95a93df9f73256b72017-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-16363-9https://doaj.org/toc/2045-2322Abstract Sepsis induced cardiac dysfunction (SIC) is a severe complication to sepsis which significantly worsens patient outcomes. It is known that bacteria have the capacity to release outer membrane vesicles (OMVs), which are nano-sized bilayered vesicles composed of lipids and proteins, that can induce a fatal inflammatory response. The aim of this study was to determine whether OMVs from a uropathogenic Escherichia coli strain can induce cardiac dysfunction, and to elucidate any mechanisms involved. OMVs induced irregular Ca2+ oscillations with a decreased frequency in cardiomyocytes through recordings of intracellular Ca2+ dynamics. Mice were intraperitoneally injected with bacteria-free OMVs, which resulted in increased concentration of pro-inflammatory cytokine levels in blood. Cytokines were increased in heart lysates, and OMVs could be detected in the heart after OMVs injection. Troponin T was significantly increased in blood, and echocardiography showed increased heart wall thickness as well as increased heart rate. This study shows that E. coli OMVs induce cardiac injury in vitro and in vivo, in the absence of bacteria, and may be a causative microbial signal in SIC. The role of OMVs in clinical disease warrant further studies, as bacterial OMVs in addition to live bacteria may be good therapeutic targets to control sepsis.Kristina SvennerholmKyong-Su ParkJohannes WikströmCecilia LässerRossella CrescitelliGanesh V. ShelkeSu Chul JangShintaro SuzukiElga BandeiraCharlotta S. OlofssonJan LötvallNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kristina Svennerholm Kyong-Su Park Johannes Wikström Cecilia Lässer Rossella Crescitelli Ganesh V. Shelke Su Chul Jang Shintaro Suzuki Elga Bandeira Charlotta S. Olofsson Jan Lötvall Escherichia coli outer membrane vesicles can contribute to sepsis induced cardiac dysfunction
description	Abstract Sepsis induced cardiac dysfunction (SIC) is a severe complication to sepsis which significantly worsens patient outcomes. It is known that bacteria have the capacity to release outer membrane vesicles (OMVs), which are nano-sized bilayered vesicles composed of lipids and proteins, that can induce a fatal inflammatory response. The aim of this study was to determine whether OMVs from a uropathogenic Escherichia coli strain can induce cardiac dysfunction, and to elucidate any mechanisms involved. OMVs induced irregular Ca2+ oscillations with a decreased frequency in cardiomyocytes through recordings of intracellular Ca2+ dynamics. Mice were intraperitoneally injected with bacteria-free OMVs, which resulted in increased concentration of pro-inflammatory cytokine levels in blood. Cytokines were increased in heart lysates, and OMVs could be detected in the heart after OMVs injection. Troponin T was significantly increased in blood, and echocardiography showed increased heart wall thickness as well as increased heart rate. This study shows that E. coli OMVs induce cardiac injury in vitro and in vivo, in the absence of bacteria, and may be a causative microbial signal in SIC. The role of OMVs in clinical disease warrant further studies, as bacterial OMVs in addition to live bacteria may be good therapeutic targets to control sepsis.
format	article
author	Kristina Svennerholm Kyong-Su Park Johannes Wikström Cecilia Lässer Rossella Crescitelli Ganesh V. Shelke Su Chul Jang Shintaro Suzuki Elga Bandeira Charlotta S. Olofsson Jan Lötvall
author_facet	Kristina Svennerholm Kyong-Su Park Johannes Wikström Cecilia Lässer Rossella Crescitelli Ganesh V. Shelke Su Chul Jang Shintaro Suzuki Elga Bandeira Charlotta S. Olofsson Jan Lötvall
author_sort	Kristina Svennerholm
title	Escherichia coli outer membrane vesicles can contribute to sepsis induced cardiac dysfunction
title_short	Escherichia coli outer membrane vesicles can contribute to sepsis induced cardiac dysfunction
title_full	Escherichia coli outer membrane vesicles can contribute to sepsis induced cardiac dysfunction
title_fullStr	Escherichia coli outer membrane vesicles can contribute to sepsis induced cardiac dysfunction
title_full_unstemmed	Escherichia coli outer membrane vesicles can contribute to sepsis induced cardiac dysfunction
title_sort	escherichia coli outer membrane vesicles can contribute to sepsis induced cardiac dysfunction
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/47f9c26689ee4c4f95a93df9f73256b7
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Escherichia coli outer membrane vesicles can contribute to sepsis induced cardiac dysfunction

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