Associations of Polymorphisms Localized in the 3′UTR Regions of the KRAS, NRAS, MAPK1 Genes with Laryngeal Squamous Cell Carcinoma

Background: Genetic variations, localized in the 3′ untranslated region (UTR) in mitogen-activated protein kinase (MAPK) pathway-related genes, may alter the transcription and impact the pathogenesis of laryngeal squamous cell carcinoma (LSCC). The present study investigated the associations of sing...

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Autores principales:	Ruta Insodaite, Alina Smalinskiene, Vykintas Liutkevicius, Virgilijus Ulozas, Roberta Poceviciute, Arunas Bielevicius, Laimutis Kucinskas
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	laryngeal squamous cell carcinoma single-nucleotide polymorphisms mitogen-activated protein kinases <i>KRAS</i> <i>NRAS</i> <i>MAPK1</i> Genetics QH426-470
Acceso en línea:	https://doaj.org/article/480076e31f594edbb19f08fad2adf232
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spelling	oai:doaj.org-article:480076e31f594edbb19f08fad2adf2322021-11-25T17:40:41ZAssociations of Polymorphisms Localized in the 3′UTR Regions of the <i>KRAS</i>, <i>NRAS</i>, <i>MAPK1</i> Genes with Laryngeal Squamous Cell Carcinoma10.3390/genes121116792073-4425https://doaj.org/article/480076e31f594edbb19f08fad2adf2322021-10-01T00:00:00Zhttps://www.mdpi.com/2073-4425/12/11/1679https://doaj.org/toc/2073-4425Background: Genetic variations, localized in the 3′ untranslated region (UTR) in mitogen-activated protein kinase (MAPK) pathway-related genes, may alter the transcription and impact the pathogenesis of laryngeal squamous cell carcinoma (LSCC). The present study investigated the associations of single-nucleotide polymorphisms (SNP), localized in the 3′UTR) of the <i>KRAS</i>, <i>NRAS,</i> and <i>MAPK1</i> genes with LSCC risk and clinicopathological features. Methods: Genomic DNA and clinical data were collected from 327 adult men with LSCC. The control group was formed from 333 healthy men. Genotyping of the SNPs was performed using TaqMan SNP genotyping assays. Five <i>KRAS</i>, <i>NRAS,</i> and <i>MAPK1</i> polymorphisms were analyzed. All studied genotypes were in Hardy–Weinberg equilibrium and had the same allele distribution as the 1000 Genomes project Phase 3 dataset for the European population. Results: Significant associations of the studied SNPs with reduced LSCC risk were observed between <i>NRAS</i> rs14804 major genotype CC. Significant associations of the studied SNPs with clinicopathologic variables were also observed between <i>NRAS</i> rs14804 minor T allele and advanced tumor stage and positive lymph node status. SNP of <i>MAPK1</i> rs9340 was associated with distant metastasis. Moreover, haplotype analysis of two <i>KRAS</i> SNPs rs712 and rs7973450 revealed that TG haplotype was associated with positive lymph node status in LSCC patients. Conclusions: According to the present study, 3′UTR SNP in the <i>NRAS</i> and <i>MAPK1</i> genes may contribute to the identifications of patients at higher risk of LSCC lymph node and distant metastasis development.Ruta InsodaiteAlina SmalinskieneVykintas LiutkeviciusVirgilijus UlozasRoberta PoceviciuteArunas BieleviciusLaimutis KucinskasMDPI AGarticlelaryngeal squamous cell carcinomasingle-nucleotide polymorphismsmitogen-activated protein kinases<i>KRAS</i><i>NRAS</i><i>MAPK1</i>GeneticsQH426-470ENGenes, Vol 12, Iss 1679, p 1679 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	laryngeal squamous cell carcinoma single-nucleotide polymorphisms mitogen-activated protein kinases <i>KRAS</i> <i>NRAS</i> <i>MAPK1</i> Genetics QH426-470
spellingShingle	laryngeal squamous cell carcinoma single-nucleotide polymorphisms mitogen-activated protein kinases <i>KRAS</i> <i>NRAS</i> <i>MAPK1</i> Genetics QH426-470 Ruta Insodaite Alina Smalinskiene Vykintas Liutkevicius Virgilijus Ulozas Roberta Poceviciute Arunas Bielevicius Laimutis Kucinskas Associations of Polymorphisms Localized in the 3′UTR Regions of the <i>KRAS</i>, <i>NRAS</i>, <i>MAPK1</i> Genes with Laryngeal Squamous Cell Carcinoma
description	Background: Genetic variations, localized in the 3′ untranslated region (UTR) in mitogen-activated protein kinase (MAPK) pathway-related genes, may alter the transcription and impact the pathogenesis of laryngeal squamous cell carcinoma (LSCC). The present study investigated the associations of single-nucleotide polymorphisms (SNP), localized in the 3′UTR) of the <i>KRAS</i>, <i>NRAS,</i> and <i>MAPK1</i> genes with LSCC risk and clinicopathological features. Methods: Genomic DNA and clinical data were collected from 327 adult men with LSCC. The control group was formed from 333 healthy men. Genotyping of the SNPs was performed using TaqMan SNP genotyping assays. Five <i>KRAS</i>, <i>NRAS,</i> and <i>MAPK1</i> polymorphisms were analyzed. All studied genotypes were in Hardy–Weinberg equilibrium and had the same allele distribution as the 1000 Genomes project Phase 3 dataset for the European population. Results: Significant associations of the studied SNPs with reduced LSCC risk were observed between <i>NRAS</i> rs14804 major genotype CC. Significant associations of the studied SNPs with clinicopathologic variables were also observed between <i>NRAS</i> rs14804 minor T allele and advanced tumor stage and positive lymph node status. SNP of <i>MAPK1</i> rs9340 was associated with distant metastasis. Moreover, haplotype analysis of two <i>KRAS</i> SNPs rs712 and rs7973450 revealed that TG haplotype was associated with positive lymph node status in LSCC patients. Conclusions: According to the present study, 3′UTR SNP in the <i>NRAS</i> and <i>MAPK1</i> genes may contribute to the identifications of patients at higher risk of LSCC lymph node and distant metastasis development.
format	article
author	Ruta Insodaite Alina Smalinskiene Vykintas Liutkevicius Virgilijus Ulozas Roberta Poceviciute Arunas Bielevicius Laimutis Kucinskas
author_facet	Ruta Insodaite Alina Smalinskiene Vykintas Liutkevicius Virgilijus Ulozas Roberta Poceviciute Arunas Bielevicius Laimutis Kucinskas
author_sort	Ruta Insodaite
title	Associations of Polymorphisms Localized in the 3′UTR Regions of the <i>KRAS</i>, <i>NRAS</i>, <i>MAPK1</i> Genes with Laryngeal Squamous Cell Carcinoma
title_short	Associations of Polymorphisms Localized in the 3′UTR Regions of the <i>KRAS</i>, <i>NRAS</i>, <i>MAPK1</i> Genes with Laryngeal Squamous Cell Carcinoma
title_full	Associations of Polymorphisms Localized in the 3′UTR Regions of the <i>KRAS</i>, <i>NRAS</i>, <i>MAPK1</i> Genes with Laryngeal Squamous Cell Carcinoma
title_fullStr	Associations of Polymorphisms Localized in the 3′UTR Regions of the <i>KRAS</i>, <i>NRAS</i>, <i>MAPK1</i> Genes with Laryngeal Squamous Cell Carcinoma
title_full_unstemmed	Associations of Polymorphisms Localized in the 3′UTR Regions of the <i>KRAS</i>, <i>NRAS</i>, <i>MAPK1</i> Genes with Laryngeal Squamous Cell Carcinoma
title_sort	associations of polymorphisms localized in the 3′utr regions of the <i>kras</i>, <i>nras</i>, <i>mapk1</i> genes with laryngeal squamous cell carcinoma
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/480076e31f594edbb19f08fad2adf232
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Associations of Polymorphisms Localized in the 3′UTR Regions of the <i>KRAS</i>, <i>NRAS</i>, <i>MAPK1</i> Genes with Laryngeal Squamous Cell Carcinoma

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