Chronic Low-Dose Alcohol Consumption Promotes Cerebral Angiogenesis in Mice

Chronic alcohol consumption dose-dependently affects the incidence and prognosis of ischemic stroke. We determined the influence of chronic alcohol consumption on cerebral angiogenesis under physiological conditions and following ischemic stroke. In in vitro studies, acute exposure to low-concentrat...

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Autores principales:	Jiyu Li, Chun Li, Ethyn G. Loreno, Sumitra Miriyala, Manikandan Panchatcharam, Xiaohong Lu, Hong Sun
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	alcohol angiogenesis ischemic stroke VEGF-A VEGFR2 brain Diseases of the circulatory (Cardiovascular) system RC666-701
Acceso en línea:	https://doaj.org/article/4803d1a38d35445cbe4207d8eaccc77e
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spelling	oai:doaj.org-article:4803d1a38d35445cbe4207d8eaccc77e2021-11-17T05:03:54ZChronic Low-Dose Alcohol Consumption Promotes Cerebral Angiogenesis in Mice2297-055X10.3389/fcvm.2021.681627https://doaj.org/article/4803d1a38d35445cbe4207d8eaccc77e2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcvm.2021.681627/fullhttps://doaj.org/toc/2297-055XChronic alcohol consumption dose-dependently affects the incidence and prognosis of ischemic stroke. We determined the influence of chronic alcohol consumption on cerebral angiogenesis under physiological conditions and following ischemic stroke. In in vitro studies, acute exposure to low-concentration ethanol significantly increased angiogenic capability and upregulated vascular endothelial growth factor A (VEGF-A) and vascular endothelial growth factor receptor 2 (VEGFR2) in C57BL/6J mouse brain microvascular endothelial cells (MBMVECs). The increased angiogenic capability was abolished in the presence of a VEGFR2 inhibitor. In addition, the increased angiogenic capability and upregulated VEGF-A and VEGFR2 remained in chronically low-concentration ethanol-exposed MBMVECs. In in vivo studies, 8-week gavage feeding with low-dose ethanol significantly increased vessel density and vessel branches and upregulated VEGF-A and VEGFR2 in the cerebral cortex under physiological conditions. Furthermore, vessel density, vessel branches, and expression of VEGF-A and VEGFR2 in the peri-infarct cortex were significantly greater in low-dose ethanol-fed mice at 72 h of reperfusion. Although low-dose ethanol did not alter cerebral vasoreactivity and regional cerebral blood flow (rCBF) either before or during ischemia, it significantly augmented post-ischemic hyperemia during reperfusion. In contrast, exposure to high-concentration ethanol and 8-week gavage feeding with high-dose ethanol only had a mild inhibitory effect on angiogenic capability and cerebral angiogenesis, respectively. We conclude that heavy alcohol consumption may not dramatically alter cerebral angiogenesis, whereas light alcohol consumption significantly promotes cerebral angiogenesis.Jiyu LiChun LiEthyn G. LorenoSumitra MiriyalaManikandan PanchatcharamXiaohong LuHong SunFrontiers Media S.A.articlealcoholangiogenesisischemic strokeVEGF-AVEGFR2brainDiseases of the circulatory (Cardiovascular) systemRC666-701ENFrontiers in Cardiovascular Medicine, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	alcohol angiogenesis ischemic stroke VEGF-A VEGFR2 brain Diseases of the circulatory (Cardiovascular) system RC666-701
spellingShingle	alcohol angiogenesis ischemic stroke VEGF-A VEGFR2 brain Diseases of the circulatory (Cardiovascular) system RC666-701 Jiyu Li Chun Li Ethyn G. Loreno Sumitra Miriyala Manikandan Panchatcharam Xiaohong Lu Hong Sun Chronic Low-Dose Alcohol Consumption Promotes Cerebral Angiogenesis in Mice
description	Chronic alcohol consumption dose-dependently affects the incidence and prognosis of ischemic stroke. We determined the influence of chronic alcohol consumption on cerebral angiogenesis under physiological conditions and following ischemic stroke. In in vitro studies, acute exposure to low-concentration ethanol significantly increased angiogenic capability and upregulated vascular endothelial growth factor A (VEGF-A) and vascular endothelial growth factor receptor 2 (VEGFR2) in C57BL/6J mouse brain microvascular endothelial cells (MBMVECs). The increased angiogenic capability was abolished in the presence of a VEGFR2 inhibitor. In addition, the increased angiogenic capability and upregulated VEGF-A and VEGFR2 remained in chronically low-concentration ethanol-exposed MBMVECs. In in vivo studies, 8-week gavage feeding with low-dose ethanol significantly increased vessel density and vessel branches and upregulated VEGF-A and VEGFR2 in the cerebral cortex under physiological conditions. Furthermore, vessel density, vessel branches, and expression of VEGF-A and VEGFR2 in the peri-infarct cortex were significantly greater in low-dose ethanol-fed mice at 72 h of reperfusion. Although low-dose ethanol did not alter cerebral vasoreactivity and regional cerebral blood flow (rCBF) either before or during ischemia, it significantly augmented post-ischemic hyperemia during reperfusion. In contrast, exposure to high-concentration ethanol and 8-week gavage feeding with high-dose ethanol only had a mild inhibitory effect on angiogenic capability and cerebral angiogenesis, respectively. We conclude that heavy alcohol consumption may not dramatically alter cerebral angiogenesis, whereas light alcohol consumption significantly promotes cerebral angiogenesis.
format	article
author	Jiyu Li Chun Li Ethyn G. Loreno Sumitra Miriyala Manikandan Panchatcharam Xiaohong Lu Hong Sun
author_facet	Jiyu Li Chun Li Ethyn G. Loreno Sumitra Miriyala Manikandan Panchatcharam Xiaohong Lu Hong Sun
author_sort	Jiyu Li
title	Chronic Low-Dose Alcohol Consumption Promotes Cerebral Angiogenesis in Mice
title_short	Chronic Low-Dose Alcohol Consumption Promotes Cerebral Angiogenesis in Mice
title_full	Chronic Low-Dose Alcohol Consumption Promotes Cerebral Angiogenesis in Mice
title_fullStr	Chronic Low-Dose Alcohol Consumption Promotes Cerebral Angiogenesis in Mice
title_full_unstemmed	Chronic Low-Dose Alcohol Consumption Promotes Cerebral Angiogenesis in Mice
title_sort	chronic low-dose alcohol consumption promotes cerebral angiogenesis in mice
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/4803d1a38d35445cbe4207d8eaccc77e
work_keys_str_mv	AT jiyuli chroniclowdosealcoholconsumptionpromotescerebralangiogenesisinmice AT chunli chroniclowdosealcoholconsumptionpromotescerebralangiogenesisinmice AT ethyngloreno chroniclowdosealcoholconsumptionpromotescerebralangiogenesisinmice AT sumitramiriyala chroniclowdosealcoholconsumptionpromotescerebralangiogenesisinmice AT manikandanpanchatcharam chroniclowdosealcoholconsumptionpromotescerebralangiogenesisinmice AT xiaohonglu chroniclowdosealcoholconsumptionpromotescerebralangiogenesisinmice AT hongsun chroniclowdosealcoholconsumptionpromotescerebralangiogenesisinmice
_version_	1718425921341358080

Chronic Low-Dose Alcohol Consumption Promotes Cerebral Angiogenesis in Mice

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