Simulation of hypoxia of myocardial cells in microfluidic systems

Abstract The paper presents a newly designed microfluidic system that allows simulation of myocardial hypoxia by biochemical method. The geometry of the microsystem was designed in such a way, that quantitative fluorescent measurements using a spectrofluorometric plate reader was possible. Biochemic...

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Autores principales:	Anna Kobuszewska, Elżbieta Jastrzębska, Kamil Żukowski, Zbigniew Brzózka
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4803df6aa5414c97b3eb9a11305ec2ba
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spelling	oai:doaj.org-article:4803df6aa5414c97b3eb9a11305ec2ba2021-12-02T18:48:08ZSimulation of hypoxia of myocardial cells in microfluidic systems10.1038/s41598-020-72660-w2045-2322https://doaj.org/article/4803df6aa5414c97b3eb9a11305ec2ba2020-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-72660-whttps://doaj.org/toc/2045-2322Abstract The paper presents a newly designed microfluidic system that allows simulation of myocardial hypoxia by biochemical method. The geometry of the microsystem was designed in such a way, that quantitative fluorescent measurements using a spectrofluorometric plate reader was possible. Biochemical simulation of hypoxia was carried out using potent mitochondrial oxidative phosphorylation uncoupler—Carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone (FCCP). Two cardiac cell lines were used in the study—rat cardiomyoblasts (H9C2) and human cardiomyocytes. The effectiveness of biochemical simulation of hypoxia was studied using two fluorescent dyes: carbocyanine iodide (JC-1) and Fluo-4. Changes in the mitochondrial membrane potential and concentration of intracellular calcium ions were tested. The major novelty of this research was the applying the microfluidic system to create hypoxia conditions for cardiac cells using the biochemical approach. In further studies, the presented hypoxia model could be used to develop new methods of treatment of ischemic heart disease for example in cell therapy based on stem cells.Anna KobuszewskaElżbieta JastrzębskaKamil ŻukowskiZbigniew BrzózkaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anna Kobuszewska Elżbieta Jastrzębska Kamil Żukowski Zbigniew Brzózka Simulation of hypoxia of myocardial cells in microfluidic systems
description	Abstract The paper presents a newly designed microfluidic system that allows simulation of myocardial hypoxia by biochemical method. The geometry of the microsystem was designed in such a way, that quantitative fluorescent measurements using a spectrofluorometric plate reader was possible. Biochemical simulation of hypoxia was carried out using potent mitochondrial oxidative phosphorylation uncoupler—Carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone (FCCP). Two cardiac cell lines were used in the study—rat cardiomyoblasts (H9C2) and human cardiomyocytes. The effectiveness of biochemical simulation of hypoxia was studied using two fluorescent dyes: carbocyanine iodide (JC-1) and Fluo-4. Changes in the mitochondrial membrane potential and concentration of intracellular calcium ions were tested. The major novelty of this research was the applying the microfluidic system to create hypoxia conditions for cardiac cells using the biochemical approach. In further studies, the presented hypoxia model could be used to develop new methods of treatment of ischemic heart disease for example in cell therapy based on stem cells.
format	article
author	Anna Kobuszewska Elżbieta Jastrzębska Kamil Żukowski Zbigniew Brzózka
author_facet	Anna Kobuszewska Elżbieta Jastrzębska Kamil Żukowski Zbigniew Brzózka
author_sort	Anna Kobuszewska
title	Simulation of hypoxia of myocardial cells in microfluidic systems
title_short	Simulation of hypoxia of myocardial cells in microfluidic systems
title_full	Simulation of hypoxia of myocardial cells in microfluidic systems
title_fullStr	Simulation of hypoxia of myocardial cells in microfluidic systems
title_full_unstemmed	Simulation of hypoxia of myocardial cells in microfluidic systems
title_sort	simulation of hypoxia of myocardial cells in microfluidic systems
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/4803df6aa5414c97b3eb9a11305ec2ba
work_keys_str_mv	AT annakobuszewska simulationofhypoxiaofmyocardialcellsinmicrofluidicsystems AT elzbietajastrzebska simulationofhypoxiaofmyocardialcellsinmicrofluidicsystems AT kamilzukowski simulationofhypoxiaofmyocardialcellsinmicrofluidicsystems AT zbigniewbrzozka simulationofhypoxiaofmyocardialcellsinmicrofluidicsystems
_version_	1718377619080085504

Simulation of hypoxia of myocardial cells in microfluidic systems

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