Human placental piwi-interacting RNA transcriptome is characterized by expression from the DLK1-DIO3 imprinted region

Abstract The placenta is vital to embryonic development and requires a finely-tuned pattern of gene expression, achieved in part by its unique epigenetic landscape. Piwi-interacting RNAs (piRNAs) are a class of small-non-coding RNA with established roles as epigenetic regulators of gene expression,...

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Autores principales:	Victor D. Martinez, Adam P. Sage, Brenda C. Minatel, Erin A. Marshall, E. Magda Price, Daiana D. Becker-Santos, Wendy P. Robinson, Wan L. Lam
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4806d1b9d0fd42b4979aeb71fa7166a4
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spelling	oai:doaj.org-article:4806d1b9d0fd42b4979aeb71fa7166a42021-12-02T16:17:34ZHuman placental piwi-interacting RNA transcriptome is characterized by expression from the DLK1-DIO3 imprinted region10.1038/s41598-021-93885-32045-2322https://doaj.org/article/4806d1b9d0fd42b4979aeb71fa7166a42021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93885-3https://doaj.org/toc/2045-2322Abstract The placenta is vital to embryonic development and requires a finely-tuned pattern of gene expression, achieved in part by its unique epigenetic landscape. Piwi-interacting RNAs (piRNAs) are a class of small-non-coding RNA with established roles as epigenetic regulators of gene expression, largely via methylation of targeted DNA sequences. The expression of piRNAs have mainly been described in germ cells, but a fraction have been shown to retain expression in adult somatic tissues. To aid in understanding the contribution of these regulators in the placenta, we provide the first description of the piRNA transcriptome in human placentas. We find 297 piRNAs to be preferentially expressed in the human placenta, a subset of which are expressed at higher levels relative to testes samples. We also observed a large proportion of placental piRNAs to be expressed from a single locus, as distinct from canonical cluster locations associated with transposable element silencing. Finally, we find that 15 of the highest-expressed placental piRNAs maps to the DLK1-DIO3 locus, suggesting a link to placental biology. Our findings suggest that piRNAs could contribute to the molecular networks defining placental function in humans, and a biological impact of piRNA expression beyond germ cells.Victor D. MartinezAdam P. SageBrenda C. MinatelErin A. MarshallE. Magda PriceDaiana D. Becker-SantosWendy P. RobinsonWan L. LamNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Victor D. Martinez Adam P. Sage Brenda C. Minatel Erin A. Marshall E. Magda Price Daiana D. Becker-Santos Wendy P. Robinson Wan L. Lam Human placental piwi-interacting RNA transcriptome is characterized by expression from the DLK1-DIO3 imprinted region
description	Abstract The placenta is vital to embryonic development and requires a finely-tuned pattern of gene expression, achieved in part by its unique epigenetic landscape. Piwi-interacting RNAs (piRNAs) are a class of small-non-coding RNA with established roles as epigenetic regulators of gene expression, largely via methylation of targeted DNA sequences. The expression of piRNAs have mainly been described in germ cells, but a fraction have been shown to retain expression in adult somatic tissues. To aid in understanding the contribution of these regulators in the placenta, we provide the first description of the piRNA transcriptome in human placentas. We find 297 piRNAs to be preferentially expressed in the human placenta, a subset of which are expressed at higher levels relative to testes samples. We also observed a large proportion of placental piRNAs to be expressed from a single locus, as distinct from canonical cluster locations associated with transposable element silencing. Finally, we find that 15 of the highest-expressed placental piRNAs maps to the DLK1-DIO3 locus, suggesting a link to placental biology. Our findings suggest that piRNAs could contribute to the molecular networks defining placental function in humans, and a biological impact of piRNA expression beyond germ cells.
format	article
author	Victor D. Martinez Adam P. Sage Brenda C. Minatel Erin A. Marshall E. Magda Price Daiana D. Becker-Santos Wendy P. Robinson Wan L. Lam
author_facet	Victor D. Martinez Adam P. Sage Brenda C. Minatel Erin A. Marshall E. Magda Price Daiana D. Becker-Santos Wendy P. Robinson Wan L. Lam
author_sort	Victor D. Martinez
title	Human placental piwi-interacting RNA transcriptome is characterized by expression from the DLK1-DIO3 imprinted region
title_short	Human placental piwi-interacting RNA transcriptome is characterized by expression from the DLK1-DIO3 imprinted region
title_full	Human placental piwi-interacting RNA transcriptome is characterized by expression from the DLK1-DIO3 imprinted region
title_fullStr	Human placental piwi-interacting RNA transcriptome is characterized by expression from the DLK1-DIO3 imprinted region
title_full_unstemmed	Human placental piwi-interacting RNA transcriptome is characterized by expression from the DLK1-DIO3 imprinted region
title_sort	human placental piwi-interacting rna transcriptome is characterized by expression from the dlk1-dio3 imprinted region
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4806d1b9d0fd42b4979aeb71fa7166a4
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Human placental piwi-interacting RNA transcriptome is characterized by expression from the DLK1-DIO3 imprinted region

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