HETEROSUBTYPIC IMMUNE RESPONSE AND CROSS-PROTECTION AGAINST A HIGHLY PATHOGENIC A (H5N1) INFLUENZA VIRUS IN MICE IMMUNIZED WITH COLD-ADAPTED A/LENINGRAD/134/17/57 (H2N2) INFLUENZA VIRUS

While investigating the efficacy of an H5N2 ca reassortant vaccine candidate in protecting against a lethal challenge with a highly pathogenic (HP) H5N1 virus in the mouse model, we observed a degree of cross-protection provided by the ca Len/17 (H2N2) virus itself. 80% of mice administered a high d...

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Autores principales:	A. R. Rekstin, J. A. Desheva, X. Lu, G. I. Alexandrova, A. I. Klimov, J. M. Katz, L. G. Rudenko
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	heterosubtypic immunity live influenza vaccine avian influenza viruses Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/4806dd9c405f4c75a204e5757654a6b3
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spelling	oai:doaj.org-article:4806dd9c405f4c75a204e5757654a6b32021-11-18T08:03:43ZHETEROSUBTYPIC IMMUNE RESPONSE AND CROSS-PROTECTION AGAINST A HIGHLY PATHOGENIC A (H5N1) INFLUENZA VIRUS IN MICE IMMUNIZED WITH COLD-ADAPTED A/LENINGRAD/134/17/57 (H2N2) INFLUENZA VIRUS1563-06252313-741X10.15789/1563-0625-2005-5-6-503-510https://doaj.org/article/4806dd9c405f4c75a204e5757654a6b32014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/598https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XWhile investigating the efficacy of an H5N2 ca reassortant vaccine candidate in protecting against a lethal challenge with a highly pathogenic (HP) H5N1 virus in the mouse model, we observed a degree of cross-protection provided by the ca Len/17 (H2N2) virus itself. 80% of mice administered a high dose of attenuated Len/17 vaccine intranasally (i.n.) survived after a lethal challenge with A/Hong Kong/483/97 H5N1 virus. Therefore, we investigated the basis of the cross- reactive immunity between H2N2 and H5N1 viruses that may have contributed to recovery from lethal HK/ 483 virus infection. Sera from mice immunized i.n. with Len/17 did not cross-react with HK/483 virus in neutralization or hemagglutination-inhibition assays, however IgG and IgA antibodies that cross-reacted with the hemagglutinin and neuraminidase of H5N1 1997 viruses were detected. Spleen cells from mice immunized i.n. with Len/17 vaccine showed enhanced production of IL-2, IL-4, IL-5, IL-10, and IFNγ following in vitro stimulation with inactivated H5N1 virus. Our findings indicate that both cross-reactive humoral and cellular immunity induced by Len/17 H2N2 vaccine may plays a role in recovery from lethal H5N1 virus infection. A better understanding of the mechanisms of heterosubtypic immunity will improve vaccine design against HP avian influenza viruses.A. R. RekstinJ. A. DeshevaX. LuG. I. AlexandrovaA. I. KlimovJ. M. KatzL. G. RudenkoSPb RAACIarticleheterosubtypic immunitylive influenza vaccineavian influenza virusesImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 7, Iss 5-6, Pp 503-510 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	heterosubtypic immunity live influenza vaccine avian influenza viruses Immunologic diseases. Allergy RC581-607
spellingShingle	heterosubtypic immunity live influenza vaccine avian influenza viruses Immunologic diseases. Allergy RC581-607 A. R. Rekstin J. A. Desheva X. Lu G. I. Alexandrova A. I. Klimov J. M. Katz L. G. Rudenko HETEROSUBTYPIC IMMUNE RESPONSE AND CROSS-PROTECTION AGAINST A HIGHLY PATHOGENIC A (H5N1) INFLUENZA VIRUS IN MICE IMMUNIZED WITH COLD-ADAPTED A/LENINGRAD/134/17/57 (H2N2) INFLUENZA VIRUS
description	While investigating the efficacy of an H5N2 ca reassortant vaccine candidate in protecting against a lethal challenge with a highly pathogenic (HP) H5N1 virus in the mouse model, we observed a degree of cross-protection provided by the ca Len/17 (H2N2) virus itself. 80% of mice administered a high dose of attenuated Len/17 vaccine intranasally (i.n.) survived after a lethal challenge with A/Hong Kong/483/97 H5N1 virus. Therefore, we investigated the basis of the cross- reactive immunity between H2N2 and H5N1 viruses that may have contributed to recovery from lethal HK/ 483 virus infection. Sera from mice immunized i.n. with Len/17 did not cross-react with HK/483 virus in neutralization or hemagglutination-inhibition assays, however IgG and IgA antibodies that cross-reacted with the hemagglutinin and neuraminidase of H5N1 1997 viruses were detected. Spleen cells from mice immunized i.n. with Len/17 vaccine showed enhanced production of IL-2, IL-4, IL-5, IL-10, and IFNγ following in vitro stimulation with inactivated H5N1 virus. Our findings indicate that both cross-reactive humoral and cellular immunity induced by Len/17 H2N2 vaccine may plays a role in recovery from lethal H5N1 virus infection. A better understanding of the mechanisms of heterosubtypic immunity will improve vaccine design against HP avian influenza viruses.
format	article
author	A. R. Rekstin J. A. Desheva X. Lu G. I. Alexandrova A. I. Klimov J. M. Katz L. G. Rudenko
author_facet	A. R. Rekstin J. A. Desheva X. Lu G. I. Alexandrova A. I. Klimov J. M. Katz L. G. Rudenko
author_sort	A. R. Rekstin
title	HETEROSUBTYPIC IMMUNE RESPONSE AND CROSS-PROTECTION AGAINST A HIGHLY PATHOGENIC A (H5N1) INFLUENZA VIRUS IN MICE IMMUNIZED WITH COLD-ADAPTED A/LENINGRAD/134/17/57 (H2N2) INFLUENZA VIRUS
title_short	HETEROSUBTYPIC IMMUNE RESPONSE AND CROSS-PROTECTION AGAINST A HIGHLY PATHOGENIC A (H5N1) INFLUENZA VIRUS IN MICE IMMUNIZED WITH COLD-ADAPTED A/LENINGRAD/134/17/57 (H2N2) INFLUENZA VIRUS
title_full	HETEROSUBTYPIC IMMUNE RESPONSE AND CROSS-PROTECTION AGAINST A HIGHLY PATHOGENIC A (H5N1) INFLUENZA VIRUS IN MICE IMMUNIZED WITH COLD-ADAPTED A/LENINGRAD/134/17/57 (H2N2) INFLUENZA VIRUS
title_fullStr	HETEROSUBTYPIC IMMUNE RESPONSE AND CROSS-PROTECTION AGAINST A HIGHLY PATHOGENIC A (H5N1) INFLUENZA VIRUS IN MICE IMMUNIZED WITH COLD-ADAPTED A/LENINGRAD/134/17/57 (H2N2) INFLUENZA VIRUS
title_full_unstemmed	HETEROSUBTYPIC IMMUNE RESPONSE AND CROSS-PROTECTION AGAINST A HIGHLY PATHOGENIC A (H5N1) INFLUENZA VIRUS IN MICE IMMUNIZED WITH COLD-ADAPTED A/LENINGRAD/134/17/57 (H2N2) INFLUENZA VIRUS
title_sort	heterosubtypic immune response and cross-protection against a highly pathogenic a (h5n1) influenza virus in mice immunized with cold-adapted a/leningrad/134/17/57 (h2n2) influenza virus
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/4806dd9c405f4c75a204e5757654a6b3
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HETEROSUBTYPIC IMMUNE RESPONSE AND CROSS-PROTECTION AGAINST A HIGHLY PATHOGENIC A (H5N1) INFLUENZA VIRUS IN MICE IMMUNIZED WITH COLD-ADAPTED A/LENINGRAD/134/17/57 (H2N2) INFLUENZA VIRUS

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