Strategic approach of multifaceted antibacterial mechanism of limonene traced in Escherichia coli

Abstract Antibacterial potential of Limonene against Multi Drug Resistant (MDR) pathogens was studied and mechanism explored. Microscopic techniques viz. Fluorescent Microscopy (FM), Scanning Electron Microscopy (SEM), and Transmission Electron Microscopy (TEM) indicated membrane disruption, cellula...

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Autores principales:	Akshi Gupta, Ebenezer Jeyakumar, Rubina Lawrence
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4809f52c41af49459d3147f8ff506067
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spelling	oai:doaj.org-article:4809f52c41af49459d3147f8ff5060672021-12-02T15:23:17ZStrategic approach of multifaceted antibacterial mechanism of limonene traced in Escherichia coli10.1038/s41598-021-92843-32045-2322https://doaj.org/article/4809f52c41af49459d3147f8ff5060672021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92843-3https://doaj.org/toc/2045-2322Abstract Antibacterial potential of Limonene against Multi Drug Resistant (MDR) pathogens was studied and mechanism explored. Microscopic techniques viz. Fluorescent Microscopy (FM), Scanning Electron Microscopy (SEM), and Transmission Electron Microscopy (TEM) indicated membrane disruption, cellular leakage and cell death of Escherichia coli (E. coli) cells when treated with limonene. Leakage of intracellular proteins, lipids and nucleic acid confirmed membrane damage and disruption of cell permeability barrier. Further, release of intracellular ATP, also suggested disruption of membrane barrier. Interaction of limonene with DNA revealed its capability in unwinding of plasmid, which could eventually inhibit DNA transcription and translation. Differential expression of various proteins and enzymes involved in transport, respiration, metabolism, chemotaxis, protein synthesis confirmed the mechanistic role of limonene on their functions. Limonene thus can be a potential candidate in drug development.Akshi GuptaEbenezer JeyakumarRubina LawrenceNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Akshi Gupta Ebenezer Jeyakumar Rubina Lawrence Strategic approach of multifaceted antibacterial mechanism of limonene traced in Escherichia coli
description	Abstract Antibacterial potential of Limonene against Multi Drug Resistant (MDR) pathogens was studied and mechanism explored. Microscopic techniques viz. Fluorescent Microscopy (FM), Scanning Electron Microscopy (SEM), and Transmission Electron Microscopy (TEM) indicated membrane disruption, cellular leakage and cell death of Escherichia coli (E. coli) cells when treated with limonene. Leakage of intracellular proteins, lipids and nucleic acid confirmed membrane damage and disruption of cell permeability barrier. Further, release of intracellular ATP, also suggested disruption of membrane barrier. Interaction of limonene with DNA revealed its capability in unwinding of plasmid, which could eventually inhibit DNA transcription and translation. Differential expression of various proteins and enzymes involved in transport, respiration, metabolism, chemotaxis, protein synthesis confirmed the mechanistic role of limonene on their functions. Limonene thus can be a potential candidate in drug development.
format	article
author	Akshi Gupta Ebenezer Jeyakumar Rubina Lawrence
author_facet	Akshi Gupta Ebenezer Jeyakumar Rubina Lawrence
author_sort	Akshi Gupta
title	Strategic approach of multifaceted antibacterial mechanism of limonene traced in Escherichia coli
title_short	Strategic approach of multifaceted antibacterial mechanism of limonene traced in Escherichia coli
title_full	Strategic approach of multifaceted antibacterial mechanism of limonene traced in Escherichia coli
title_fullStr	Strategic approach of multifaceted antibacterial mechanism of limonene traced in Escherichia coli
title_full_unstemmed	Strategic approach of multifaceted antibacterial mechanism of limonene traced in Escherichia coli
title_sort	strategic approach of multifaceted antibacterial mechanism of limonene traced in escherichia coli
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4809f52c41af49459d3147f8ff506067
work_keys_str_mv	AT akshigupta strategicapproachofmultifacetedantibacterialmechanismoflimonenetracedinescherichiacoli AT ebenezerjeyakumar strategicapproachofmultifacetedantibacterialmechanismoflimonenetracedinescherichiacoli AT rubinalawrence strategicapproachofmultifacetedantibacterialmechanismoflimonenetracedinescherichiacoli
_version_	1718387261150593024

Strategic approach of multifaceted antibacterial mechanism of limonene traced in Escherichia coli

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