Fish oil supplementation associated with decreased cellular degeneration and increased cellular proliferation 6 weeks after middle cerebral artery occlusion in the rat

Michaela C Pascoe,1 David W Howells, 2David P Crewther,1 Leeanne M Carey,2,3 Sheila G Crewther4 1Brain Sciences Institute, Swinburne University, ²Florey Institute of Neuroscience and Mental Health, University of Melbourne, 3Department of Occupational Therapy, School of Allied Hea...

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Autores principales:	Pascoe MC, Howells DW, Crewther DP, Carey LM, Crewther SG
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
Acceso en línea:	https://doaj.org/article/480aca1e61da41fe8a94a0bdcd9cdcb6
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spelling	oai:doaj.org-article:480aca1e61da41fe8a94a0bdcd9cdcb62021-12-02T06:03:34ZFish oil supplementation associated with decreased cellular degeneration and increased cellular proliferation 6 weeks after middle cerebral artery occlusion in the rat1178-2021https://doaj.org/article/480aca1e61da41fe8a94a0bdcd9cdcb62015-01-01T00:00:00Zhttp://www.dovepress.com/fish-oil-supplementation-associated-with-decreased-cellular-degenerati-peer-reviewed-article-NDThttps://doaj.org/toc/1178-2021 Michaela C Pascoe,1 David W Howells, 2David P Crewther,1 Leeanne M Carey,2,3 Sheila G Crewther4 1Brain Sciences Institute, Swinburne University, ²Florey Institute of Neuroscience and Mental Health, University of Melbourne, 3Department of Occupational Therapy, School of Allied Health La Trobe University, 4School of Psychological Science, La Trobe University, Melbourne, VIC, Australia Abstract: Anti-inflammatory long-chain omega-3 polyunsaturated fatty acids (n-3-LC-PUFAs) are both neuroprotective and have antidepressive effects. However the influence of dietary supplemented n-3-LC-PUFAs on inflammation-related cell death and proliferation after middle cerebral artery occlusion (MCAo)-induced stroke is unknown. We have previously demonstrated that anxiety-like and hyperactive locomotor behaviors are reduced in n-3-LC-PUFA-fed MCAo animals. Thus in the present study, male hooded Wistar rats were exposed to MCAo or sham surgeries and examined behaviorally 6 weeks later, prior to euthanasia and examination of lesion size, cell death and proliferation in the dentate gyrus, cornu ammonis region of the hippocampus of the ipsilesional hemispheres, and the thalamus of the ipsilesional and contralesional hemispheres. Markers of cell genesis and cell degeneration in the hippocampus or thalamus of the ipsilesional hemisphere did not differ between surgery and diet groups 6 weeks post MCAo. Dietary supplementation with n-3-LC-PUFA decreased cell degeneration and increased cell proliferation in the thalamic region of the contralesional hemisphere. MCAo–associated cell degeneration in the hippocampus and thalamus positively correlated with anxiety-like and hyperactive locomotor behaviors previously reported in these animals. These results suggest that anti-inflammatory n-3-LC-PUFA supplementation appears to have cellular protective effects after MCAo in the rat, which may affect behavioral outcomes. Keywords: apoptosis, polyunsaturated fatty acids, contralesional hemisphere, stroke, anxiety, depressionPascoe MCHowells DWCrewther DPCarey LMCrewther SGDove Medical PressarticleNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571Neurology. Diseases of the nervous systemRC346-429ENNeuropsychiatric Disease and Treatment, Vol 2015, Iss default, Pp 153-164 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
spellingShingle	Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429 Pascoe MC Howells DW Crewther DP Carey LM Crewther SG Fish oil supplementation associated with decreased cellular degeneration and increased cellular proliferation 6 weeks after middle cerebral artery occlusion in the rat
description	Michaela C Pascoe,1 David W Howells, 2David P Crewther,1 Leeanne M Carey,2,3 Sheila G Crewther4 1Brain Sciences Institute, Swinburne University, ²Florey Institute of Neuroscience and Mental Health, University of Melbourne, 3Department of Occupational Therapy, School of Allied Health La Trobe University, 4School of Psychological Science, La Trobe University, Melbourne, VIC, Australia Abstract: Anti-inflammatory long-chain omega-3 polyunsaturated fatty acids (n-3-LC-PUFAs) are both neuroprotective and have antidepressive effects. However the influence of dietary supplemented n-3-LC-PUFAs on inflammation-related cell death and proliferation after middle cerebral artery occlusion (MCAo)-induced stroke is unknown. We have previously demonstrated that anxiety-like and hyperactive locomotor behaviors are reduced in n-3-LC-PUFA-fed MCAo animals. Thus in the present study, male hooded Wistar rats were exposed to MCAo or sham surgeries and examined behaviorally 6 weeks later, prior to euthanasia and examination of lesion size, cell death and proliferation in the dentate gyrus, cornu ammonis region of the hippocampus of the ipsilesional hemispheres, and the thalamus of the ipsilesional and contralesional hemispheres. Markers of cell genesis and cell degeneration in the hippocampus or thalamus of the ipsilesional hemisphere did not differ between surgery and diet groups 6 weeks post MCAo. Dietary supplementation with n-3-LC-PUFA decreased cell degeneration and increased cell proliferation in the thalamic region of the contralesional hemisphere. MCAo–associated cell degeneration in the hippocampus and thalamus positively correlated with anxiety-like and hyperactive locomotor behaviors previously reported in these animals. These results suggest that anti-inflammatory n-3-LC-PUFA supplementation appears to have cellular protective effects after MCAo in the rat, which may affect behavioral outcomes. Keywords: apoptosis, polyunsaturated fatty acids, contralesional hemisphere, stroke, anxiety, depression
format	article
author	Pascoe MC Howells DW Crewther DP Carey LM Crewther SG
author_facet	Pascoe MC Howells DW Crewther DP Carey LM Crewther SG
author_sort	Pascoe MC
title	Fish oil supplementation associated with decreased cellular degeneration and increased cellular proliferation 6 weeks after middle cerebral artery occlusion in the rat
title_short	Fish oil supplementation associated with decreased cellular degeneration and increased cellular proliferation 6 weeks after middle cerebral artery occlusion in the rat
title_full	Fish oil supplementation associated with decreased cellular degeneration and increased cellular proliferation 6 weeks after middle cerebral artery occlusion in the rat
title_fullStr	Fish oil supplementation associated with decreased cellular degeneration and increased cellular proliferation 6 weeks after middle cerebral artery occlusion in the rat
title_full_unstemmed	Fish oil supplementation associated with decreased cellular degeneration and increased cellular proliferation 6 weeks after middle cerebral artery occlusion in the rat
title_sort	fish oil supplementation associated with decreased cellular degeneration and increased cellular proliferation 6 weeks after middle cerebral artery occlusion in the rat
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/480aca1e61da41fe8a94a0bdcd9cdcb6
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Fish oil supplementation associated with decreased cellular degeneration and increased cellular proliferation 6 weeks after middle cerebral artery occlusion in the rat

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