Impact of short-term storage of plasma samples on quantitation of ultra-low levels of interleukin-6

Objective To quantitate plasma interleukin-6 (IL-6) levels in healthy individuals and to clarify how these levels are affected by blood sample handling procedures during short-term storage. Methods Ethylenediaminetetraacetic acid (EDTA)-treated plasma samples were simultaneously collected from 14 he...

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Autores principales:	Masaki Yamaguchi, Tomonobu Koizumi, Mitsutoshi Sugano
Formato:	article
Lenguaje:	EN
Publicado:	SAGE Publishing 2021
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/480ed6ae9c03490083266c57bf09de82
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spelling	oai:doaj.org-article:480ed6ae9c03490083266c57bf09de822021-11-15T00:03:43ZImpact of short-term storage of plasma samples on quantitation of ultra-low levels of interleukin-61473-230010.1177/03000605211056846https://doaj.org/article/480ed6ae9c03490083266c57bf09de822021-11-01T00:00:00Zhttps://doi.org/10.1177/03000605211056846https://doaj.org/toc/1473-2300Objective To quantitate plasma interleukin-6 (IL-6) levels in healthy individuals and to clarify how these levels are affected by blood sample handling procedures during short-term storage. Methods Ethylenediaminetetraacetic acid (EDTA)-treated plasma samples were simultaneously collected from 14 healthy individuals and stored on ice prior to analysis of the IL-6 levels. White blood cells (WBCs), red blood cells, and platelets were counted immediately after blood collection. IL-6 levels were analyzed every 30 minutes using a commercial electrochemiluminescence immunoassay. Results Correlation coefficients between plasma IL-6 levels and WBC counts ranged between 0.605 and 0.554, higher than those for other cell types. The lowest IL-6 value in healthy individuals was estimated at 0.04 pg/mL and the mean values remained under 2 pg/mL over time. Conclusion Analysis of IL-6 levels in EDTA-treated plasma samples centrifuged within 1 hour and stored on ice can be performed within 90 minutes of short-term storage if the analytical method has a sensitivity in the range of 10 fg/mL.Masaki YamaguchiTomonobu KoizumiMitsutoshi SuganoSAGE PublishingarticleMedicine (General)R5-920ENJournal of International Medical Research, Vol 49 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Masaki Yamaguchi Tomonobu Koizumi Mitsutoshi Sugano Impact of short-term storage of plasma samples on quantitation of ultra-low levels of interleukin-6
description	Objective To quantitate plasma interleukin-6 (IL-6) levels in healthy individuals and to clarify how these levels are affected by blood sample handling procedures during short-term storage. Methods Ethylenediaminetetraacetic acid (EDTA)-treated plasma samples were simultaneously collected from 14 healthy individuals and stored on ice prior to analysis of the IL-6 levels. White blood cells (WBCs), red blood cells, and platelets were counted immediately after blood collection. IL-6 levels were analyzed every 30 minutes using a commercial electrochemiluminescence immunoassay. Results Correlation coefficients between plasma IL-6 levels and WBC counts ranged between 0.605 and 0.554, higher than those for other cell types. The lowest IL-6 value in healthy individuals was estimated at 0.04 pg/mL and the mean values remained under 2 pg/mL over time. Conclusion Analysis of IL-6 levels in EDTA-treated plasma samples centrifuged within 1 hour and stored on ice can be performed within 90 minutes of short-term storage if the analytical method has a sensitivity in the range of 10 fg/mL.
format	article
author	Masaki Yamaguchi Tomonobu Koizumi Mitsutoshi Sugano
author_facet	Masaki Yamaguchi Tomonobu Koizumi Mitsutoshi Sugano
author_sort	Masaki Yamaguchi
title	Impact of short-term storage of plasma samples on quantitation of ultra-low levels of interleukin-6
title_short	Impact of short-term storage of plasma samples on quantitation of ultra-low levels of interleukin-6
title_full	Impact of short-term storage of plasma samples on quantitation of ultra-low levels of interleukin-6
title_fullStr	Impact of short-term storage of plasma samples on quantitation of ultra-low levels of interleukin-6
title_full_unstemmed	Impact of short-term storage of plasma samples on quantitation of ultra-low levels of interleukin-6
title_sort	impact of short-term storage of plasma samples on quantitation of ultra-low levels of interleukin-6
publisher	SAGE Publishing
publishDate	2021
url	https://doaj.org/article/480ed6ae9c03490083266c57bf09de82
work_keys_str_mv	AT masakiyamaguchi impactofshorttermstorageofplasmasamplesonquantitationofultralowlevelsofinterleukin6 AT tomonobukoizumi impactofshorttermstorageofplasmasamplesonquantitationofultralowlevelsofinterleukin6 AT mitsutoshisugano impactofshorttermstorageofplasmasamplesonquantitationofultralowlevelsofinterleukin6
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Impact of short-term storage of plasma samples on quantitation of ultra-low levels of interleukin-6

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