Synaptotagmin-13 Is a Neuroendocrine Marker in Brain, Intestine and Pancreas

Synaptotagmin-13 (Syt13) is an atypical member of the vesicle trafficking synaptotagmin protein family. The expression pattern and the biological function of this Ca<sup>2+</sup>-independent protein are not well resolved. Here, we have generated a novel Syt13-Venus fusion (Syt13-VF) fluo...

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Autores principales:	Marta Tarquis-Medina, Katharina Scheibner, Ismael González-García, Aimée Bastidas-Ponce, Michael Sterr, Jessica Jaki, Silvia Schirge, Cristina García-Cáceres, Heiko Lickert, Mostafa Bakhti
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	synaptotagmin-13 Syt13 fluorescent reporter endocrine lineage brain intestine Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/4823f1a594184ccd8b7d615cfc60b619
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spelling	oai:doaj.org-article:4823f1a594184ccd8b7d615cfc60b6192021-11-25T17:57:32ZSynaptotagmin-13 Is a Neuroendocrine Marker in Brain, Intestine and Pancreas10.3390/ijms2222125261422-00671661-6596https://doaj.org/article/4823f1a594184ccd8b7d615cfc60b6192021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12526https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Synaptotagmin-13 (Syt13) is an atypical member of the vesicle trafficking synaptotagmin protein family. The expression pattern and the biological function of this Ca<sup>2+</sup>-independent protein are not well resolved. Here, we have generated a novel Syt13-Venus fusion (Syt13-VF) fluorescence reporter allele to track and isolate tissues and cells expressing Syt13 protein. The reporter allele is regulated by endogenous cis-regulatory elements of <i>Syt13</i> and the fusion protein follows an identical expression pattern of the endogenous Syt13 protein. The homozygous reporter mice are viable and fertile. We identify the expression of the Syt13-VF reporter in different regions of the brain with high expression in tyrosine hydroxylase (TH)-expressing and oxytocin-producing neuroendocrine cells. Moreover, Syt13-VF is highly restricted to all enteroendocrine cells in the adult intestine that can be traced in live imaging. Finally, Syt13-VF protein is expressed in the pancreatic endocrine lineage, allowing their specific isolation by flow sorting. These findings demonstrate high expression levels of Syt13 in the endocrine lineages in three major organs harboring these secretory cells. Collectively, the Syt13-VF reporter mouse line provides a unique and reliable tool to dissect the spatio-temporal expression pattern of Syt13 and enables isolation of Syt13-expressing cells that will aid in deciphering the molecular functions of this protein in the neuroendocrine system.Marta Tarquis-MedinaKatharina ScheibnerIsmael González-GarcíaAimée Bastidas-PonceMichael SterrJessica JakiSilvia SchirgeCristina García-CáceresHeiko LickertMostafa BakhtiMDPI AGarticlesynaptotagmin-13Syt13fluorescent reporterendocrine lineagebrainintestineBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12526, p 12526 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	synaptotagmin-13 Syt13 fluorescent reporter endocrine lineage brain intestine Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	synaptotagmin-13 Syt13 fluorescent reporter endocrine lineage brain intestine Biology (General) QH301-705.5 Chemistry QD1-999 Marta Tarquis-Medina Katharina Scheibner Ismael González-García Aimée Bastidas-Ponce Michael Sterr Jessica Jaki Silvia Schirge Cristina García-Cáceres Heiko Lickert Mostafa Bakhti Synaptotagmin-13 Is a Neuroendocrine Marker in Brain, Intestine and Pancreas
description	Synaptotagmin-13 (Syt13) is an atypical member of the vesicle trafficking synaptotagmin protein family. The expression pattern and the biological function of this Ca<sup>2+</sup>-independent protein are not well resolved. Here, we have generated a novel Syt13-Venus fusion (Syt13-VF) fluorescence reporter allele to track and isolate tissues and cells expressing Syt13 protein. The reporter allele is regulated by endogenous cis-regulatory elements of <i>Syt13</i> and the fusion protein follows an identical expression pattern of the endogenous Syt13 protein. The homozygous reporter mice are viable and fertile. We identify the expression of the Syt13-VF reporter in different regions of the brain with high expression in tyrosine hydroxylase (TH)-expressing and oxytocin-producing neuroendocrine cells. Moreover, Syt13-VF is highly restricted to all enteroendocrine cells in the adult intestine that can be traced in live imaging. Finally, Syt13-VF protein is expressed in the pancreatic endocrine lineage, allowing their specific isolation by flow sorting. These findings demonstrate high expression levels of Syt13 in the endocrine lineages in three major organs harboring these secretory cells. Collectively, the Syt13-VF reporter mouse line provides a unique and reliable tool to dissect the spatio-temporal expression pattern of Syt13 and enables isolation of Syt13-expressing cells that will aid in deciphering the molecular functions of this protein in the neuroendocrine system.
format	article
author	Marta Tarquis-Medina Katharina Scheibner Ismael González-García Aimée Bastidas-Ponce Michael Sterr Jessica Jaki Silvia Schirge Cristina García-Cáceres Heiko Lickert Mostafa Bakhti
author_facet	Marta Tarquis-Medina Katharina Scheibner Ismael González-García Aimée Bastidas-Ponce Michael Sterr Jessica Jaki Silvia Schirge Cristina García-Cáceres Heiko Lickert Mostafa Bakhti
author_sort	Marta Tarquis-Medina
title	Synaptotagmin-13 Is a Neuroendocrine Marker in Brain, Intestine and Pancreas
title_short	Synaptotagmin-13 Is a Neuroendocrine Marker in Brain, Intestine and Pancreas
title_full	Synaptotagmin-13 Is a Neuroendocrine Marker in Brain, Intestine and Pancreas
title_fullStr	Synaptotagmin-13 Is a Neuroendocrine Marker in Brain, Intestine and Pancreas
title_full_unstemmed	Synaptotagmin-13 Is a Neuroendocrine Marker in Brain, Intestine and Pancreas
title_sort	synaptotagmin-13 is a neuroendocrine marker in brain, intestine and pancreas
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/4823f1a594184ccd8b7d615cfc60b619
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Synaptotagmin-13 Is a Neuroendocrine Marker in Brain, Intestine and Pancreas

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