Ciliates (Alveolata, Ciliophora) as bioindicators of environmental pressure: A karstic river case

Ciliates are single celled eukaryotes recognized as key players in the microbial loop of aquatic ecosystems. The present study was carried out on the Krka River (Croatia), a karst freshwater ecosystem characterized by tufa barriers, biomineralization and highly diverse aquatic communities. The main...

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Autores principales:	Antonija Kulaš, Vesna Gulin, Renata Matoničkin Kepčija, Petar Žutinić, Mirela Sertić Perić, Sandi Orlić, Katarina Kajan, Thorsten Stoeck, Guillaume Lentendu, Ivan Čanjevac, Ivan Martinić, Marija Gligora Udovič
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Ciliates Morphology eDNA metabarcoding Bioindicators Molecular OTUs Biofilms Ecology QH540-549.5
Acceso en línea:	https://doaj.org/article/482643e5373f4de2818c7a9a82ec48b2
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spelling	oai:doaj.org-article:482643e5373f4de2818c7a9a82ec48b22021-12-01T04:46:00ZCiliates (Alveolata, Ciliophora) as bioindicators of environmental pressure: A karstic river case1470-160X10.1016/j.ecolind.2021.107430https://doaj.org/article/482643e5373f4de2818c7a9a82ec48b22021-05-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1470160X21000959https://doaj.org/toc/1470-160XCiliates are single celled eukaryotes recognized as key players in the microbial loop of aquatic ecosystems. The present study was carried out on the Krka River (Croatia), a karst freshwater ecosystem characterized by tufa barriers, biomineralization and highly diverse aquatic communities. The main aims of the study were to investigate ciliate community structure in the biofilm (i.e. periphyton) samples collected from light- and dark-exposed lithified tufa/stones. Furthermore, by establishing links between ciliate community patterns and environmental parameters, we aimed to assess the bioindicator potential of specific ciliate taxa for environmental monitoring of freshwater habitats. The periphyton sampling was performed at four representative sites of the river source, upstream, middle and downstream river sections. Ciliate community was investigated via traditional microscopy analyses and environmental DNA (eDNA) metabarcoding (Illumina sequencing of the hypervariable V9-region of the SSU rRNA gene). The molecular approach recorded a substantially higher number of ciliate taxa, most of which taxonomically belonging to genera typically occurring in tufa barriers. The results from microscopy analyses did not show any links between ciliate community structure and sampling location. However, eDNA approach indicated significant differences among the sampling locations regarding the ciliate community structure. Thereby, hydrological parameters and saprobiological classification of the sampling sites were the main structuring factors for ciliate community. The coupling of eDNA metabarcoding with the morphological approach provides a robust, in-depth analytical system in elucidating the bioindicator potential of ciliated protists.Antonija KulašVesna GulinRenata Matoničkin KepčijaPetar ŽutinićMirela Sertić PerićSandi OrlićKatarina KajanThorsten StoeckGuillaume LentenduIvan ČanjevacIvan MartinićMarija Gligora UdovičElsevierarticleCiliatesMorphologyeDNA metabarcodingBioindicatorsMolecular OTUsBiofilmsEcologyQH540-549.5ENEcological Indicators, Vol 124, Iss , Pp 107430- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Ciliates Morphology eDNA metabarcoding Bioindicators Molecular OTUs Biofilms Ecology QH540-549.5
spellingShingle	Ciliates Morphology eDNA metabarcoding Bioindicators Molecular OTUs Biofilms Ecology QH540-549.5 Antonija Kulaš Vesna Gulin Renata Matoničkin Kepčija Petar Žutinić Mirela Sertić Perić Sandi Orlić Katarina Kajan Thorsten Stoeck Guillaume Lentendu Ivan Čanjevac Ivan Martinić Marija Gligora Udovič Ciliates (Alveolata, Ciliophora) as bioindicators of environmental pressure: A karstic river case
description	Ciliates are single celled eukaryotes recognized as key players in the microbial loop of aquatic ecosystems. The present study was carried out on the Krka River (Croatia), a karst freshwater ecosystem characterized by tufa barriers, biomineralization and highly diverse aquatic communities. The main aims of the study were to investigate ciliate community structure in the biofilm (i.e. periphyton) samples collected from light- and dark-exposed lithified tufa/stones. Furthermore, by establishing links between ciliate community patterns and environmental parameters, we aimed to assess the bioindicator potential of specific ciliate taxa for environmental monitoring of freshwater habitats. The periphyton sampling was performed at four representative sites of the river source, upstream, middle and downstream river sections. Ciliate community was investigated via traditional microscopy analyses and environmental DNA (eDNA) metabarcoding (Illumina sequencing of the hypervariable V9-region of the SSU rRNA gene). The molecular approach recorded a substantially higher number of ciliate taxa, most of which taxonomically belonging to genera typically occurring in tufa barriers. The results from microscopy analyses did not show any links between ciliate community structure and sampling location. However, eDNA approach indicated significant differences among the sampling locations regarding the ciliate community structure. Thereby, hydrological parameters and saprobiological classification of the sampling sites were the main structuring factors for ciliate community. The coupling of eDNA metabarcoding with the morphological approach provides a robust, in-depth analytical system in elucidating the bioindicator potential of ciliated protists.
format	article
author	Antonija Kulaš Vesna Gulin Renata Matoničkin Kepčija Petar Žutinić Mirela Sertić Perić Sandi Orlić Katarina Kajan Thorsten Stoeck Guillaume Lentendu Ivan Čanjevac Ivan Martinić Marija Gligora Udovič
author_facet	Antonija Kulaš Vesna Gulin Renata Matoničkin Kepčija Petar Žutinić Mirela Sertić Perić Sandi Orlić Katarina Kajan Thorsten Stoeck Guillaume Lentendu Ivan Čanjevac Ivan Martinić Marija Gligora Udovič
author_sort	Antonija Kulaš
title	Ciliates (Alveolata, Ciliophora) as bioindicators of environmental pressure: A karstic river case
title_short	Ciliates (Alveolata, Ciliophora) as bioindicators of environmental pressure: A karstic river case
title_full	Ciliates (Alveolata, Ciliophora) as bioindicators of environmental pressure: A karstic river case
title_fullStr	Ciliates (Alveolata, Ciliophora) as bioindicators of environmental pressure: A karstic river case
title_full_unstemmed	Ciliates (Alveolata, Ciliophora) as bioindicators of environmental pressure: A karstic river case
title_sort	ciliates (alveolata, ciliophora) as bioindicators of environmental pressure: a karstic river case
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/482643e5373f4de2818c7a9a82ec48b2
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Ciliates (Alveolata, Ciliophora) as bioindicators of environmental pressure: A karstic river case

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