Genotypic Diversity of Ciprofloxacin Nonsusceptibility and Its Relationship with Minimum Inhibitory Concentrations in Nontyphoidal <i>Salmonella</i> Clinical Isolates in Taiwan

This study analyzed the genetic diversity of ciprofloxacin (CIP) nonsusceptibility and the relationship between two major mechanisms and minimum inhibitory concentrations (MICs) of CIP in nontyphoidal <i>Salmonella</i> (NTS). Chromosomal mutations in quinolone resistance-determining regi...

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Autores principales:	Shiuh-Bin Fang, Tsai-Ling Yang Lauderdale, Chih-Hung Huang, Pei-Ru Chang, Yuan-Hung Wang, Katsumi Shigemura, Ying-Hsiu Lin, Wei-Chiao Chang, Ke-Chuan Wang, Tzu-Wen Huang, Yu-Chu Chang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	ciprofloxacin nonsusceptibility minimum inhibitory concentrations quinolone resistance determining regions plasmid-mediated quinolone resistance nontyphoidal <i>Salmonella</i> Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/482d798b0eff490d8ccba72e55800cb0
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spelling	oai:doaj.org-article:482d798b0eff490d8ccba72e55800cb02021-11-25T16:24:25ZGenotypic Diversity of Ciprofloxacin Nonsusceptibility and Its Relationship with Minimum Inhibitory Concentrations in Nontyphoidal <i>Salmonella</i> Clinical Isolates in Taiwan10.3390/antibiotics101113832079-6382https://doaj.org/article/482d798b0eff490d8ccba72e55800cb02021-11-01T00:00:00Zhttps://www.mdpi.com/2079-6382/10/11/1383https://doaj.org/toc/2079-6382This study analyzed the genetic diversity of ciprofloxacin (CIP) nonsusceptibility and the relationship between two major mechanisms and minimum inhibitory concentrations (MICs) of CIP in nontyphoidal <i>Salmonella</i> (NTS). Chromosomal mutations in quinolone resistance-determining regions (QRDRs) and plasmid-mediated quinolone resistance (PMQR) genes were searched from ResFinder, ARG-ANNOT, and PubMed for designing the sequencing regions in <i>gyrA</i>, <i>gyrB</i>, <i>parC</i>, and <i>parE</i>, and the 13 polymerase chain reactions for PMQR genes. We found that QRDR mutations were detected in <i>gyrA</i> (82.1%), <i>parC</i> (59.0%), and <i>parE</i> (20.5%) but not in <i>gyrB</i> among the 39 isolates. Five of the 13 PMQR genes were identified, including <i>oqxA</i> (28.2%), <i>oqxB</i> (28.2%), <i>qnrS</i> (18.0%), <i>aac</i>(6′)-<i>Ib</i>-<i>cr</i> (10.3%), and <i>qnrB</i> (5.1%), which correlated with the MICs of CIP within 0.25–2 μg/mL, and it was found that <i>oxqAB</i> contributed more than <i>qnr</i> genes to increase the MICs. All the isolates contained either QRDR mutations (53.8%), PMQR genes (15.4%), or both (30.8%). QRDR mutations (84.6%) were more commonly detected than PMQR genes (46.2%). QRDR mutation numbers were significantly associated with MICs (<i>p</i> < 0.001). Double mutations in <i>gyrA</i> and <i>parC</i> determined high CIP resistance (MICs ≥ 4 μg/mL). PMQR genes contributed to intermediate to low CIP resistance (MICs 0.25–2 μg/mL), thus providing insights into mechanisms underlying CIP resistance.Shiuh-Bin FangTsai-Ling Yang LauderdaleChih-Hung HuangPei-Ru ChangYuan-Hung WangKatsumi ShigemuraYing-Hsiu LinWei-Chiao ChangKe-Chuan WangTzu-Wen HuangYu-Chu ChangMDPI AGarticleciprofloxacin nonsusceptibilityminimum inhibitory concentrationsquinolone resistance determining regionsplasmid-mediated quinolone resistancenontyphoidal <i>Salmonella</i>Therapeutics. PharmacologyRM1-950ENAntibiotics, Vol 10, Iss 1383, p 1383 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ciprofloxacin nonsusceptibility minimum inhibitory concentrations quinolone resistance determining regions plasmid-mediated quinolone resistance nontyphoidal <i>Salmonella</i> Therapeutics. Pharmacology RM1-950
spellingShingle	ciprofloxacin nonsusceptibility minimum inhibitory concentrations quinolone resistance determining regions plasmid-mediated quinolone resistance nontyphoidal <i>Salmonella</i> Therapeutics. Pharmacology RM1-950 Shiuh-Bin Fang Tsai-Ling Yang Lauderdale Chih-Hung Huang Pei-Ru Chang Yuan-Hung Wang Katsumi Shigemura Ying-Hsiu Lin Wei-Chiao Chang Ke-Chuan Wang Tzu-Wen Huang Yu-Chu Chang Genotypic Diversity of Ciprofloxacin Nonsusceptibility and Its Relationship with Minimum Inhibitory Concentrations in Nontyphoidal <i>Salmonella</i> Clinical Isolates in Taiwan
description	This study analyzed the genetic diversity of ciprofloxacin (CIP) nonsusceptibility and the relationship between two major mechanisms and minimum inhibitory concentrations (MICs) of CIP in nontyphoidal <i>Salmonella</i> (NTS). Chromosomal mutations in quinolone resistance-determining regions (QRDRs) and plasmid-mediated quinolone resistance (PMQR) genes were searched from ResFinder, ARG-ANNOT, and PubMed for designing the sequencing regions in <i>gyrA</i>, <i>gyrB</i>, <i>parC</i>, and <i>parE</i>, and the 13 polymerase chain reactions for PMQR genes. We found that QRDR mutations were detected in <i>gyrA</i> (82.1%), <i>parC</i> (59.0%), and <i>parE</i> (20.5%) but not in <i>gyrB</i> among the 39 isolates. Five of the 13 PMQR genes were identified, including <i>oqxA</i> (28.2%), <i>oqxB</i> (28.2%), <i>qnrS</i> (18.0%), <i>aac</i>(6′)-<i>Ib</i>-<i>cr</i> (10.3%), and <i>qnrB</i> (5.1%), which correlated with the MICs of CIP within 0.25–2 μg/mL, and it was found that <i>oxqAB</i> contributed more than <i>qnr</i> genes to increase the MICs. All the isolates contained either QRDR mutations (53.8%), PMQR genes (15.4%), or both (30.8%). QRDR mutations (84.6%) were more commonly detected than PMQR genes (46.2%). QRDR mutation numbers were significantly associated with MICs (<i>p</i> < 0.001). Double mutations in <i>gyrA</i> and <i>parC</i> determined high CIP resistance (MICs ≥ 4 μg/mL). PMQR genes contributed to intermediate to low CIP resistance (MICs 0.25–2 μg/mL), thus providing insights into mechanisms underlying CIP resistance.
format	article
author	Shiuh-Bin Fang Tsai-Ling Yang Lauderdale Chih-Hung Huang Pei-Ru Chang Yuan-Hung Wang Katsumi Shigemura Ying-Hsiu Lin Wei-Chiao Chang Ke-Chuan Wang Tzu-Wen Huang Yu-Chu Chang
author_facet	Shiuh-Bin Fang Tsai-Ling Yang Lauderdale Chih-Hung Huang Pei-Ru Chang Yuan-Hung Wang Katsumi Shigemura Ying-Hsiu Lin Wei-Chiao Chang Ke-Chuan Wang Tzu-Wen Huang Yu-Chu Chang
author_sort	Shiuh-Bin Fang
title	Genotypic Diversity of Ciprofloxacin Nonsusceptibility and Its Relationship with Minimum Inhibitory Concentrations in Nontyphoidal <i>Salmonella</i> Clinical Isolates in Taiwan
title_short	Genotypic Diversity of Ciprofloxacin Nonsusceptibility and Its Relationship with Minimum Inhibitory Concentrations in Nontyphoidal <i>Salmonella</i> Clinical Isolates in Taiwan
title_full	Genotypic Diversity of Ciprofloxacin Nonsusceptibility and Its Relationship with Minimum Inhibitory Concentrations in Nontyphoidal <i>Salmonella</i> Clinical Isolates in Taiwan
title_fullStr	Genotypic Diversity of Ciprofloxacin Nonsusceptibility and Its Relationship with Minimum Inhibitory Concentrations in Nontyphoidal <i>Salmonella</i> Clinical Isolates in Taiwan
title_full_unstemmed	Genotypic Diversity of Ciprofloxacin Nonsusceptibility and Its Relationship with Minimum Inhibitory Concentrations in Nontyphoidal <i>Salmonella</i> Clinical Isolates in Taiwan
title_sort	genotypic diversity of ciprofloxacin nonsusceptibility and its relationship with minimum inhibitory concentrations in nontyphoidal <i>salmonella</i> clinical isolates in taiwan
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/482d798b0eff490d8ccba72e55800cb0
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Genotypic Diversity of Ciprofloxacin Nonsusceptibility and Its Relationship with Minimum Inhibitory Concentrations in Nontyphoidal <i>Salmonella</i> Clinical Isolates in Taiwan

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