Typing of Indigenous Campylobacter spp. From Serbia by M-PCR and RAPD

Campylobacteriosis is an infectious human disease caused by thermophilic Campylobacter species, mainly C. jejuni and C. coli. It is the leading cause of human gastroenteritis today with the number of cases surpassing the number of Salmonella poisoning cases. The epidemiology of the agent is not comp...

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Autores principales:	Jošić Dragana, Petković Jelena, Bunčić Olivera, Lepšanović Zorica, Pivić Radmila, Rašić Zoran, Katić Vera
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2016
Materias:	broilers campylobacter spp. epidemiology multiplex-pcr rapd Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/482d81c400eb402aab5863a96961d434
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spelling	oai:doaj.org-article:482d81c400eb402aab5863a96961d4342021-11-17T21:27:51ZTyping of Indigenous Campylobacter spp. From Serbia by M-PCR and RAPD1820-744810.1515/acve-2016-0017https://doaj.org/article/482d81c400eb402aab5863a96961d4342016-06-01T00:00:00Zhttps://doi.org/10.1515/acve-2016-0017https://doaj.org/toc/1820-7448Campylobacteriosis is an infectious human disease caused by thermophilic Campylobacter species, mainly C. jejuni and C. coli. It is the leading cause of human gastroenteritis today with the number of cases surpassing the number of Salmonella poisoning cases. The epidemiology of the agent is not completely clear, but a number of investigations indicate an important role of broiler meat in human infections. The purpose of this study was to investigate the diversity of Campylobacter jejuni strains present in the Republic of Serbia and to determine a fast and reliable system for the confirmation and typing of the isolated strains. Samples taken at slaughterhouses, broiler farms, as well as two human isolates of Campylobacter species have been investigated. Strain identification was performed by multiplex-PCR. Genotyping was performed by Random Amplified Polymorphic DNA (RAPD) with multiple primers. Using several unusual primers and a newly designed one (DJP17), we report on the RAPD types of indigenous Campylobacter species. RAPD profiles showed different levels of discrimination between the isolates, depending on the primer: SPH1 and AG15 were informative only in part and better results were obtained with AP10, AK16 and DJP17.Jošić DraganaPetković JelenaBunčić OliveraLepšanović ZoricaPivić RadmilaRašić ZoranKatić VeraSciendoarticlebroilerscampylobacter spp.epidemiologymultiplex-pcrrapdVeterinary medicineSF600-1100ENActa Veterinaria, Vol 66, Iss 2, Pp 203-213 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	broilers campylobacter spp. epidemiology multiplex-pcr rapd Veterinary medicine SF600-1100
spellingShingle	broilers campylobacter spp. epidemiology multiplex-pcr rapd Veterinary medicine SF600-1100 Jošić Dragana Petković Jelena Bunčić Olivera Lepšanović Zorica Pivić Radmila Rašić Zoran Katić Vera Typing of Indigenous Campylobacter spp. From Serbia by M-PCR and RAPD
description	Campylobacteriosis is an infectious human disease caused by thermophilic Campylobacter species, mainly C. jejuni and C. coli. It is the leading cause of human gastroenteritis today with the number of cases surpassing the number of Salmonella poisoning cases. The epidemiology of the agent is not completely clear, but a number of investigations indicate an important role of broiler meat in human infections. The purpose of this study was to investigate the diversity of Campylobacter jejuni strains present in the Republic of Serbia and to determine a fast and reliable system for the confirmation and typing of the isolated strains. Samples taken at slaughterhouses, broiler farms, as well as two human isolates of Campylobacter species have been investigated. Strain identification was performed by multiplex-PCR. Genotyping was performed by Random Amplified Polymorphic DNA (RAPD) with multiple primers. Using several unusual primers and a newly designed one (DJP17), we report on the RAPD types of indigenous Campylobacter species. RAPD profiles showed different levels of discrimination between the isolates, depending on the primer: SPH1 and AG15 were informative only in part and better results were obtained with AP10, AK16 and DJP17.
format	article
author	Jošić Dragana Petković Jelena Bunčić Olivera Lepšanović Zorica Pivić Radmila Rašić Zoran Katić Vera
author_facet	Jošić Dragana Petković Jelena Bunčić Olivera Lepšanović Zorica Pivić Radmila Rašić Zoran Katić Vera
author_sort	Jošić Dragana
title	Typing of Indigenous Campylobacter spp. From Serbia by M-PCR and RAPD
title_short	Typing of Indigenous Campylobacter spp. From Serbia by M-PCR and RAPD
title_full	Typing of Indigenous Campylobacter spp. From Serbia by M-PCR and RAPD
title_fullStr	Typing of Indigenous Campylobacter spp. From Serbia by M-PCR and RAPD
title_full_unstemmed	Typing of Indigenous Campylobacter spp. From Serbia by M-PCR and RAPD
title_sort	typing of indigenous campylobacter spp. from serbia by m-pcr and rapd
publisher	Sciendo
publishDate	2016
url	https://doaj.org/article/482d81c400eb402aab5863a96961d434
work_keys_str_mv	AT josicdragana typingofindigenouscampylobactersppfromserbiabympcrandrapd AT petkovicjelena typingofindigenouscampylobactersppfromserbiabympcrandrapd AT buncicolivera typingofindigenouscampylobactersppfromserbiabympcrandrapd AT lepsanoviczorica typingofindigenouscampylobactersppfromserbiabympcrandrapd AT pivicradmila typingofindigenouscampylobactersppfromserbiabympcrandrapd AT rasiczoran typingofindigenouscampylobactersppfromserbiabympcrandrapd AT katicvera typingofindigenouscampylobactersppfromserbiabympcrandrapd
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Typing of Indigenous Campylobacter spp. From Serbia by M-PCR and RAPD

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