Detection of <named-content content-type="genus-species">Anaplasma phagocytophilum</named-content>, <italic toggle="yes">Babesia microti</italic>, <named-content content-type="genus-species">Borrelia burgdorferi</named-content>, <named-content content-type="genus-species">Borrelia miyamotoi</named-content>, and Powassan Virus in Ticks by a Multiplex Real-Time Reverse Transcription-PCR Assay

ABSTRACT Ixodes scapularis ticks are implicated in transmission of Anaplasma phagocytophilum, Borrelia burgdorferi, Borrelia miyamotoi, Babesia microti, and Powassan virus. We describe the establishment and implementation of the first multiplex real-time PCR assay with the capability to simultaneous...

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Autores principales:	Rafal Tokarz, Teresa Tagliafierro, D. Moses Cucura, Ilia Rochlin, Stephen Sameroff, W. Ian Lipkin
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2017
Materias:	Borrelia burgdorferi babesiosis ticks Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/482e3665d37840a7b85d5262882f1e23
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spelling	oai:doaj.org-article:482e3665d37840a7b85d5262882f1e232021-11-15T15:21:47ZDetection of <named-content content-type="genus-species">Anaplasma phagocytophilum</named-content>, <italic toggle="yes">Babesia microti</italic>, <named-content content-type="genus-species">Borrelia burgdorferi</named-content>, <named-content content-type="genus-species">Borrelia miyamotoi</named-content>, and Powassan Virus in Ticks by a Multiplex Real-Time Reverse Transcription-PCR Assay10.1128/mSphere.00151-172379-5042https://doaj.org/article/482e3665d37840a7b85d5262882f1e232017-04-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00151-17https://doaj.org/toc/2379-5042ABSTRACT Ixodes scapularis ticks are implicated in transmission of Anaplasma phagocytophilum, Borrelia burgdorferi, Borrelia miyamotoi, Babesia microti, and Powassan virus. We describe the establishment and implementation of the first multiplex real-time PCR assay with the capability to simultaneously detect and differentiate all five pathogens in a single reaction. The application of this assay for analysis of ticks at sites in New York and Connecticut revealed a high prevalence of B. microti in ticks from Suffolk County, NY. These findings are consistent with reports of a higher incidence of babesiosis from clinicians managing the care of patients with tick-borne diseases in this region. IMPORTANCE The understanding of pathogen prevalence is an important factor in the determination of human risks for tick-borne diseases and can help guide diagnosis and treatment. The implementation of our assay addresses a critical need in surveillance of tick-borne diseases, through generation of a comprehensive assessment of pathogen prevalence in I. scapularis. Our finding of a high frequency of ticks infected with Babesia microti in Suffolk County, NY, implicates this agent as a probable frequent cause of non-Lyme tick-borne disease in this area.Rafal TokarzTeresa TagliafierroD. Moses CucuraIlia RochlinStephen SameroffW. Ian LipkinAmerican Society for MicrobiologyarticleBorrelia burgdorferibabesiosisticksMicrobiologyQR1-502ENmSphere, Vol 2, Iss 2 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Borrelia burgdorferi babesiosis ticks Microbiology QR1-502
spellingShingle	Borrelia burgdorferi babesiosis ticks Microbiology QR1-502 Rafal Tokarz Teresa Tagliafierro D. Moses Cucura Ilia Rochlin Stephen Sameroff W. Ian Lipkin Detection of <named-content content-type="genus-species">Anaplasma phagocytophilum</named-content>, <italic toggle="yes">Babesia microti</italic>, <named-content content-type="genus-species">Borrelia burgdorferi</named-content>, <named-content content-type="genus-species">Borrelia miyamotoi</named-content>, and Powassan Virus in Ticks by a Multiplex Real-Time Reverse Transcription-PCR Assay
description	ABSTRACT Ixodes scapularis ticks are implicated in transmission of Anaplasma phagocytophilum, Borrelia burgdorferi, Borrelia miyamotoi, Babesia microti, and Powassan virus. We describe the establishment and implementation of the first multiplex real-time PCR assay with the capability to simultaneously detect and differentiate all five pathogens in a single reaction. The application of this assay for analysis of ticks at sites in New York and Connecticut revealed a high prevalence of B. microti in ticks from Suffolk County, NY. These findings are consistent with reports of a higher incidence of babesiosis from clinicians managing the care of patients with tick-borne diseases in this region. IMPORTANCE The understanding of pathogen prevalence is an important factor in the determination of human risks for tick-borne diseases and can help guide diagnosis and treatment. The implementation of our assay addresses a critical need in surveillance of tick-borne diseases, through generation of a comprehensive assessment of pathogen prevalence in I. scapularis. Our finding of a high frequency of ticks infected with Babesia microti in Suffolk County, NY, implicates this agent as a probable frequent cause of non-Lyme tick-borne disease in this area.
format	article
author	Rafal Tokarz Teresa Tagliafierro D. Moses Cucura Ilia Rochlin Stephen Sameroff W. Ian Lipkin
author_facet	Rafal Tokarz Teresa Tagliafierro D. Moses Cucura Ilia Rochlin Stephen Sameroff W. Ian Lipkin
author_sort	Rafal Tokarz
title	Detection of <named-content content-type="genus-species">Anaplasma phagocytophilum</named-content>, <italic toggle="yes">Babesia microti</italic>, <named-content content-type="genus-species">Borrelia burgdorferi</named-content>, <named-content content-type="genus-species">Borrelia miyamotoi</named-content>, and Powassan Virus in Ticks by a Multiplex Real-Time Reverse Transcription-PCR Assay
title_short	Detection of <named-content content-type="genus-species">Anaplasma phagocytophilum</named-content>, <italic toggle="yes">Babesia microti</italic>, <named-content content-type="genus-species">Borrelia burgdorferi</named-content>, <named-content content-type="genus-species">Borrelia miyamotoi</named-content>, and Powassan Virus in Ticks by a Multiplex Real-Time Reverse Transcription-PCR Assay
title_full	Detection of <named-content content-type="genus-species">Anaplasma phagocytophilum</named-content>, <italic toggle="yes">Babesia microti</italic>, <named-content content-type="genus-species">Borrelia burgdorferi</named-content>, <named-content content-type="genus-species">Borrelia miyamotoi</named-content>, and Powassan Virus in Ticks by a Multiplex Real-Time Reverse Transcription-PCR Assay
title_fullStr	Detection of <named-content content-type="genus-species">Anaplasma phagocytophilum</named-content>, <italic toggle="yes">Babesia microti</italic>, <named-content content-type="genus-species">Borrelia burgdorferi</named-content>, <named-content content-type="genus-species">Borrelia miyamotoi</named-content>, and Powassan Virus in Ticks by a Multiplex Real-Time Reverse Transcription-PCR Assay
title_full_unstemmed	Detection of <named-content content-type="genus-species">Anaplasma phagocytophilum</named-content>, <italic toggle="yes">Babesia microti</italic>, <named-content content-type="genus-species">Borrelia burgdorferi</named-content>, <named-content content-type="genus-species">Borrelia miyamotoi</named-content>, and Powassan Virus in Ticks by a Multiplex Real-Time Reverse Transcription-PCR Assay
title_sort	detection of <named-content content-type="genus-species">anaplasma phagocytophilum</named-content>, <italic toggle="yes">babesia microti</italic>, <named-content content-type="genus-species">borrelia burgdorferi</named-content>, <named-content content-type="genus-species">borrelia miyamotoi</named-content>, and powassan virus in ticks by a multiplex real-time reverse transcription-pcr assay
publisher	American Society for Microbiology
publishDate	2017
url	https://doaj.org/article/482e3665d37840a7b85d5262882f1e23
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