Study on Active Components of <i>Cuscuta chinensis</i> Promoting Neural Stem Cells Proliferation: Bioassay-Guided Fractionation

Neural stem cells (NSCs) exist in the central nervous system of adult animals and capable of self-replication. NSCs have two basic functions, namely the proliferation ability and the potential for multi-directional differentiation. In this study, based on the bioassay-guided fractionation, we aim to...

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Autores principales:	Hanze Wang, Xiaomeng Hou, Bingqi Li, Yang Yang, Qiang Li, Yinchu Si
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	neural stem cells <i>Cuscuta chinensis</i> active components network pharmacology Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/484d7b3ab55841989125d61f55a6e95b
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spelling	oai:doaj.org-article:484d7b3ab55841989125d61f55a6e95b2021-11-11T18:36:16ZStudy on Active Components of <i>Cuscuta chinensis</i> Promoting Neural Stem Cells Proliferation: Bioassay-Guided Fractionation10.3390/molecules262166341420-3049https://doaj.org/article/484d7b3ab55841989125d61f55a6e95b2021-11-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/21/6634https://doaj.org/toc/1420-3049Neural stem cells (NSCs) exist in the central nervous system of adult animals and capable of self-replication. NSCs have two basic functions, namely the proliferation ability and the potential for multi-directional differentiation. In this study, based on the bioassay-guided fractionation, we aim to screen active components in <i>Cuscuta chinensis</i> to promote the proliferation of NSCs. CCK-8 assays were used as an active detection method to track the active components. On the basis of isolating active fraction and monomer compounds, the structures of these were identified by LC-MS and (<sup>1</sup>H, <sup>13</sup>C) NMR. Moreover, active components were verified by pharmacodynamics and network pharmacology. The system solvent extraction method combined with the traditional isolation method were used to ensure that the fraction TSZE-EA-G6 of <i>Cuscuta chinensis</i> exhibited the highest activity. Seven chemical components were identified from the TSZE-EA-G6 fraction by UPLC-QE-Orbitrap-MS technology, which were 4-<i>O</i>-p-coumarinic acid, chlorogenic acid, 5-<i>O</i>-p-coumarinic acid, hyperoside, astragalin, isochlorogenic acid C, and quercetin-3-<i>O</i>-galactose-7-<i>O</i>-glucoside. Using different chromatographic techniques, five compounds were isolated in TSZE-EA-G6 and identified as kaempferol, kaempferol-3-<i>O</i>-glucoside (astragalin), quercetin-3-<i>O</i>-galactoside (hyperoside), chlorogenic acid, and sucrose. The activity study of these five compounds showed that the proliferation rate of kaempferol had the highest effects; at a certain concentration (25 μg/mL, 3.12 μg/mL), the proliferation rate could reach 87.44% and 59.59%, respectively. Furthermore, research results using network pharmacology techniques verified that kaempferol had an activity of promoting NSCs proliferation and the activity of flavonoid aglycones might be greater than that of flavonoid glycosides. In conclusion, this research shows that kaempferol is the active component in <i>Cuscuta chinensis</i> to promote the proliferation of NSCs.Hanze WangXiaomeng HouBingqi LiYang YangQiang LiYinchu SiMDPI AGarticleneural stem cells<i>Cuscuta chinensis</i>active componentsnetwork pharmacologyOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6634, p 6634 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	neural stem cells <i>Cuscuta chinensis</i> active components network pharmacology Organic chemistry QD241-441
spellingShingle	neural stem cells <i>Cuscuta chinensis</i> active components network pharmacology Organic chemistry QD241-441 Hanze Wang Xiaomeng Hou Bingqi Li Yang Yang Qiang Li Yinchu Si Study on Active Components of <i>Cuscuta chinensis</i> Promoting Neural Stem Cells Proliferation: Bioassay-Guided Fractionation
description	Neural stem cells (NSCs) exist in the central nervous system of adult animals and capable of self-replication. NSCs have two basic functions, namely the proliferation ability and the potential for multi-directional differentiation. In this study, based on the bioassay-guided fractionation, we aim to screen active components in <i>Cuscuta chinensis</i> to promote the proliferation of NSCs. CCK-8 assays were used as an active detection method to track the active components. On the basis of isolating active fraction and monomer compounds, the structures of these were identified by LC-MS and (<sup>1</sup>H, <sup>13</sup>C) NMR. Moreover, active components were verified by pharmacodynamics and network pharmacology. The system solvent extraction method combined with the traditional isolation method were used to ensure that the fraction TSZE-EA-G6 of <i>Cuscuta chinensis</i> exhibited the highest activity. Seven chemical components were identified from the TSZE-EA-G6 fraction by UPLC-QE-Orbitrap-MS technology, which were 4-<i>O</i>-p-coumarinic acid, chlorogenic acid, 5-<i>O</i>-p-coumarinic acid, hyperoside, astragalin, isochlorogenic acid C, and quercetin-3-<i>O</i>-galactose-7-<i>O</i>-glucoside. Using different chromatographic techniques, five compounds were isolated in TSZE-EA-G6 and identified as kaempferol, kaempferol-3-<i>O</i>-glucoside (astragalin), quercetin-3-<i>O</i>-galactoside (hyperoside), chlorogenic acid, and sucrose. The activity study of these five compounds showed that the proliferation rate of kaempferol had the highest effects; at a certain concentration (25 μg/mL, 3.12 μg/mL), the proliferation rate could reach 87.44% and 59.59%, respectively. Furthermore, research results using network pharmacology techniques verified that kaempferol had an activity of promoting NSCs proliferation and the activity of flavonoid aglycones might be greater than that of flavonoid glycosides. In conclusion, this research shows that kaempferol is the active component in <i>Cuscuta chinensis</i> to promote the proliferation of NSCs.
format	article
author	Hanze Wang Xiaomeng Hou Bingqi Li Yang Yang Qiang Li Yinchu Si
author_facet	Hanze Wang Xiaomeng Hou Bingqi Li Yang Yang Qiang Li Yinchu Si
author_sort	Hanze Wang
title	Study on Active Components of <i>Cuscuta chinensis</i> Promoting Neural Stem Cells Proliferation: Bioassay-Guided Fractionation
title_short	Study on Active Components of <i>Cuscuta chinensis</i> Promoting Neural Stem Cells Proliferation: Bioassay-Guided Fractionation
title_full	Study on Active Components of <i>Cuscuta chinensis</i> Promoting Neural Stem Cells Proliferation: Bioassay-Guided Fractionation
title_fullStr	Study on Active Components of <i>Cuscuta chinensis</i> Promoting Neural Stem Cells Proliferation: Bioassay-Guided Fractionation
title_full_unstemmed	Study on Active Components of <i>Cuscuta chinensis</i> Promoting Neural Stem Cells Proliferation: Bioassay-Guided Fractionation
title_sort	study on active components of <i>cuscuta chinensis</i> promoting neural stem cells proliferation: bioassay-guided fractionation
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/484d7b3ab55841989125d61f55a6e95b
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Study on Active Components of <i>Cuscuta chinensis</i> Promoting Neural Stem Cells Proliferation: Bioassay-Guided Fractionation

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