Assessing fatty acid-induced lipotoxicity and its therapeutic potential in glioblastoma using stimulated Raman microscopy

Abstract Glioblastoma multiforme (GBM) is the most aggressive primary brain tumor. The effectiveness of traditional therapies for GBM is limited and therefore new therapies are highly desired. Previous studies show that lipid metabolism reprogramming may be a potential therapeutic target in GBM. Thi...

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Autores principales:	Yuhao Yuan, Niraj Shah, Mohammad I. Almohaisin, Soumit Saha, Fake Lu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/484e27ba0eac430f89dbe1d5dc0b5ebe
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spelling	oai:doaj.org-article:484e27ba0eac430f89dbe1d5dc0b5ebe2021-12-02T18:18:06ZAssessing fatty acid-induced lipotoxicity and its therapeutic potential in glioblastoma using stimulated Raman microscopy10.1038/s41598-021-86789-92045-2322https://doaj.org/article/484e27ba0eac430f89dbe1d5dc0b5ebe2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86789-9https://doaj.org/toc/2045-2322Abstract Glioblastoma multiforme (GBM) is the most aggressive primary brain tumor. The effectiveness of traditional therapies for GBM is limited and therefore new therapies are highly desired. Previous studies show that lipid metabolism reprogramming may be a potential therapeutic target in GBM. This study aims to evaluate the therapeutic potential of free fatty acid-induced lipotoxicity for the suppression of glioma growth. U87 glioma cells are treated with three fatty acids (FAs): palmitic acid (PA), oleic acid (OA), and eicosapentaenoic acid (EPA). Uptake of the FAs and formation of lipid droplets (LDs) are imaged and quantified using a lab-built stimulated Raman scattering (SRS) microscope. Our results show that a supply of 200 µM PA, OA, and EPA leads to efficient LDs accumulation in glioma cells. We find that inhibition of triglycerides (TAGs) synthesis depletes LDs and enhances lipotoxicity, which is evidenced by the reduced cell proliferation rates. In particular, our results suggest that EPA treatment combined with depletion of LDs significantly reduces the survival rate of glioma cells by more than 50%, indicating the therapeutic potential of this approach. Future work will focus on understanding the metabolic mechanism of EPA-induced lipotoxicity to further enhance its anticancer effects.Yuhao YuanNiraj ShahMohammad I. AlmohaisinSoumit SahaFake LuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yuhao Yuan Niraj Shah Mohammad I. Almohaisin Soumit Saha Fake Lu Assessing fatty acid-induced lipotoxicity and its therapeutic potential in glioblastoma using stimulated Raman microscopy
description	Abstract Glioblastoma multiforme (GBM) is the most aggressive primary brain tumor. The effectiveness of traditional therapies for GBM is limited and therefore new therapies are highly desired. Previous studies show that lipid metabolism reprogramming may be a potential therapeutic target in GBM. This study aims to evaluate the therapeutic potential of free fatty acid-induced lipotoxicity for the suppression of glioma growth. U87 glioma cells are treated with three fatty acids (FAs): palmitic acid (PA), oleic acid (OA), and eicosapentaenoic acid (EPA). Uptake of the FAs and formation of lipid droplets (LDs) are imaged and quantified using a lab-built stimulated Raman scattering (SRS) microscope. Our results show that a supply of 200 µM PA, OA, and EPA leads to efficient LDs accumulation in glioma cells. We find that inhibition of triglycerides (TAGs) synthesis depletes LDs and enhances lipotoxicity, which is evidenced by the reduced cell proliferation rates. In particular, our results suggest that EPA treatment combined with depletion of LDs significantly reduces the survival rate of glioma cells by more than 50%, indicating the therapeutic potential of this approach. Future work will focus on understanding the metabolic mechanism of EPA-induced lipotoxicity to further enhance its anticancer effects.
format	article
author	Yuhao Yuan Niraj Shah Mohammad I. Almohaisin Soumit Saha Fake Lu
author_facet	Yuhao Yuan Niraj Shah Mohammad I. Almohaisin Soumit Saha Fake Lu
author_sort	Yuhao Yuan
title	Assessing fatty acid-induced lipotoxicity and its therapeutic potential in glioblastoma using stimulated Raman microscopy
title_short	Assessing fatty acid-induced lipotoxicity and its therapeutic potential in glioblastoma using stimulated Raman microscopy
title_full	Assessing fatty acid-induced lipotoxicity and its therapeutic potential in glioblastoma using stimulated Raman microscopy
title_fullStr	Assessing fatty acid-induced lipotoxicity and its therapeutic potential in glioblastoma using stimulated Raman microscopy
title_full_unstemmed	Assessing fatty acid-induced lipotoxicity and its therapeutic potential in glioblastoma using stimulated Raman microscopy
title_sort	assessing fatty acid-induced lipotoxicity and its therapeutic potential in glioblastoma using stimulated raman microscopy
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/484e27ba0eac430f89dbe1d5dc0b5ebe
work_keys_str_mv	AT yuhaoyuan assessingfattyacidinducedlipotoxicityanditstherapeuticpotentialinglioblastomausingstimulatedramanmicroscopy AT nirajshah assessingfattyacidinducedlipotoxicityanditstherapeuticpotentialinglioblastomausingstimulatedramanmicroscopy AT mohammadialmohaisin assessingfattyacidinducedlipotoxicityanditstherapeuticpotentialinglioblastomausingstimulatedramanmicroscopy AT soumitsaha assessingfattyacidinducedlipotoxicityanditstherapeuticpotentialinglioblastomausingstimulatedramanmicroscopy AT fakelu assessingfattyacidinducedlipotoxicityanditstherapeuticpotentialinglioblastomausingstimulatedramanmicroscopy
_version_	1718378271009144832

Assessing fatty acid-induced lipotoxicity and its therapeutic potential in glioblastoma using stimulated Raman microscopy

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