Enhanced reduction in cell viability by hyperthermia induced by magnetic nanoparticles

H&eacute;ctor L Rodr&iacute;guez-Luccioni, Magda Latorre-Esteves, Janet M&eacute;ndez-Vega, Orlando Soto, Ana R Rodr&iacute;guez, Carlos Rinaldi, Madeline Torres-LugoDepartment of Chemical Engineering, University of Puerto Rico, Mayag&uuml;ez 00681, Puerto Ric...

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Autores principales:	H&eacute;ctor L Rodr&iacute;guez-Luccioni, Magda Latorre-Esteves, Janet M&eacute;ndez-Vega, et al
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/4853ef7deeef4f4f927a16bc6e2c514c
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spelling	oai:doaj.org-article:4853ef7deeef4f4f927a16bc6e2c514c2021-12-02T06:29:10ZEnhanced reduction in cell viability by hyperthermia induced by magnetic nanoparticles1176-91141178-2013https://doaj.org/article/4853ef7deeef4f4f927a16bc6e2c514c2011-02-01T00:00:00Zhttp://www.dovepress.com/enhanced-reduction-in-cell-viability-by-hyperthermia-induced-by-magnet-a6355https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013H&eacute;ctor L Rodr&iacute;guez-Luccioni, Magda Latorre-Esteves, Janet M&eacute;ndez-Vega, Orlando Soto, Ana R Rodr&iacute;guez, Carlos Rinaldi, Madeline Torres-LugoDepartment of Chemical Engineering, University of Puerto Rico, Mayag&uuml;ez 00681, Puerto RicoAbstract: Colloidal suspensions of iron oxide magnetic nanoparticles are known to dissipate energy when exposed to an oscillating magnetic field. Such energy dissipation can be employed to locally raise temperature inside a tumor between 41&deg;C and 45&deg;C (hyperthermia) to promote cell death, a treatment known as magnetic fluid hyperthermia (MFH). This work seeks to quantify differences between MFH and hot-water hyperthermia (HWH) in terms of reduction in cell viability using two cancer cell culture models, Caco-2 (human epithelial colorectal adenocarcinoma) and MCF-7 (human breast cancer). Magnetite nanoparticles were synthesized via the co-precipitation method and functionalized with adsorbed carboxymethyl dextran. Cytotoxicity studies indicated that in the absence of an oscillating magnetic field, cell viability was not affected at concentrations of up to 0.6 mg iron oxide/mL. MFH resulted in a significant decrease in cell viability when exposed to a magnetic field for 120 minutes and allowed to rest for 48 hours, compared with similar field applications, but with shorter resting time. The results presented here suggest that MFH most likely induces apoptosis in both cell types. When compared with HWH, MFH produced a significant reduction in cell viability, and these effects appear to be cell-type related.Keywords: magnetic fluid hyperthermia, carboxymethyl dextran magnetite, cell death, apoptosis H&eacute;ctor L Rodr&iacute;guez-LuccioniMagda Latorre-EstevesJanet M&eacute;ndez-Vegaet alDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 373-380 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 H&eacute;ctor L Rodr&iacute;guez-Luccioni Magda Latorre-Esteves Janet M&eacute;ndez-Vega et al Enhanced reduction in cell viability by hyperthermia induced by magnetic nanoparticles
description	H&eacute;ctor L Rodr&iacute;guez-Luccioni, Magda Latorre-Esteves, Janet M&eacute;ndez-Vega, Orlando Soto, Ana R Rodr&iacute;guez, Carlos Rinaldi, Madeline Torres-LugoDepartment of Chemical Engineering, University of Puerto Rico, Mayag&uuml;ez 00681, Puerto RicoAbstract: Colloidal suspensions of iron oxide magnetic nanoparticles are known to dissipate energy when exposed to an oscillating magnetic field. Such energy dissipation can be employed to locally raise temperature inside a tumor between 41&deg;C and 45&deg;C (hyperthermia) to promote cell death, a treatment known as magnetic fluid hyperthermia (MFH). This work seeks to quantify differences between MFH and hot-water hyperthermia (HWH) in terms of reduction in cell viability using two cancer cell culture models, Caco-2 (human epithelial colorectal adenocarcinoma) and MCF-7 (human breast cancer). Magnetite nanoparticles were synthesized via the co-precipitation method and functionalized with adsorbed carboxymethyl dextran. Cytotoxicity studies indicated that in the absence of an oscillating magnetic field, cell viability was not affected at concentrations of up to 0.6 mg iron oxide/mL. MFH resulted in a significant decrease in cell viability when exposed to a magnetic field for 120 minutes and allowed to rest for 48 hours, compared with similar field applications, but with shorter resting time. The results presented here suggest that MFH most likely induces apoptosis in both cell types. When compared with HWH, MFH produced a significant reduction in cell viability, and these effects appear to be cell-type related.Keywords: magnetic fluid hyperthermia, carboxymethyl dextran magnetite, cell death, apoptosis
format	article
author	H&eacute;ctor L Rodr&iacute;guez-Luccioni Magda Latorre-Esteves Janet M&eacute;ndez-Vega et al
author_facet	H&eacute;ctor L Rodr&iacute;guez-Luccioni Magda Latorre-Esteves Janet M&eacute;ndez-Vega et al
author_sort	H&eacute;ctor L Rodr&iacute;guez-Luccioni
title	Enhanced reduction in cell viability by hyperthermia induced by magnetic nanoparticles
title_short	Enhanced reduction in cell viability by hyperthermia induced by magnetic nanoparticles
title_full	Enhanced reduction in cell viability by hyperthermia induced by magnetic nanoparticles
title_fullStr	Enhanced reduction in cell viability by hyperthermia induced by magnetic nanoparticles
title_full_unstemmed	Enhanced reduction in cell viability by hyperthermia induced by magnetic nanoparticles
title_sort	enhanced reduction in cell viability by hyperthermia induced by magnetic nanoparticles
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/4853ef7deeef4f4f927a16bc6e2c514c
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Enhanced reduction in cell viability by hyperthermia induced by magnetic nanoparticles

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