Phage lysin that specifically eliminates Clostridium botulinum Group I cells

Abstract Clostridium botulinum poses a serious threat to food safety and public health by producing potent neurotoxin during its vegetative growth and causing life-threatening neuroparalysis, botulism. While high temperature can be utilized to eliminate C. botulinum spores and the neurotoxin, non-th...

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Autores principales:	Zhen Zhang, Meeri Lahti, François P. Douillard, Hannu Korkeala, Miia Lindström
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/48609049a8094abeb1eeb428ae051a57
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spelling	oai:doaj.org-article:48609049a8094abeb1eeb428ae051a572021-12-02T16:18:04ZPhage lysin that specifically eliminates Clostridium botulinum Group I cells10.1038/s41598-020-78622-62045-2322https://doaj.org/article/48609049a8094abeb1eeb428ae051a572020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78622-6https://doaj.org/toc/2045-2322Abstract Clostridium botulinum poses a serious threat to food safety and public health by producing potent neurotoxin during its vegetative growth and causing life-threatening neuroparalysis, botulism. While high temperature can be utilized to eliminate C. botulinum spores and the neurotoxin, non-thermal elimination of newly germinated C. botulinum cells before onset of toxin production could provide an alternative or additional factor controlling the risk of botulism in some applications. Here we introduce a putative phage lysin that specifically lyses vegetative C. botulinum Group I cells. This lysin, called CBO1751, efficiently kills cells of C. botulinum Group I strains at the concentration of 5 µM, but shows little or no lytic activity against C. botulinum Group II or III or other Firmicutes strains. CBO1751 is active at pH from 6.5 to 10.5. The lytic activity of CBO1751 is tolerant to NaCl (200 mM), but highly susceptible to divalent cations Ca2+ and Mg2+ (50 mM). CBO1751 readily and effectively eliminates C. botulinum during spore germination, an early stage preceding vegetative growth and neurotoxin production. This is the first report of an antimicrobial lysin against C. botulinum, presenting high potential for developing a novel antibotulinal agent for non-thermal applications in food and agricultural industries.Zhen ZhangMeeri LahtiFrançois P. DouillardHannu KorkealaMiia LindströmNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-10 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zhen Zhang Meeri Lahti François P. Douillard Hannu Korkeala Miia Lindström Phage lysin that specifically eliminates Clostridium botulinum Group I cells
description	Abstract Clostridium botulinum poses a serious threat to food safety and public health by producing potent neurotoxin during its vegetative growth and causing life-threatening neuroparalysis, botulism. While high temperature can be utilized to eliminate C. botulinum spores and the neurotoxin, non-thermal elimination of newly germinated C. botulinum cells before onset of toxin production could provide an alternative or additional factor controlling the risk of botulism in some applications. Here we introduce a putative phage lysin that specifically lyses vegetative C. botulinum Group I cells. This lysin, called CBO1751, efficiently kills cells of C. botulinum Group I strains at the concentration of 5 µM, but shows little or no lytic activity against C. botulinum Group II or III or other Firmicutes strains. CBO1751 is active at pH from 6.5 to 10.5. The lytic activity of CBO1751 is tolerant to NaCl (200 mM), but highly susceptible to divalent cations Ca2+ and Mg2+ (50 mM). CBO1751 readily and effectively eliminates C. botulinum during spore germination, an early stage preceding vegetative growth and neurotoxin production. This is the first report of an antimicrobial lysin against C. botulinum, presenting high potential for developing a novel antibotulinal agent for non-thermal applications in food and agricultural industries.
format	article
author	Zhen Zhang Meeri Lahti François P. Douillard Hannu Korkeala Miia Lindström
author_facet	Zhen Zhang Meeri Lahti François P. Douillard Hannu Korkeala Miia Lindström
author_sort	Zhen Zhang
title	Phage lysin that specifically eliminates Clostridium botulinum Group I cells
title_short	Phage lysin that specifically eliminates Clostridium botulinum Group I cells
title_full	Phage lysin that specifically eliminates Clostridium botulinum Group I cells
title_fullStr	Phage lysin that specifically eliminates Clostridium botulinum Group I cells
title_full_unstemmed	Phage lysin that specifically eliminates Clostridium botulinum Group I cells
title_sort	phage lysin that specifically eliminates clostridium botulinum group i cells
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/48609049a8094abeb1eeb428ae051a57
work_keys_str_mv	AT zhenzhang phagelysinthatspecificallyeliminatesclostridiumbotulinumgroupicells AT meerilahti phagelysinthatspecificallyeliminatesclostridiumbotulinumgroupicells AT francoispdouillard phagelysinthatspecificallyeliminatesclostridiumbotulinumgroupicells AT hannukorkeala phagelysinthatspecificallyeliminatesclostridiumbotulinumgroupicells AT miialindstrom phagelysinthatspecificallyeliminatesclostridiumbotulinumgroupicells
_version_	1718384165550817280

Phage lysin that specifically eliminates Clostridium botulinum Group I cells

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