Nuclear Morphometry as an Adjunct to Cytomorphology in the Diagnosis of Thyroid Lesions

Introduction: Fine Needle Aspiration Cytology (FNAC) is a reliable and reproducible diagnostic technique for thyroid lesions. Recently, it has been suggested that evaluation of nuclear features may enhance the diagnostic utility of FNAC. However, the evaluation of nuclear morphometry is not well...

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Autores principales:	Sabina Khan, Sayika Hameed, Mohd Jaseem Hasan, Musharraf Husain, Arun Sharma
Formato:	article
Lenguaje:	EN
Publicado:	JCDR Research and Publications Private Limited 2021
Materias:	aspiration cytology histopathological photomicrography thyroid aspirates Medicine R
Acceso en línea:	https://doaj.org/article/4863537c5b57420798f5de6eaed9bdcf
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spelling	oai:doaj.org-article:4863537c5b57420798f5de6eaed9bdcf2021-11-07T10:07:10ZNuclear Morphometry as an Adjunct to Cytomorphology in the Diagnosis of Thyroid Lesions10.7860/JCDR/2021/49980.153312249-782X0973-709Xhttps://doaj.org/article/4863537c5b57420798f5de6eaed9bdcf2021-09-01T00:00:00Zhttps://www.jcdr.net/articles/PDF/15331/49980_CE[Ra1]_F(SHU)_PF1(AKA_RK_KM)PN(KM).pdfhttps://doaj.org/toc/2249-782Xhttps://doaj.org/toc/0973-709XIntroduction: Fine Needle Aspiration Cytology (FNAC) is a reliable and reproducible diagnostic technique for thyroid lesions. Recently, it has been suggested that evaluation of nuclear features may enhance the diagnostic utility of FNAC. However, the evaluation of nuclear morphometry is not well established in thyroid cytology. Aim: To evaluate the role of nuclear morphometry in cytological evaluation of thyroid lesions. Materials and Methods: This was a cross-sectional study conducted over a period from March 2019-February 2020 at Hamdard Institute of Medical Sciences and Research, New Delhi, India. Morphometry was done on 40 cases of thyroid aspirates which had histopathological concordance. Computerised nuclear morphometry was done by using photographs captured under Motic photomicrography system. Six parameters were measured- nuclear area, nuclear perimeter, minimal nuclear diameter, maximal nuclear diameter, nuclear compactness and LS ratio (Largest to Smallest dimension ratio). Data were entered in spreadsheet and then analysed using Statistical Package for Social Sciences (SPSS) version 20.0. Results: Out of total 40 thyroid aspirates studied, included non neoplastic (19 cases), benign (12 cases) and malignant lesions (9 cases). All nuclear morphometry parameters comprising of nuclear area, nuclear perimeter, minimal nuclear diameter, maximal nuclear diameter showed an increasing trend from non neoplastic to benign to malignant with a statistically significant difference between benign and malignant groups (p-values <0.05) except for LS ratio and nuclear compactness. Conclusion: Nuclear morphometry can aid in cytological diagnosis of thyroid lesions. If used judiciously, quantitative estimation of cytological nuclear features can be helpful in assessing thyroid lesions preoperatively thus complementing its cytomorphological features.Sabina KhanSayika HameedMohd Jaseem HasanMusharraf HusainArun SharmaJCDR Research and Publications Private Limitedarticleaspiration cytologyhistopathologicalphotomicrographythyroid aspiratesMedicineRENJournal of Clinical and Diagnostic Research, Vol 15, Iss 9, Pp EC01-EC04 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	aspiration cytology histopathological photomicrography thyroid aspirates Medicine R
spellingShingle	aspiration cytology histopathological photomicrography thyroid aspirates Medicine R Sabina Khan Sayika Hameed Mohd Jaseem Hasan Musharraf Husain Arun Sharma Nuclear Morphometry as an Adjunct to Cytomorphology in the Diagnosis of Thyroid Lesions
description	Introduction: Fine Needle Aspiration Cytology (FNAC) is a reliable and reproducible diagnostic technique for thyroid lesions. Recently, it has been suggested that evaluation of nuclear features may enhance the diagnostic utility of FNAC. However, the evaluation of nuclear morphometry is not well established in thyroid cytology. Aim: To evaluate the role of nuclear morphometry in cytological evaluation of thyroid lesions. Materials and Methods: This was a cross-sectional study conducted over a period from March 2019-February 2020 at Hamdard Institute of Medical Sciences and Research, New Delhi, India. Morphometry was done on 40 cases of thyroid aspirates which had histopathological concordance. Computerised nuclear morphometry was done by using photographs captured under Motic photomicrography system. Six parameters were measured- nuclear area, nuclear perimeter, minimal nuclear diameter, maximal nuclear diameter, nuclear compactness and LS ratio (Largest to Smallest dimension ratio). Data were entered in spreadsheet and then analysed using Statistical Package for Social Sciences (SPSS) version 20.0. Results: Out of total 40 thyroid aspirates studied, included non neoplastic (19 cases), benign (12 cases) and malignant lesions (9 cases). All nuclear morphometry parameters comprising of nuclear area, nuclear perimeter, minimal nuclear diameter, maximal nuclear diameter showed an increasing trend from non neoplastic to benign to malignant with a statistically significant difference between benign and malignant groups (p-values <0.05) except for LS ratio and nuclear compactness. Conclusion: Nuclear morphometry can aid in cytological diagnosis of thyroid lesions. If used judiciously, quantitative estimation of cytological nuclear features can be helpful in assessing thyroid lesions preoperatively thus complementing its cytomorphological features.
format	article
author	Sabina Khan Sayika Hameed Mohd Jaseem Hasan Musharraf Husain Arun Sharma
author_facet	Sabina Khan Sayika Hameed Mohd Jaseem Hasan Musharraf Husain Arun Sharma
author_sort	Sabina Khan
title	Nuclear Morphometry as an Adjunct to Cytomorphology in the Diagnosis of Thyroid Lesions
title_short	Nuclear Morphometry as an Adjunct to Cytomorphology in the Diagnosis of Thyroid Lesions
title_full	Nuclear Morphometry as an Adjunct to Cytomorphology in the Diagnosis of Thyroid Lesions
title_fullStr	Nuclear Morphometry as an Adjunct to Cytomorphology in the Diagnosis of Thyroid Lesions
title_full_unstemmed	Nuclear Morphometry as an Adjunct to Cytomorphology in the Diagnosis of Thyroid Lesions
title_sort	nuclear morphometry as an adjunct to cytomorphology in the diagnosis of thyroid lesions
publisher	JCDR Research and Publications Private Limited
publishDate	2021
url	https://doaj.org/article/4863537c5b57420798f5de6eaed9bdcf
work_keys_str_mv	AT sabinakhan nuclearmorphometryasanadjuncttocytomorphologyinthediagnosisofthyroidlesions AT sayikahameed nuclearmorphometryasanadjuncttocytomorphologyinthediagnosisofthyroidlesions AT mohdjaseemhasan nuclearmorphometryasanadjuncttocytomorphologyinthediagnosisofthyroidlesions AT musharrafhusain nuclearmorphometryasanadjuncttocytomorphologyinthediagnosisofthyroidlesions AT arunsharma nuclearmorphometryasanadjuncttocytomorphologyinthediagnosisofthyroidlesions
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Nuclear Morphometry as an Adjunct to Cytomorphology in the Diagnosis of Thyroid Lesions

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