Surface decoration by Spirulina polysaccharide enhances the cellular uptake and anticancer efficacy of selenium nanoparticles

Fang Yang1*, Quanming Tang1,2*, Xueyun Zhong3, Yan Bai1, Tianfeng Chen1, Yibo Zhang1, Yinghua Li1, Wenjie Zheng11Department of Chemistry, Jinan University, Guangzhou, China; 2South China Seas Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, China; 3Department of Pathology, Jinan Univ...

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Autores principales:	Li Y, Zhang Y, Chen T, Bai Y, Zhong X, Tang Q, Yang F, Zheng W
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/48699ce0f908490696cc859fe3ec9edc
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spelling	oai:doaj.org-article:48699ce0f908490696cc859fe3ec9edc2021-12-02T02:04:24ZSurface decoration by Spirulina polysaccharide enhances the cellular uptake and anticancer efficacy of selenium nanoparticles1176-91141178-2013https://doaj.org/article/48699ce0f908490696cc859fe3ec9edc2012-02-01T00:00:00Zhttp://www.dovepress.com/surface-decoration-by-spirulina-polysaccharide-enhances-the-cellular-u-a9294https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Fang Yang1, Quanming Tang1,2, Xueyun Zhong3, Yan Bai1, Tianfeng Chen1, Yibo Zhang1, Yinghua Li1, Wenjie Zheng11Department of Chemistry, Jinan University, Guangzhou, China; 2South China Seas Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, China; 3Department of Pathology, Jinan University, Guangzhou, China*These authors contributed equally to this workAbstract: A simple and solution-phase method for functionalization of selenium nanoparticles (SeNPs) with Spirulina polysaccharides (SPS) has been developed in the present study. The cellular uptake and anticancer activity of SPS-SeNPs were also evaluated. Monodisperse and homogeneous spherical SPS-SeNPs with diameters ranging from 20 nm to 50 nm were achieved under optimized conditions, which were stable in the solution phase for at least 3 months. SPS surface decoration significantly enhanced the cellular uptake and cytotoxicity of SeNPs toward several human cancer cell lines. A375 human melanoma cells were found extremely susceptible to SPS-SeNPs with half maximal (50%) inhibitory concentration value of 7.94 &micro;M. Investigation of the underlying mechanisms revealed that SPS-SeNPs inhibited cancer cell growth through induction of apoptosis, as evidenced by an increase in sub-G1 cell population, deoxyribonucleic acid fragmentation, chromatin condensation, and phosphatidylserine translocation. Results suggest that the strategy to use SPS as a surface decorator could be an effective way to enhance the cellular uptake and anticancer efficacy of nanomaterials. SPS-SeNPs may be a potential candidate for further evaluation as a chemopreventive and chemotherapeutic agent against human cancers.Keywords: selenium nanoparticles, Spirulina polysaccharide, cellular uptake, anticancer, apoptosisLi YZhang YChen TBai YZhong XTang QYang FZheng WDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 835-844 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Li Y Zhang Y Chen T Bai Y Zhong X Tang Q Yang F Zheng W Surface decoration by Spirulina polysaccharide enhances the cellular uptake and anticancer efficacy of selenium nanoparticles
description	Fang Yang1, Quanming Tang1,2, Xueyun Zhong3, Yan Bai1, Tianfeng Chen1, Yibo Zhang1, Yinghua Li1, Wenjie Zheng11Department of Chemistry, Jinan University, Guangzhou, China; 2South China Seas Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, China; 3Department of Pathology, Jinan University, Guangzhou, China*These authors contributed equally to this workAbstract: A simple and solution-phase method for functionalization of selenium nanoparticles (SeNPs) with Spirulina polysaccharides (SPS) has been developed in the present study. The cellular uptake and anticancer activity of SPS-SeNPs were also evaluated. Monodisperse and homogeneous spherical SPS-SeNPs with diameters ranging from 20 nm to 50 nm were achieved under optimized conditions, which were stable in the solution phase for at least 3 months. SPS surface decoration significantly enhanced the cellular uptake and cytotoxicity of SeNPs toward several human cancer cell lines. A375 human melanoma cells were found extremely susceptible to SPS-SeNPs with half maximal (50%) inhibitory concentration value of 7.94 &micro;M. Investigation of the underlying mechanisms revealed that SPS-SeNPs inhibited cancer cell growth through induction of apoptosis, as evidenced by an increase in sub-G1 cell population, deoxyribonucleic acid fragmentation, chromatin condensation, and phosphatidylserine translocation. Results suggest that the strategy to use SPS as a surface decorator could be an effective way to enhance the cellular uptake and anticancer efficacy of nanomaterials. SPS-SeNPs may be a potential candidate for further evaluation as a chemopreventive and chemotherapeutic agent against human cancers.Keywords: selenium nanoparticles, Spirulina polysaccharide, cellular uptake, anticancer, apoptosis
format	article
author	Li Y Zhang Y Chen T Bai Y Zhong X Tang Q Yang F Zheng W
author_facet	Li Y Zhang Y Chen T Bai Y Zhong X Tang Q Yang F Zheng W
author_sort	Li Y
title	Surface decoration by Spirulina polysaccharide enhances the cellular uptake and anticancer efficacy of selenium nanoparticles
title_short	Surface decoration by Spirulina polysaccharide enhances the cellular uptake and anticancer efficacy of selenium nanoparticles
title_full	Surface decoration by Spirulina polysaccharide enhances the cellular uptake and anticancer efficacy of selenium nanoparticles
title_fullStr	Surface decoration by Spirulina polysaccharide enhances the cellular uptake and anticancer efficacy of selenium nanoparticles
title_full_unstemmed	Surface decoration by Spirulina polysaccharide enhances the cellular uptake and anticancer efficacy of selenium nanoparticles
title_sort	surface decoration by spirulina polysaccharide enhances the cellular uptake and anticancer efficacy of selenium nanoparticles
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/48699ce0f908490696cc859fe3ec9edc
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Surface decoration by Spirulina polysaccharide enhances the cellular uptake and anticancer efficacy of selenium nanoparticles

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