Biodegradation of chlorpyrifos using isolates from contaminated agricultural soil, its kinetic studies

Abstract Extensive pesticides use is negatively disturbing the environment and humans. Pesticide bioremediation with eco-friendly techniques bears prime importance. This study evaluates the bioremediation of chlorpyrifos in soil using indigenous Bacillus cereus Ct3, isolated from cotton growing soil...

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Autores principales:	Muhammad Farhan, Maqsood Ahmad, Amina Kanwal, Zahid Ali Butt, Qaiser Farid Khan, Syed Ali Raza, Haleema Qayyum, Abdul Wahid
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/486cea073b814fe894579c4c69e59665
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spelling	oai:doaj.org-article:486cea073b814fe894579c4c69e596652021-12-02T17:15:24ZBiodegradation of chlorpyrifos using isolates from contaminated agricultural soil, its kinetic studies10.1038/s41598-021-88264-x2045-2322https://doaj.org/article/486cea073b814fe894579c4c69e596652021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88264-xhttps://doaj.org/toc/2045-2322Abstract Extensive pesticides use is negatively disturbing the environment and humans. Pesticide bioremediation with eco-friendly techniques bears prime importance. This study evaluates the bioremediation of chlorpyrifos in soil using indigenous Bacillus cereus Ct3, isolated from cotton growing soils. Strains were identified through ribotyping (16s rRNA) by Macrogen (Macrogen Inc. Geumchen-gu, South Korea). Bacillus cereus Ct3 was resistant up to 125 mg L−1 of chlorpyrifos and successfully degraded 88% of chlorpyfifos in 8 days at pH 8. Bacillus cereus Ct3 tolerated about 30–40 °C of temperature, this is a good sign for in situ bioremediation. Green compost, farmyard manure and rice husk were tested, where ANOVA (P < 0.05) and Plackett–Burman design, results indicated that the farm yard manure has significant impact on degradation. It reduced the lag phase and brought maximum degradation up to 88%. Inoculum size is a statistically significant (P < 0.05) factor and below 106 (CFU g−1) show lag phase of 4–6 days. Michaelis–Menten model results were as follows; R2 = 0.9919, Vmax = 18.8, Ks = 121.4 and Vmax/Ks = 0.1546. GC–MS study revealed that chlorpyrifos first converted into diethylthiophosphoric acid and 3,5,6-trichloro-2-pyridinol (TCP). Later, TCP ring was broken and it was completely mineralized without any toxic byproduct. Plackett–Burman design was employed to investigate the effect of five factors. The correlation coefficient (R2) between experimental and predicted value is 0.94. Central composite design (CBD) was employed with design matrix of thirty one predicted and experimental values of chlorpyrifos degradation, having “lack of fit P value” of “0.00”. The regression coefficient obtained was R2 = 0.93 which indicate that the experimental vales and the predicted values are closely fitted. The most significant factors highlighted in CBD/ANOVA and surface response plots were chlorpyrifor concentration and inoculum size. Bacillus cereus Ct3 effectively degraded chlorpyrifos and can successfully be used for bioremediation of chlorpyrifos contaminated soils.Muhammad FarhanMaqsood AhmadAmina KanwalZahid Ali ButtQaiser Farid KhanSyed Ali RazaHaleema QayyumAbdul WahidNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Muhammad Farhan Maqsood Ahmad Amina Kanwal Zahid Ali Butt Qaiser Farid Khan Syed Ali Raza Haleema Qayyum Abdul Wahid Biodegradation of chlorpyrifos using isolates from contaminated agricultural soil, its kinetic studies
description	Abstract Extensive pesticides use is negatively disturbing the environment and humans. Pesticide bioremediation with eco-friendly techniques bears prime importance. This study evaluates the bioremediation of chlorpyrifos in soil using indigenous Bacillus cereus Ct3, isolated from cotton growing soils. Strains were identified through ribotyping (16s rRNA) by Macrogen (Macrogen Inc. Geumchen-gu, South Korea). Bacillus cereus Ct3 was resistant up to 125 mg L−1 of chlorpyrifos and successfully degraded 88% of chlorpyfifos in 8 days at pH 8. Bacillus cereus Ct3 tolerated about 30–40 °C of temperature, this is a good sign for in situ bioremediation. Green compost, farmyard manure and rice husk were tested, where ANOVA (P < 0.05) and Plackett–Burman design, results indicated that the farm yard manure has significant impact on degradation. It reduced the lag phase and brought maximum degradation up to 88%. Inoculum size is a statistically significant (P < 0.05) factor and below 106 (CFU g−1) show lag phase of 4–6 days. Michaelis–Menten model results were as follows; R2 = 0.9919, Vmax = 18.8, Ks = 121.4 and Vmax/Ks = 0.1546. GC–MS study revealed that chlorpyrifos first converted into diethylthiophosphoric acid and 3,5,6-trichloro-2-pyridinol (TCP). Later, TCP ring was broken and it was completely mineralized without any toxic byproduct. Plackett–Burman design was employed to investigate the effect of five factors. The correlation coefficient (R2) between experimental and predicted value is 0.94. Central composite design (CBD) was employed with design matrix of thirty one predicted and experimental values of chlorpyrifos degradation, having “lack of fit P value” of “0.00”. The regression coefficient obtained was R2 = 0.93 which indicate that the experimental vales and the predicted values are closely fitted. The most significant factors highlighted in CBD/ANOVA and surface response plots were chlorpyrifor concentration and inoculum size. Bacillus cereus Ct3 effectively degraded chlorpyrifos and can successfully be used for bioremediation of chlorpyrifos contaminated soils.
format	article
author	Muhammad Farhan Maqsood Ahmad Amina Kanwal Zahid Ali Butt Qaiser Farid Khan Syed Ali Raza Haleema Qayyum Abdul Wahid
author_facet	Muhammad Farhan Maqsood Ahmad Amina Kanwal Zahid Ali Butt Qaiser Farid Khan Syed Ali Raza Haleema Qayyum Abdul Wahid
author_sort	Muhammad Farhan
title	Biodegradation of chlorpyrifos using isolates from contaminated agricultural soil, its kinetic studies
title_short	Biodegradation of chlorpyrifos using isolates from contaminated agricultural soil, its kinetic studies
title_full	Biodegradation of chlorpyrifos using isolates from contaminated agricultural soil, its kinetic studies
title_fullStr	Biodegradation of chlorpyrifos using isolates from contaminated agricultural soil, its kinetic studies
title_full_unstemmed	Biodegradation of chlorpyrifos using isolates from contaminated agricultural soil, its kinetic studies
title_sort	biodegradation of chlorpyrifos using isolates from contaminated agricultural soil, its kinetic studies
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/486cea073b814fe894579c4c69e59665
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Biodegradation of chlorpyrifos using isolates from contaminated agricultural soil, its kinetic studies

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