Expression of STOML2 promotes proliferation and glycolysis of multiple myeloma cells via upregulating PAI-1

Abstract Background This study aimed to investigate the effects of STOML2 and the relationship between STOML2 and PAI-1 in the development of multiple myeloma (MM). Methods Cell proliferation was tested using CCK-8 assay and cell colony formation assay. Glucose consumption, lactate production and AT...

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Autores principales:	Hongxia Zhang, Guangsheng Wu, Junjian Feng, Xiaohong Lu, Ping Liu
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	STOML2 Cell proliferation Glycolysis Multiple myeloma PAI-1 Orthopedic surgery RD701-811 Diseases of the musculoskeletal system RC925-935
Acceso en línea:	https://doaj.org/article/486d3f6c077c48968b978ec423dcb3d1
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spelling	oai:doaj.org-article:486d3f6c077c48968b978ec423dcb3d12021-11-14T12:29:35ZExpression of STOML2 promotes proliferation and glycolysis of multiple myeloma cells via upregulating PAI-110.1186/s13018-021-02819-21749-799Xhttps://doaj.org/article/486d3f6c077c48968b978ec423dcb3d12021-11-01T00:00:00Zhttps://doi.org/10.1186/s13018-021-02819-2https://doaj.org/toc/1749-799XAbstract Background This study aimed to investigate the effects of STOML2 and the relationship between STOML2 and PAI-1 in the development of multiple myeloma (MM). Methods Cell proliferation was tested using CCK-8 assay and cell colony formation assay. Glucose consumption, lactate production and ATP level were measured using commercial kits. The mRNA and protein expression were assessed using quantitative real-time polymerase chain reaction (qRT-PCR) and western blotting, respectively. Results Both mRNA and protein expression of STOML2 were upregulated in MM patients compared to healthy volunteers. CCK-8 and colony formation assays demonstrated that STOML2 silencing inhibited cell proliferation in MM cells. Knockdown of STOML2 reduced glucose consumption, lactate production and ATP/ADP ratios. STOML2 silencing by shSTOML2 led to reduced PAI-1 expression. Overexpression of PAI-1 reversed the inhibitory effects of shSTOML2 on MM cell growth. Conclusion Results from this study demonstrated that STOML2 silencing inhibits cell proliferation and glycolysis through downregulation of PAI-1 expression, suggesting a new therapeutic target for MM.Hongxia ZhangGuangsheng WuJunjian FengXiaohong LuPing LiuBMCarticleSTOML2Cell proliferationGlycolysisMultiple myelomaPAI-1Orthopedic surgeryRD701-811Diseases of the musculoskeletal systemRC925-935ENJournal of Orthopaedic Surgery and Research, Vol 16, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	STOML2 Cell proliferation Glycolysis Multiple myeloma PAI-1 Orthopedic surgery RD701-811 Diseases of the musculoskeletal system RC925-935
spellingShingle	STOML2 Cell proliferation Glycolysis Multiple myeloma PAI-1 Orthopedic surgery RD701-811 Diseases of the musculoskeletal system RC925-935 Hongxia Zhang Guangsheng Wu Junjian Feng Xiaohong Lu Ping Liu Expression of STOML2 promotes proliferation and glycolysis of multiple myeloma cells via upregulating PAI-1
description	Abstract Background This study aimed to investigate the effects of STOML2 and the relationship between STOML2 and PAI-1 in the development of multiple myeloma (MM). Methods Cell proliferation was tested using CCK-8 assay and cell colony formation assay. Glucose consumption, lactate production and ATP level were measured using commercial kits. The mRNA and protein expression were assessed using quantitative real-time polymerase chain reaction (qRT-PCR) and western blotting, respectively. Results Both mRNA and protein expression of STOML2 were upregulated in MM patients compared to healthy volunteers. CCK-8 and colony formation assays demonstrated that STOML2 silencing inhibited cell proliferation in MM cells. Knockdown of STOML2 reduced glucose consumption, lactate production and ATP/ADP ratios. STOML2 silencing by shSTOML2 led to reduced PAI-1 expression. Overexpression of PAI-1 reversed the inhibitory effects of shSTOML2 on MM cell growth. Conclusion Results from this study demonstrated that STOML2 silencing inhibits cell proliferation and glycolysis through downregulation of PAI-1 expression, suggesting a new therapeutic target for MM.
format	article
author	Hongxia Zhang Guangsheng Wu Junjian Feng Xiaohong Lu Ping Liu
author_facet	Hongxia Zhang Guangsheng Wu Junjian Feng Xiaohong Lu Ping Liu
author_sort	Hongxia Zhang
title	Expression of STOML2 promotes proliferation and glycolysis of multiple myeloma cells via upregulating PAI-1
title_short	Expression of STOML2 promotes proliferation and glycolysis of multiple myeloma cells via upregulating PAI-1
title_full	Expression of STOML2 promotes proliferation and glycolysis of multiple myeloma cells via upregulating PAI-1
title_fullStr	Expression of STOML2 promotes proliferation and glycolysis of multiple myeloma cells via upregulating PAI-1
title_full_unstemmed	Expression of STOML2 promotes proliferation and glycolysis of multiple myeloma cells via upregulating PAI-1
title_sort	expression of stoml2 promotes proliferation and glycolysis of multiple myeloma cells via upregulating pai-1
publisher	BMC
publishDate	2021
url	https://doaj.org/article/486d3f6c077c48968b978ec423dcb3d1
work_keys_str_mv	AT hongxiazhang expressionofstoml2promotesproliferationandglycolysisofmultiplemyelomacellsviaupregulatingpai1 AT guangshengwu expressionofstoml2promotesproliferationandglycolysisofmultiplemyelomacellsviaupregulatingpai1 AT junjianfeng expressionofstoml2promotesproliferationandglycolysisofmultiplemyelomacellsviaupregulatingpai1 AT xiaohonglu expressionofstoml2promotesproliferationandglycolysisofmultiplemyelomacellsviaupregulatingpai1 AT pingliu expressionofstoml2promotesproliferationandglycolysisofmultiplemyelomacellsviaupregulatingpai1
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Expression of STOML2 promotes proliferation and glycolysis of multiple myeloma cells via upregulating PAI-1

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