The Influenza A Virus M2 Protein <italic toggle="yes">trans</italic>-Complementation System Offers a Set of Tools for the Undergraduate Virology Laboratory

An authentic, hands-on experience in the laboratory is an important part of any undergraduate biology course. However, there are a limited number of mammalian virus systems that students can work with safely in an undergraduate teaching laboratory. For many systems, the risk to the students is too h...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Michael L. Grantham, Matthew F. McCown, Andrew Pekosz
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2019
Materias:	Special aspects of education LC8-6691 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/486fa1da17d3428eb45f9ba3d960d1e4
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:486fa1da17d3428eb45f9ba3d960d1e4
record_format	dspace
spelling	oai:doaj.org-article:486fa1da17d3428eb45f9ba3d960d1e42021-11-15T15:04:15ZThe Influenza A Virus M2 Protein <italic toggle="yes">trans</italic>-Complementation System Offers a Set of Tools for the Undergraduate Virology Laboratory10.1128/jmbe.v20i1.16671935-78851935-7877https://doaj.org/article/486fa1da17d3428eb45f9ba3d960d1e42019-01-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/jmbe.v20i1.1667https://doaj.org/toc/1935-7877https://doaj.org/toc/1935-7885An authentic, hands-on experience in the laboratory is an important part of any undergraduate biology course. However, there are a limited number of mammalian virus systems that students can work with safely in an undergraduate teaching laboratory. For many systems, the risk to the students is too high. The influenza A virus M2 protein trans-complementation system bridges this gap. This system consists of a virus with mutations that prevent the expression of the essential M2 protein; therefore this virus can only replicate in a cell line that provides M2 in trans. Here, we describe the use of this system to carry out hemagglutination, real-time reverse transcriptase PCR, 50% tissue culture infectious dose, and plaque assays in an undergraduate lab setting.Michael L. GranthamMatthew F. McCownAndrew PekoszAmerican Society for MicrobiologyarticleSpecial aspects of educationLC8-6691Biology (General)QH301-705.5ENJournal of Microbiology & Biology Education, Vol 20, Iss 1 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Special aspects of education LC8-6691 Biology (General) QH301-705.5
spellingShingle	Special aspects of education LC8-6691 Biology (General) QH301-705.5 Michael L. Grantham Matthew F. McCown Andrew Pekosz The Influenza A Virus M2 Protein <italic toggle="yes">trans</italic>-Complementation System Offers a Set of Tools for the Undergraduate Virology Laboratory
description	An authentic, hands-on experience in the laboratory is an important part of any undergraduate biology course. However, there are a limited number of mammalian virus systems that students can work with safely in an undergraduate teaching laboratory. For many systems, the risk to the students is too high. The influenza A virus M2 protein trans-complementation system bridges this gap. This system consists of a virus with mutations that prevent the expression of the essential M2 protein; therefore this virus can only replicate in a cell line that provides M2 in trans. Here, we describe the use of this system to carry out hemagglutination, real-time reverse transcriptase PCR, 50% tissue culture infectious dose, and plaque assays in an undergraduate lab setting.
format	article
author	Michael L. Grantham Matthew F. McCown Andrew Pekosz
author_facet	Michael L. Grantham Matthew F. McCown Andrew Pekosz
author_sort	Michael L. Grantham
title	The Influenza A Virus M2 Protein <italic toggle="yes">trans</italic>-Complementation System Offers a Set of Tools for the Undergraduate Virology Laboratory
title_short	The Influenza A Virus M2 Protein <italic toggle="yes">trans</italic>-Complementation System Offers a Set of Tools for the Undergraduate Virology Laboratory
title_full	The Influenza A Virus M2 Protein <italic toggle="yes">trans</italic>-Complementation System Offers a Set of Tools for the Undergraduate Virology Laboratory
title_fullStr	The Influenza A Virus M2 Protein <italic toggle="yes">trans</italic>-Complementation System Offers a Set of Tools for the Undergraduate Virology Laboratory
title_full_unstemmed	The Influenza A Virus M2 Protein <italic toggle="yes">trans</italic>-Complementation System Offers a Set of Tools for the Undergraduate Virology Laboratory
title_sort	influenza a virus m2 protein <italic toggle="yes">trans</italic>-complementation system offers a set of tools for the undergraduate virology laboratory
publisher	American Society for Microbiology
publishDate	2019
url	https://doaj.org/article/486fa1da17d3428eb45f9ba3d960d1e4
work_keys_str_mv	AT michaellgrantham theinfluenzaavirusm2proteinitalictoggleyestransitaliccomplementationsystemoffersasetoftoolsfortheundergraduatevirologylaboratory AT matthewfmccown theinfluenzaavirusm2proteinitalictoggleyestransitaliccomplementationsystemoffersasetoftoolsfortheundergraduatevirologylaboratory AT andrewpekosz theinfluenzaavirusm2proteinitalictoggleyestransitaliccomplementationsystemoffersasetoftoolsfortheundergraduatevirologylaboratory AT michaellgrantham influenzaavirusm2proteinitalictoggleyestransitaliccomplementationsystemoffersasetoftoolsfortheundergraduatevirologylaboratory AT matthewfmccown influenzaavirusm2proteinitalictoggleyestransitaliccomplementationsystemoffersasetoftoolsfortheundergraduatevirologylaboratory AT andrewpekosz influenzaavirusm2proteinitalictoggleyestransitaliccomplementationsystemoffersasetoftoolsfortheundergraduatevirologylaboratory
_version_	1718428248469143552

The Influenza A Virus M2 Protein <italic toggle="yes">trans</italic>-Complementation System Offers a Set of Tools for the Undergraduate Virology Laboratory

Ejemplares similares