Mesna ameliorates acute lung injury induced by intestinal ischemia–reperfusion in rats

Abstract The lung is severely affected by intestinal ischemia–reperfusion (I–R) injury. Mesna, a thiol compound, possess anti-inflammatory and antioxidant properties. We aimed in the present work to explore the potential beneficial effects of Mesna on the acute lung damage mediated by intestinal I–R...

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Autores principales: Samia Adel Abd El-Baset, Manal R. Abd El-haleem, Rehab S. Abdul-Maksoud, Asmaa A. A. Kattaia
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Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/487b5d77d1774598b66552b19c4a2c57
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spelling oai:doaj.org-article:487b5d77d1774598b66552b19c4a2c572021-12-02T16:07:04ZMesna ameliorates acute lung injury induced by intestinal ischemia–reperfusion in rats10.1038/s41598-021-92653-72045-2322https://doaj.org/article/487b5d77d1774598b66552b19c4a2c572021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92653-7https://doaj.org/toc/2045-2322Abstract The lung is severely affected by intestinal ischemia–reperfusion (I–R) injury. Mesna, a thiol compound, possess anti-inflammatory and antioxidant properties. We aimed in the present work to explore the potential beneficial effects of Mesna on the acute lung damage mediated by intestinal I–R in a rat model. Forty male adult albino rats were randomly separated into; control, intestinal I–R, Mesna I and Mesna II groups. Mesna was administered by intraperitoneal injection at a dose of 100 mg/kg, 60 min before ischemia (Mesna I) and after reperfusion (Mesna II). Arterial blood gases and total proteins in bronchoalveolar lavage (BAL) were measured. Lung tissue homogenates were utilized for biochemical assays of proinflammatory cytokines and oxidative stress markers. Lung specimens were managed for examination by light and electron microscopy. Our results revealed that Mesna attenuated the histopathological changes and apoptosis of the lung following intestinal I–R. Mesna also recovered systemic oxygenation. Mesna suppressed neutrophil infiltration (as endorsed by the reduction in MPO level), reduced ICAM-1 mRNA expression, inhibited NF-κB pathway and reduced the proinflammatory cytokines (TNF-α, IL-1β and IL-6) in the lung tissues. Mesna maintained the antioxidant profile as evidenced by the elevation of the tissue GPx and SOD and down-regulation of HSP70 immune-expressions. Accordingly, Mesna treatment can be a promising way to counteract remote injury of the lung resulted from intestinal I–R.Samia Adel Abd El-BasetManal R. Abd El-haleemRehab S. Abdul-MaksoudAsmaa A. A. KattaiaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Samia Adel Abd El-Baset
Manal R. Abd El-haleem
Rehab S. Abdul-Maksoud
Asmaa A. A. Kattaia
Mesna ameliorates acute lung injury induced by intestinal ischemia–reperfusion in rats
description Abstract The lung is severely affected by intestinal ischemia–reperfusion (I–R) injury. Mesna, a thiol compound, possess anti-inflammatory and antioxidant properties. We aimed in the present work to explore the potential beneficial effects of Mesna on the acute lung damage mediated by intestinal I–R in a rat model. Forty male adult albino rats were randomly separated into; control, intestinal I–R, Mesna I and Mesna II groups. Mesna was administered by intraperitoneal injection at a dose of 100 mg/kg, 60 min before ischemia (Mesna I) and after reperfusion (Mesna II). Arterial blood gases and total proteins in bronchoalveolar lavage (BAL) were measured. Lung tissue homogenates were utilized for biochemical assays of proinflammatory cytokines and oxidative stress markers. Lung specimens were managed for examination by light and electron microscopy. Our results revealed that Mesna attenuated the histopathological changes and apoptosis of the lung following intestinal I–R. Mesna also recovered systemic oxygenation. Mesna suppressed neutrophil infiltration (as endorsed by the reduction in MPO level), reduced ICAM-1 mRNA expression, inhibited NF-κB pathway and reduced the proinflammatory cytokines (TNF-α, IL-1β and IL-6) in the lung tissues. Mesna maintained the antioxidant profile as evidenced by the elevation of the tissue GPx and SOD and down-regulation of HSP70 immune-expressions. Accordingly, Mesna treatment can be a promising way to counteract remote injury of the lung resulted from intestinal I–R.
format article
author Samia Adel Abd El-Baset
Manal R. Abd El-haleem
Rehab S. Abdul-Maksoud
Asmaa A. A. Kattaia
author_facet Samia Adel Abd El-Baset
Manal R. Abd El-haleem
Rehab S. Abdul-Maksoud
Asmaa A. A. Kattaia
author_sort Samia Adel Abd El-Baset
title Mesna ameliorates acute lung injury induced by intestinal ischemia–reperfusion in rats
title_short Mesna ameliorates acute lung injury induced by intestinal ischemia–reperfusion in rats
title_full Mesna ameliorates acute lung injury induced by intestinal ischemia–reperfusion in rats
title_fullStr Mesna ameliorates acute lung injury induced by intestinal ischemia–reperfusion in rats
title_full_unstemmed Mesna ameliorates acute lung injury induced by intestinal ischemia–reperfusion in rats
title_sort mesna ameliorates acute lung injury induced by intestinal ischemia–reperfusion in rats
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/487b5d77d1774598b66552b19c4a2c57
work_keys_str_mv AT samiaadelabdelbaset mesnaamelioratesacutelunginjuryinducedbyintestinalischemiareperfusioninrats
AT manalrabdelhaleem mesnaamelioratesacutelunginjuryinducedbyintestinalischemiareperfusioninrats
AT rehabsabdulmaksoud mesnaamelioratesacutelunginjuryinducedbyintestinalischemiareperfusioninrats
AT asmaaaakattaia mesnaamelioratesacutelunginjuryinducedbyintestinalischemiareperfusioninrats
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