CRP immunodeposition and proteomic analysis in abdominal aortic aneurysm.

<h4>Objective</h4>The molecular mechanisms of the degeneration of the aortic wall in abdominal aortic aneurysm (AAA) are poorly understood. The monomeric form of C-reactive protein (mCRP) is deposited in damaged cardiovascular organs and aggravates the prognosis; however, it is unknown w...

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Autores principales:	Eun Na Kim, Jiyoung Yu, Joon Seo Lim, Hwangkyo Jeong, Chong Jai Kim, Jae-Sung Choi, So Ra Kim, Hee-Sung Ahn, Kyunggon Kim, Se Jin Oh
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/487c3d83a28d4e718624653383602794
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spelling	oai:doaj.org-article:487c3d83a28d4e7186246533836027942021-12-02T20:14:56ZCRP immunodeposition and proteomic analysis in abdominal aortic aneurysm.1932-620310.1371/journal.pone.0245361https://doaj.org/article/487c3d83a28d4e7186246533836027942021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0245361https://doaj.org/toc/1932-6203<h4>Objective</h4>The molecular mechanisms of the degeneration of the aortic wall in abdominal aortic aneurysm (AAA) are poorly understood. The monomeric form of C-reactive protein (mCRP) is deposited in damaged cardiovascular organs and aggravates the prognosis; however, it is unknown whether mCRP is deposited in the degenerated aorta of abdominal aortic aneurysm (AAA). We investigated whether mCRP is deposited in AAA and examined the associated pathogenic signaling pathways.<h4>Methods</h4>Twenty-four cases of AAA were analyzed and their histological features were compared according to the level of serum CRP and the degree of mCRP deposition. Proteomic analysis was performed in AAA cases with strong and diffuse CRP immunopositivity (n = 7) and those with weak, focal, and junctional CRP immunopositivity (n = 3).<h4>Results</h4>mCRP was deposited in the aortic specimens of AAA in a characteristic pattern that coincided with the lesion of the diminished elastic layer of the aortic wall. High serum CRP level was associated with stronger mCRP immunopositivity and a larger maximal diameter of aortic aneurysm. Proteomic analysis in AAA showed that multiple proteins were differentially expressed according to mCRP immunopositivity. Also, ingenuity pathway analysis showed that pathways associated with atherosclerosis, acute phase response, complement system, immune system, and coagulation were enriched in AAA cases with high mCRP immunopositivity.<h4>Conclusions</h4>AAA showed a characteristic deposition of mCRP, and multiple potentially pathologic signaling pathways were upregulated in AAA cases with strong CRP immunopositivity. mCRP and the aforementioned pathological pathways may serve as targets for managing the progression of AAA.Eun Na KimJiyoung YuJoon Seo LimHwangkyo JeongChong Jai KimJae-Sung ChoiSo Ra KimHee-Sung AhnKyunggon KimSe Jin OhPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 8, p e0245361 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Eun Na Kim Jiyoung Yu Joon Seo Lim Hwangkyo Jeong Chong Jai Kim Jae-Sung Choi So Ra Kim Hee-Sung Ahn Kyunggon Kim Se Jin Oh CRP immunodeposition and proteomic analysis in abdominal aortic aneurysm.
description	<h4>Objective</h4>The molecular mechanisms of the degeneration of the aortic wall in abdominal aortic aneurysm (AAA) are poorly understood. The monomeric form of C-reactive protein (mCRP) is deposited in damaged cardiovascular organs and aggravates the prognosis; however, it is unknown whether mCRP is deposited in the degenerated aorta of abdominal aortic aneurysm (AAA). We investigated whether mCRP is deposited in AAA and examined the associated pathogenic signaling pathways.<h4>Methods</h4>Twenty-four cases of AAA were analyzed and their histological features were compared according to the level of serum CRP and the degree of mCRP deposition. Proteomic analysis was performed in AAA cases with strong and diffuse CRP immunopositivity (n = 7) and those with weak, focal, and junctional CRP immunopositivity (n = 3).<h4>Results</h4>mCRP was deposited in the aortic specimens of AAA in a characteristic pattern that coincided with the lesion of the diminished elastic layer of the aortic wall. High serum CRP level was associated with stronger mCRP immunopositivity and a larger maximal diameter of aortic aneurysm. Proteomic analysis in AAA showed that multiple proteins were differentially expressed according to mCRP immunopositivity. Also, ingenuity pathway analysis showed that pathways associated with atherosclerosis, acute phase response, complement system, immune system, and coagulation were enriched in AAA cases with high mCRP immunopositivity.<h4>Conclusions</h4>AAA showed a characteristic deposition of mCRP, and multiple potentially pathologic signaling pathways were upregulated in AAA cases with strong CRP immunopositivity. mCRP and the aforementioned pathological pathways may serve as targets for managing the progression of AAA.
format	article
author	Eun Na Kim Jiyoung Yu Joon Seo Lim Hwangkyo Jeong Chong Jai Kim Jae-Sung Choi So Ra Kim Hee-Sung Ahn Kyunggon Kim Se Jin Oh
author_facet	Eun Na Kim Jiyoung Yu Joon Seo Lim Hwangkyo Jeong Chong Jai Kim Jae-Sung Choi So Ra Kim Hee-Sung Ahn Kyunggon Kim Se Jin Oh
author_sort	Eun Na Kim
title	CRP immunodeposition and proteomic analysis in abdominal aortic aneurysm.
title_short	CRP immunodeposition and proteomic analysis in abdominal aortic aneurysm.
title_full	CRP immunodeposition and proteomic analysis in abdominal aortic aneurysm.
title_fullStr	CRP immunodeposition and proteomic analysis in abdominal aortic aneurysm.
title_full_unstemmed	CRP immunodeposition and proteomic analysis in abdominal aortic aneurysm.
title_sort	crp immunodeposition and proteomic analysis in abdominal aortic aneurysm.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/487c3d83a28d4e718624653383602794
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_version_	1718374641225957376

CRP immunodeposition and proteomic analysis in abdominal aortic aneurysm.

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