Insight into the Antibacterial Activity of Selected Metal Nanoparticles and Alterations within the Antioxidant Defence System in Escherichia coli, Bacillus cereus and Staphylococcus epidermidis

The antimicrobial activity of nanoparticles (NPs) is a desirable feature of various products but can become problematic when NPs are released into different ecosystems, potentially endangering living microorganisms. Although there is an abundance of advanced studies on the toxicity and biological ac...

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Autores principales:	Oliwia Metryka, Daniel Wasilkowski, Agnieszka Mrozik
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	bacteria nanoparticles toxicological parameters reactive oxygen species antioxidant enzymes protein and lipid oxidation Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/487e8e4d8a6540cea1ddc9021f441025
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spelling	oai:doaj.org-article:487e8e4d8a6540cea1ddc9021f4410252021-11-11T17:15:12ZInsight into the Antibacterial Activity of Selected Metal Nanoparticles and Alterations within the Antioxidant Defence System in <i>Escherichia coli</i>, <i>Bacillus cereus</i> and <i>Staphylococcus epidermidis</i>10.3390/ijms2221118111422-00671661-6596https://doaj.org/article/487e8e4d8a6540cea1ddc9021f4410252021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11811https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067The antimicrobial activity of nanoparticles (NPs) is a desirable feature of various products but can become problematic when NPs are released into different ecosystems, potentially endangering living microorganisms. Although there is an abundance of advanced studies on the toxicity and biological activity of NPs on microorganisms, the information regarding their detailed interactions with microbial cells and the induction of oxidative stress remains incomplete. Therefore, this work aimed to develop accurate oxidation stress profiles of <i>Escherichia coli</i>, <i>Bacillus cereus</i> and <i>Staphylococcus epidermidis</i> strains treated with commercial Ag-NPs, Cu-NPs, ZnO-NPs and TiO<sub>2</sub>-NPs. The methodology used included the following determinations: toxicological parameters, reactive oxygen species (ROS), antioxidant enzymes and dehydrogenases, reduced glutathione, oxidatively modified proteins and lipid peroxidation. The toxicological studies revealed that <i>E. coli</i> was most sensitive to NPs than <i>B. cereus</i> and <i>S. epidermidis</i>. Moreover, NPs induced the generation of specific ROS in bacterial cells, causing an increase in their concentration, which further resulted in alterations in the activity of the antioxidant defence system and protein oxidation. Significant changes in dehydrogenases activity and elevated lipid peroxidation indicated a negative effect of NPs on bacterial outer layers and respiratory activity. In general, NPs were characterised by very specific nano-bio effects, depending on their physicochemical properties and the species of microorganism.Oliwia MetrykaDaniel WasilkowskiAgnieszka MrozikMDPI AGarticlebacteriananoparticlestoxicological parametersreactive oxygen speciesantioxidant enzymesprotein and lipid oxidationBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11811, p 11811 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bacteria nanoparticles toxicological parameters reactive oxygen species antioxidant enzymes protein and lipid oxidation Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	bacteria nanoparticles toxicological parameters reactive oxygen species antioxidant enzymes protein and lipid oxidation Biology (General) QH301-705.5 Chemistry QD1-999 Oliwia Metryka Daniel Wasilkowski Agnieszka Mrozik Insight into the Antibacterial Activity of Selected Metal Nanoparticles and Alterations within the Antioxidant Defence System in <i>Escherichia coli</i>, <i>Bacillus cereus</i> and <i>Staphylococcus epidermidis</i>
description	The antimicrobial activity of nanoparticles (NPs) is a desirable feature of various products but can become problematic when NPs are released into different ecosystems, potentially endangering living microorganisms. Although there is an abundance of advanced studies on the toxicity and biological activity of NPs on microorganisms, the information regarding their detailed interactions with microbial cells and the induction of oxidative stress remains incomplete. Therefore, this work aimed to develop accurate oxidation stress profiles of <i>Escherichia coli</i>, <i>Bacillus cereus</i> and <i>Staphylococcus epidermidis</i> strains treated with commercial Ag-NPs, Cu-NPs, ZnO-NPs and TiO<sub>2</sub>-NPs. The methodology used included the following determinations: toxicological parameters, reactive oxygen species (ROS), antioxidant enzymes and dehydrogenases, reduced glutathione, oxidatively modified proteins and lipid peroxidation. The toxicological studies revealed that <i>E. coli</i> was most sensitive to NPs than <i>B. cereus</i> and <i>S. epidermidis</i>. Moreover, NPs induced the generation of specific ROS in bacterial cells, causing an increase in their concentration, which further resulted in alterations in the activity of the antioxidant defence system and protein oxidation. Significant changes in dehydrogenases activity and elevated lipid peroxidation indicated a negative effect of NPs on bacterial outer layers and respiratory activity. In general, NPs were characterised by very specific nano-bio effects, depending on their physicochemical properties and the species of microorganism.
format	article
author	Oliwia Metryka Daniel Wasilkowski Agnieszka Mrozik
author_facet	Oliwia Metryka Daniel Wasilkowski Agnieszka Mrozik
author_sort	Oliwia Metryka
title	Insight into the Antibacterial Activity of Selected Metal Nanoparticles and Alterations within the Antioxidant Defence System in <i>Escherichia coli</i>, <i>Bacillus cereus</i> and <i>Staphylococcus epidermidis</i>
title_short	Insight into the Antibacterial Activity of Selected Metal Nanoparticles and Alterations within the Antioxidant Defence System in <i>Escherichia coli</i>, <i>Bacillus cereus</i> and <i>Staphylococcus epidermidis</i>
title_full	Insight into the Antibacterial Activity of Selected Metal Nanoparticles and Alterations within the Antioxidant Defence System in <i>Escherichia coli</i>, <i>Bacillus cereus</i> and <i>Staphylococcus epidermidis</i>
title_fullStr	Insight into the Antibacterial Activity of Selected Metal Nanoparticles and Alterations within the Antioxidant Defence System in <i>Escherichia coli</i>, <i>Bacillus cereus</i> and <i>Staphylococcus epidermidis</i>
title_full_unstemmed	Insight into the Antibacterial Activity of Selected Metal Nanoparticles and Alterations within the Antioxidant Defence System in <i>Escherichia coli</i>, <i>Bacillus cereus</i> and <i>Staphylococcus epidermidis</i>
title_sort	insight into the antibacterial activity of selected metal nanoparticles and alterations within the antioxidant defence system in <i>escherichia coli</i>, <i>bacillus cereus</i> and <i>staphylococcus epidermidis</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/487e8e4d8a6540cea1ddc9021f441025
work_keys_str_mv	AT oliwiametryka insightintotheantibacterialactivityofselectedmetalnanoparticlesandalterationswithintheantioxidantdefencesysteminiescherichiacoliiibacilluscereusiandistaphylococcusepidermidisi AT danielwasilkowski insightintotheantibacterialactivityofselectedmetalnanoparticlesandalterationswithintheantioxidantdefencesysteminiescherichiacoliiibacilluscereusiandistaphylococcusepidermidisi AT agnieszkamrozik insightintotheantibacterialactivityofselectedmetalnanoparticlesandalterationswithintheantioxidantdefencesysteminiescherichiacoliiibacilluscereusiandistaphylococcusepidermidisi
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Insight into the Antibacterial Activity of Selected Metal Nanoparticles and Alterations within the Antioxidant Defence System in <i>Escherichia coli</i>, <i>Bacillus cereus</i> and <i>Staphylococcus epidermidis</i>

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