A simple pressure-assisted method for MicroED specimen preparation

Micro-crystal electron diffraction (MicroED) has shown great potential for the structure determination of crystals that are too small for X-ray diffraction but MicroED sample preparation remains challenging. Here, the authors present Preassis, a pressure-assisted method for the preparation of MicroE...

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Autores principales:	Jingjing Zhao, Hongyi Xu, Hugo Lebrette, Marta Carroni, Helena Taberman, Martin Högbom, Xiaodong Zou
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/4883cb08fc2142709e055a4ec66d64e1
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id	oai:doaj.org-article:4883cb08fc2142709e055a4ec66d64e1
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spelling	oai:doaj.org-article:4883cb08fc2142709e055a4ec66d64e12021-12-02T17:08:38ZA simple pressure-assisted method for MicroED specimen preparation10.1038/s41467-021-25335-72041-1723https://doaj.org/article/4883cb08fc2142709e055a4ec66d64e12021-08-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-25335-7https://doaj.org/toc/2041-1723Micro-crystal electron diffraction (MicroED) has shown great potential for the structure determination of crystals that are too small for X-ray diffraction but MicroED sample preparation remains challenging. Here, the authors present Preassis, a pressure-assisted method for the preparation of MicroED specimens and demonstrate that Preassis can be applied to a wide range of protein crystal suspensions with low and high viscosities, as well as those with low crystal concentrations.Jingjing ZhaoHongyi XuHugo LebretteMarta CarroniHelena TabermanMartin HögbomXiaodong ZouNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Jingjing Zhao Hongyi Xu Hugo Lebrette Marta Carroni Helena Taberman Martin Högbom Xiaodong Zou A simple pressure-assisted method for MicroED specimen preparation
description	Micro-crystal electron diffraction (MicroED) has shown great potential for the structure determination of crystals that are too small for X-ray diffraction but MicroED sample preparation remains challenging. Here, the authors present Preassis, a pressure-assisted method for the preparation of MicroED specimens and demonstrate that Preassis can be applied to a wide range of protein crystal suspensions with low and high viscosities, as well as those with low crystal concentrations.
format	article
author	Jingjing Zhao Hongyi Xu Hugo Lebrette Marta Carroni Helena Taberman Martin Högbom Xiaodong Zou
author_facet	Jingjing Zhao Hongyi Xu Hugo Lebrette Marta Carroni Helena Taberman Martin Högbom Xiaodong Zou
author_sort	Jingjing Zhao
title	A simple pressure-assisted method for MicroED specimen preparation
title_short	A simple pressure-assisted method for MicroED specimen preparation
title_full	A simple pressure-assisted method for MicroED specimen preparation
title_fullStr	A simple pressure-assisted method for MicroED specimen preparation
title_full_unstemmed	A simple pressure-assisted method for MicroED specimen preparation
title_sort	simple pressure-assisted method for microed specimen preparation
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4883cb08fc2142709e055a4ec66d64e1
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A simple pressure-assisted method for MicroED specimen preparation

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