Banking Mesenchymal Stromal Cells from Umbilical Cord Tissue: Large Sample Size Analysis Reveals Consistency Between Donors

Abstract Mesenchymal stromal cells (MSCs) have emerged as candidate cells with therapeutic potential to treat different pathologies. The underlying mechanism is paracrine signaling. The cells secrete proteins that can impact inflammation, apoptosis, angiogenesis, and cell proliferation. All are impo...

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Autores principales:	Vanessa N. Raileanu, Jennifer Whiteley, Theresa Chow, Alexandra Kollara, Aisha Mohamed, Armand Keating, Ian M. Rogers
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2019
Materias:	Umbilical cord Tissue regeneration Stromal cells Somatic cell therapy Medicine (General) R5-920 Cytology QH573-671
Acceso en línea:	https://doaj.org/article/4883f86953ca4147b3b0f349cc869d62
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spelling	oai:doaj.org-article:4883f86953ca4147b3b0f349cc869d622021-11-30T19:15:37ZBanking Mesenchymal Stromal Cells from Umbilical Cord Tissue: Large Sample Size Analysis Reveals Consistency Between Donors2157-65802157-656410.1002/sctm.19-0022https://doaj.org/article/4883f86953ca4147b3b0f349cc869d622019-10-01T00:00:00Zhttps://doi.org/10.1002/sctm.19-0022https://doaj.org/toc/2157-6564https://doaj.org/toc/2157-6580Abstract Mesenchymal stromal cells (MSCs) have emerged as candidate cells with therapeutic potential to treat different pathologies. The underlying mechanism is paracrine signaling. The cells secrete proteins that can impact inflammation, apoptosis, angiogenesis, and cell proliferation. All are important in wound healing and tissue regeneration. Although the bone marrow has been the most widely used source of MSCs, umbilical cord tissue (CT) presents a source that is just starting to be used in the clinic, yet can be obtained with more ease and easily stored. Here, we characterize CT‐MSCs obtained from multiple donors by analyzing cell surface proteins, differentiation capacity, and proteome profile. Analysis of low, medium, and high passage cells indicates that the morphology and proliferation rate stay constant and with the exception of cluster of differentiation (CD) 105 at late passage, there are no changes in the cell surface protein characteristics, indicating the population does not change with passage. TNF‐stimulated gene 6 protein was measured in a subset of samples and variable expression was observed, but this did not impact the ability of the cells to enhance skin regeneration. In conclusion, CT‐MSC represents a consistent, easily accessible source of cells for cell therapy. Stem Cells Translational Medicine 2019;8:1041–1054Vanessa N. RaileanuJennifer WhiteleyTheresa ChowAlexandra KollaraAisha MohamedArmand KeatingIan M. RogersWileyarticleUmbilical cordTissue regenerationStromal cellsSomatic cell therapyMedicine (General)R5-920CytologyQH573-671ENStem Cells Translational Medicine, Vol 8, Iss 10, Pp 1041-1054 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Umbilical cord Tissue regeneration Stromal cells Somatic cell therapy Medicine (General) R5-920 Cytology QH573-671
spellingShingle	Umbilical cord Tissue regeneration Stromal cells Somatic cell therapy Medicine (General) R5-920 Cytology QH573-671 Vanessa N. Raileanu Jennifer Whiteley Theresa Chow Alexandra Kollara Aisha Mohamed Armand Keating Ian M. Rogers Banking Mesenchymal Stromal Cells from Umbilical Cord Tissue: Large Sample Size Analysis Reveals Consistency Between Donors
description	Abstract Mesenchymal stromal cells (MSCs) have emerged as candidate cells with therapeutic potential to treat different pathologies. The underlying mechanism is paracrine signaling. The cells secrete proteins that can impact inflammation, apoptosis, angiogenesis, and cell proliferation. All are important in wound healing and tissue regeneration. Although the bone marrow has been the most widely used source of MSCs, umbilical cord tissue (CT) presents a source that is just starting to be used in the clinic, yet can be obtained with more ease and easily stored. Here, we characterize CT‐MSCs obtained from multiple donors by analyzing cell surface proteins, differentiation capacity, and proteome profile. Analysis of low, medium, and high passage cells indicates that the morphology and proliferation rate stay constant and with the exception of cluster of differentiation (CD) 105 at late passage, there are no changes in the cell surface protein characteristics, indicating the population does not change with passage. TNF‐stimulated gene 6 protein was measured in a subset of samples and variable expression was observed, but this did not impact the ability of the cells to enhance skin regeneration. In conclusion, CT‐MSC represents a consistent, easily accessible source of cells for cell therapy. Stem Cells Translational Medicine 2019;8:1041–1054
format	article
author	Vanessa N. Raileanu Jennifer Whiteley Theresa Chow Alexandra Kollara Aisha Mohamed Armand Keating Ian M. Rogers
author_facet	Vanessa N. Raileanu Jennifer Whiteley Theresa Chow Alexandra Kollara Aisha Mohamed Armand Keating Ian M. Rogers
author_sort	Vanessa N. Raileanu
title	Banking Mesenchymal Stromal Cells from Umbilical Cord Tissue: Large Sample Size Analysis Reveals Consistency Between Donors
title_short	Banking Mesenchymal Stromal Cells from Umbilical Cord Tissue: Large Sample Size Analysis Reveals Consistency Between Donors
title_full	Banking Mesenchymal Stromal Cells from Umbilical Cord Tissue: Large Sample Size Analysis Reveals Consistency Between Donors
title_fullStr	Banking Mesenchymal Stromal Cells from Umbilical Cord Tissue: Large Sample Size Analysis Reveals Consistency Between Donors
title_full_unstemmed	Banking Mesenchymal Stromal Cells from Umbilical Cord Tissue: Large Sample Size Analysis Reveals Consistency Between Donors
title_sort	banking mesenchymal stromal cells from umbilical cord tissue: large sample size analysis reveals consistency between donors
publisher	Wiley
publishDate	2019
url	https://doaj.org/article/4883f86953ca4147b3b0f349cc869d62
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Banking Mesenchymal Stromal Cells from Umbilical Cord Tissue: Large Sample Size Analysis Reveals Consistency Between Donors

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