Contribution of purified soybean trypsin inhibitor and exogenous protease to endogenous amino acid losses and mineral digestibility

ABSTRACT: The primary objective of the current study was to evaluate the impact of trypsin inhibitor (TI) and exogenous protease supplementation on endogenous loss of amino acids (AA) in broiler chickens. A total of 384 Cobb-500 broiler chicks were allocated to 4 nitrogen-free diets, each with 8 rep...

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Autores principales:	A.S. Aderibigbe, A.J. Cowieson, K.M. Ajuwon, O. Adeola
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	amino acid endogenous loss mineral protease trypsin inhibitor Animal culture SF1-1100
Acceso en línea:	https://doaj.org/article/488603d99a17419580edb4be41f04c4b
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spelling	oai:doaj.org-article:488603d99a17419580edb4be41f04c4b2021-11-24T04:22:19ZContribution of purified soybean trypsin inhibitor and exogenous protease to endogenous amino acid losses and mineral digestibility0032-579110.1016/j.psj.2021.101486https://doaj.org/article/488603d99a17419580edb4be41f04c4b2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0032579121005083https://doaj.org/toc/0032-5791ABSTRACT: The primary objective of the current study was to evaluate the impact of trypsin inhibitor (TI) and exogenous protease supplementation on endogenous loss of amino acids (AA) in broiler chickens. A total of 384 Cobb-500 broiler chicks were allocated to 4 nitrogen-free diets, each with 8 replicate cages and 12 birds per replicate. The diets were arranged as a 2 × 2 factorial with factors being dietary TI (0 or 8,000 TIU/g) and exogenous protease (0 or 15,000 PROT/kg). Desired dietary TI concentration was achieved by addition of commercially available, purified soybean TI. There was no effect of TI or exogenous protease or their interaction on growth performance of birds. However, the endogenous loss of nitrogen (N) and all AA increased (P < 0.05) due to dietary TI concentration except for Cys. The increase in endogenous AA due to TI ranged from 17% for Thr to 52.2% for Trp. Exogenous protease had no effect on endogenous loss of N and all AA. There was no effect of TI or exogenous protease or their interaction on the AID of P, however AID of Ca, Fe, Mg, Mn, and Cu was reduced (P < 0.05) due to dietary TI. The AID of Cu (P < 0.01) and K (P < 0.05) improved with exogenous protease supplementation. Significant interactions (P < 0.05) between exogenous protease and TI existed for Zn, Mg, Cu, and Na. The concentration (g/kg DM intake) of crude mucin and sialic acid increased (P < 0.05) with increased dietary TI. Decreased trypsin (P < 0.001) and increased chymotrypsin (P < 0.001) activity in the pancreas were observed as a result of exogenous protease supplementation. In conclusion, the current study showed that TI increases the endogenous loss of AA and reduced the digestibility of minerals in broiler chickens. Furthermore, exogenous protease did not affect endogenous AA flow, irrespective of added purified dietary TI.A.S. AderibigbeA.J. CowiesonK.M. AjuwonO. AdeolaElsevierarticleamino acidendogenous lossmineralproteasetrypsin inhibitorAnimal cultureSF1-1100ENPoultry Science, Vol 100, Iss 12, Pp 101486- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	amino acid endogenous loss mineral protease trypsin inhibitor Animal culture SF1-1100
spellingShingle	amino acid endogenous loss mineral protease trypsin inhibitor Animal culture SF1-1100 A.S. Aderibigbe A.J. Cowieson K.M. Ajuwon O. Adeola Contribution of purified soybean trypsin inhibitor and exogenous protease to endogenous amino acid losses and mineral digestibility
description	ABSTRACT: The primary objective of the current study was to evaluate the impact of trypsin inhibitor (TI) and exogenous protease supplementation on endogenous loss of amino acids (AA) in broiler chickens. A total of 384 Cobb-500 broiler chicks were allocated to 4 nitrogen-free diets, each with 8 replicate cages and 12 birds per replicate. The diets were arranged as a 2 × 2 factorial with factors being dietary TI (0 or 8,000 TIU/g) and exogenous protease (0 or 15,000 PROT/kg). Desired dietary TI concentration was achieved by addition of commercially available, purified soybean TI. There was no effect of TI or exogenous protease or their interaction on growth performance of birds. However, the endogenous loss of nitrogen (N) and all AA increased (P < 0.05) due to dietary TI concentration except for Cys. The increase in endogenous AA due to TI ranged from 17% for Thr to 52.2% for Trp. Exogenous protease had no effect on endogenous loss of N and all AA. There was no effect of TI or exogenous protease or their interaction on the AID of P, however AID of Ca, Fe, Mg, Mn, and Cu was reduced (P < 0.05) due to dietary TI. The AID of Cu (P < 0.01) and K (P < 0.05) improved with exogenous protease supplementation. Significant interactions (P < 0.05) between exogenous protease and TI existed for Zn, Mg, Cu, and Na. The concentration (g/kg DM intake) of crude mucin and sialic acid increased (P < 0.05) with increased dietary TI. Decreased trypsin (P < 0.001) and increased chymotrypsin (P < 0.001) activity in the pancreas were observed as a result of exogenous protease supplementation. In conclusion, the current study showed that TI increases the endogenous loss of AA and reduced the digestibility of minerals in broiler chickens. Furthermore, exogenous protease did not affect endogenous AA flow, irrespective of added purified dietary TI.
format	article
author	A.S. Aderibigbe A.J. Cowieson K.M. Ajuwon O. Adeola
author_facet	A.S. Aderibigbe A.J. Cowieson K.M. Ajuwon O. Adeola
author_sort	A.S. Aderibigbe
title	Contribution of purified soybean trypsin inhibitor and exogenous protease to endogenous amino acid losses and mineral digestibility
title_short	Contribution of purified soybean trypsin inhibitor and exogenous protease to endogenous amino acid losses and mineral digestibility
title_full	Contribution of purified soybean trypsin inhibitor and exogenous protease to endogenous amino acid losses and mineral digestibility
title_fullStr	Contribution of purified soybean trypsin inhibitor and exogenous protease to endogenous amino acid losses and mineral digestibility
title_full_unstemmed	Contribution of purified soybean trypsin inhibitor and exogenous protease to endogenous amino acid losses and mineral digestibility
title_sort	contribution of purified soybean trypsin inhibitor and exogenous protease to endogenous amino acid losses and mineral digestibility
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/488603d99a17419580edb4be41f04c4b
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Contribution of purified soybean trypsin inhibitor and exogenous protease to endogenous amino acid losses and mineral digestibility

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