Experimental design in HPLC separation of pharmaceuticals

Design of Experiments (DoE) is an indispensable tool in contemporary drug analysis as it simultaneously balances a number of chromatographic parameters to ensure optimal separation in High Pressure Liquid Chromatography (HPLC). This manuscript briefly outlines the theoretical background of the DOE a...

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Autores principales:	Stojanović Jevrem, Krmar Jovana, Protić Ana, Svrkota Bojana, Đajić Nevena, Otašević Biljana
Formato:	article
Lenguaje:	SR
Publicado:	Pharmaceutical Association of Serbia, Belgrade, Serbia 2021
Materias:	design of experiments drug analysis high pressure liquid chromatography analytical method development and validation Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/488790f20c7545028ffc12eb431abfd7
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spelling	oai:doaj.org-article:488790f20c7545028ffc12eb431abfd72021-12-05T18:01:35ZExperimental design in HPLC separation of pharmaceuticals0004-19632217-876710.5937/arhfarm71-32480https://doaj.org/article/488790f20c7545028ffc12eb431abfd72021-01-01T00:00:00Zhttps://scindeks-clanci.ceon.rs/data/pdf/0004-1963/2021/0004-19632104279S.pdfhttps://doaj.org/toc/0004-1963https://doaj.org/toc/2217-8767Design of Experiments (DoE) is an indispensable tool in contemporary drug analysis as it simultaneously balances a number of chromatographic parameters to ensure optimal separation in High Pressure Liquid Chromatography (HPLC). This manuscript briefly outlines the theoretical background of the DOE and provides step-by-step instruction for its implementation in HPLC pharmaceutical practice. It particularly discusses the classification of various design types and their possibilities to rationalize the different stages of HPLC method development workflow, such as the selection of the most influential factors, factors optimization and assessment of the method robustness. Additionally, the application of the DOE-based Analytical Quality by Design (AQbD) concept in the LC method development has been summarized. Recent achievements in the use of DOE in the development of stability-indicating LC and hyphenated LC-MS methods have also been briefly reported. Performing of Quantitative structure retention relationship (QSRR) study enhanced with DOE-based data collection was recomended as a future perspective in description of retention in HPLC system.Stojanović JevremKrmar JovanaProtić AnaSvrkota BojanaĐajić NevenaOtašević BiljanaPharmaceutical Association of Serbia, Belgrade, Serbiaarticledesign of experimentsdrug analysishigh pressure liquid chromatographyanalytical method development and validationPharmacy and materia medicaRS1-441SRArhiv za farmaciju, Vol 71, Iss 4, Pp 279-301 (2021)
institution	DOAJ
collection	DOAJ
language	SR
topic	design of experiments drug analysis high pressure liquid chromatography analytical method development and validation Pharmacy and materia medica RS1-441
spellingShingle	design of experiments drug analysis high pressure liquid chromatography analytical method development and validation Pharmacy and materia medica RS1-441 Stojanović Jevrem Krmar Jovana Protić Ana Svrkota Bojana Đajić Nevena Otašević Biljana Experimental design in HPLC separation of pharmaceuticals
description	Design of Experiments (DoE) is an indispensable tool in contemporary drug analysis as it simultaneously balances a number of chromatographic parameters to ensure optimal separation in High Pressure Liquid Chromatography (HPLC). This manuscript briefly outlines the theoretical background of the DOE and provides step-by-step instruction for its implementation in HPLC pharmaceutical practice. It particularly discusses the classification of various design types and their possibilities to rationalize the different stages of HPLC method development workflow, such as the selection of the most influential factors, factors optimization and assessment of the method robustness. Additionally, the application of the DOE-based Analytical Quality by Design (AQbD) concept in the LC method development has been summarized. Recent achievements in the use of DOE in the development of stability-indicating LC and hyphenated LC-MS methods have also been briefly reported. Performing of Quantitative structure retention relationship (QSRR) study enhanced with DOE-based data collection was recomended as a future perspective in description of retention in HPLC system.
format	article
author	Stojanović Jevrem Krmar Jovana Protić Ana Svrkota Bojana Đajić Nevena Otašević Biljana
author_facet	Stojanović Jevrem Krmar Jovana Protić Ana Svrkota Bojana Đajić Nevena Otašević Biljana
author_sort	Stojanović Jevrem
title	Experimental design in HPLC separation of pharmaceuticals
title_short	Experimental design in HPLC separation of pharmaceuticals
title_full	Experimental design in HPLC separation of pharmaceuticals
title_fullStr	Experimental design in HPLC separation of pharmaceuticals
title_full_unstemmed	Experimental design in HPLC separation of pharmaceuticals
title_sort	experimental design in hplc separation of pharmaceuticals
publisher	Pharmaceutical Association of Serbia, Belgrade, Serbia
publishDate	2021
url	https://doaj.org/article/488790f20c7545028ffc12eb431abfd7
work_keys_str_mv	AT stojanovicjevrem experimentaldesigninhplcseparationofpharmaceuticals AT krmarjovana experimentaldesigninhplcseparationofpharmaceuticals AT proticana experimentaldesigninhplcseparationofpharmaceuticals AT svrkotabojana experimentaldesigninhplcseparationofpharmaceuticals AT đajicnevena experimentaldesigninhplcseparationofpharmaceuticals AT otasevicbiljana experimentaldesigninhplcseparationofpharmaceuticals
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Experimental design in HPLC separation of pharmaceuticals

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