Genome analysis of the metabolically versatile Pseudomonas umsongensis GO16: the genetic basis for PET monomer upcycling into polyhydroxyalkanoates

Summary The throwaway culture related to the single‐use materials such as polyethylene terephthalate (PET) has created a major environmental concern. Recycling of PET waste into biodegradable plastic polyhydroxyalkanoate (PHA) creates an opportunity to improve resource efficiency and contribute to a...

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Autores principales:	Tanja Narancic, Manuel Salvador, Graham M. Hughes, Niall Beagan, Umar Abdulmutalib, Shane T. Kenny, Huihai Wu, Marta Saccomanno, Jounghyun Um, Kevin E. O'Connor, José I. Jiménez
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/4889eec40a434ce9a0e39a152c53676c
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spelling	oai:doaj.org-article:4889eec40a434ce9a0e39a152c53676c2021-11-18T15:39:52ZGenome analysis of the metabolically versatile Pseudomonas umsongensis GO16: the genetic basis for PET monomer upcycling into polyhydroxyalkanoates1751-791510.1111/1751-7915.13712https://doaj.org/article/4889eec40a434ce9a0e39a152c53676c2021-11-01T00:00:00Zhttps://doi.org/10.1111/1751-7915.13712https://doaj.org/toc/1751-7915Summary The throwaway culture related to the single‐use materials such as polyethylene terephthalate (PET) has created a major environmental concern. Recycling of PET waste into biodegradable plastic polyhydroxyalkanoate (PHA) creates an opportunity to improve resource efficiency and contribute to a circular economy. We sequenced the genome of Pseudomonas umsongensis GO16 previously shown to convert PET‐derived terephthalic acid (TA) into PHA and performed an in‐depth genome analysis. GO16 can degrade a range of aromatic substrates in addition to TA, due to the presence of a catabolic plasmid pENK22. The genetic complement required for the degradation of TA via protocatechuate was identified and its functionality was confirmed by transferring the tph operon into Pseudomonas putida KT2440, which is unable to utilize TA naturally. We also identified the genes involved in ethylene glycol (EG) metabolism, the second PET monomer, and validated the capacity of GO16 to use EG as a sole source of carbon and energy. Moreover, GO16 possesses genes for the synthesis of both medium and short chain length PHA and we have demonstrated the capacity of the strain to convert mixed TA and EG into PHA. The metabolic versatility of GO16 highlights the potential of this organism for biotransformations using PET waste as a feedstock.Tanja NarancicManuel SalvadorGraham M. HughesNiall BeaganUmar AbdulmutalibShane T. KennyHuihai WuMarta SaccomannoJounghyun UmKevin E. O'ConnorJosé I. JiménezWileyarticleBiotechnologyTP248.13-248.65ENMicrobial Biotechnology, Vol 14, Iss 6, Pp 2463-2480 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biotechnology TP248.13-248.65
spellingShingle	Biotechnology TP248.13-248.65 Tanja Narancic Manuel Salvador Graham M. Hughes Niall Beagan Umar Abdulmutalib Shane T. Kenny Huihai Wu Marta Saccomanno Jounghyun Um Kevin E. O'Connor José I. Jiménez Genome analysis of the metabolically versatile Pseudomonas umsongensis GO16: the genetic basis for PET monomer upcycling into polyhydroxyalkanoates
description	Summary The throwaway culture related to the single‐use materials such as polyethylene terephthalate (PET) has created a major environmental concern. Recycling of PET waste into biodegradable plastic polyhydroxyalkanoate (PHA) creates an opportunity to improve resource efficiency and contribute to a circular economy. We sequenced the genome of Pseudomonas umsongensis GO16 previously shown to convert PET‐derived terephthalic acid (TA) into PHA and performed an in‐depth genome analysis. GO16 can degrade a range of aromatic substrates in addition to TA, due to the presence of a catabolic plasmid pENK22. The genetic complement required for the degradation of TA via protocatechuate was identified and its functionality was confirmed by transferring the tph operon into Pseudomonas putida KT2440, which is unable to utilize TA naturally. We also identified the genes involved in ethylene glycol (EG) metabolism, the second PET monomer, and validated the capacity of GO16 to use EG as a sole source of carbon and energy. Moreover, GO16 possesses genes for the synthesis of both medium and short chain length PHA and we have demonstrated the capacity of the strain to convert mixed TA and EG into PHA. The metabolic versatility of GO16 highlights the potential of this organism for biotransformations using PET waste as a feedstock.
format	article
author	Tanja Narancic Manuel Salvador Graham M. Hughes Niall Beagan Umar Abdulmutalib Shane T. Kenny Huihai Wu Marta Saccomanno Jounghyun Um Kevin E. O'Connor José I. Jiménez
author_facet	Tanja Narancic Manuel Salvador Graham M. Hughes Niall Beagan Umar Abdulmutalib Shane T. Kenny Huihai Wu Marta Saccomanno Jounghyun Um Kevin E. O'Connor José I. Jiménez
author_sort	Tanja Narancic
title	Genome analysis of the metabolically versatile Pseudomonas umsongensis GO16: the genetic basis for PET monomer upcycling into polyhydroxyalkanoates
title_short	Genome analysis of the metabolically versatile Pseudomonas umsongensis GO16: the genetic basis for PET monomer upcycling into polyhydroxyalkanoates
title_full	Genome analysis of the metabolically versatile Pseudomonas umsongensis GO16: the genetic basis for PET monomer upcycling into polyhydroxyalkanoates
title_fullStr	Genome analysis of the metabolically versatile Pseudomonas umsongensis GO16: the genetic basis for PET monomer upcycling into polyhydroxyalkanoates
title_full_unstemmed	Genome analysis of the metabolically versatile Pseudomonas umsongensis GO16: the genetic basis for PET monomer upcycling into polyhydroxyalkanoates
title_sort	genome analysis of the metabolically versatile pseudomonas umsongensis go16: the genetic basis for pet monomer upcycling into polyhydroxyalkanoates
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/4889eec40a434ce9a0e39a152c53676c
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Genome analysis of the metabolically versatile Pseudomonas umsongensis GO16: the genetic basis for PET monomer upcycling into polyhydroxyalkanoates

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