Productive infection of bovine papillomavirus type 2 in the urothelial cells of naturally occurring urinary bladder tumors in cattle and water buffaloes.

<h4>Background</h4>Papillomaviruses (PVs) are highly epitheliotropic as they usually establish productive infections within squamous epithelia of the skin, the anogenital tract and the oral cavity. In this study, early (E) and late (L) protein expression of bovine papillomavirus type 2 (...

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Autores principales:	Sante Roperto, Valeria Russo, Ayhan Ozkul, Annunziata Corteggio, Aylin Sepici-Dincel, Cornel Catoi, Iolanda Esposito, Marita G Riccardi, Chiara Urraro, Roberta Lucà, Dora M Ceccarelli, Michele Longo, Franco Roperto
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/488a44bfac7c4a67827d7cae66b2424d
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spelling	oai:doaj.org-article:488a44bfac7c4a67827d7cae66b2424d2021-11-18T07:46:43ZProductive infection of bovine papillomavirus type 2 in the urothelial cells of naturally occurring urinary bladder tumors in cattle and water buffaloes.1932-620310.1371/journal.pone.0062227https://doaj.org/article/488a44bfac7c4a67827d7cae66b2424d2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23667460/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Papillomaviruses (PVs) are highly epitheliotropic as they usually establish productive infections within squamous epithelia of the skin, the anogenital tract and the oral cavity. In this study, early (E) and late (L) protein expression of bovine papillomavirus type 2 (BPV-2) in the urothelium of the urinary bladder is described in cows and water buffaloes suffering from naturally occurring papillomavirus-associated urothelial bladder tumors.<h4>Methods and findings</h4>E5 protein, the major oncoprotein of the BPV-2, was detected in all tumors. L1 DNA was amplified by PCR, cloned and sequenced and confirmed to be L1 DNA. The major capsid protein, L1, believed to be only expressed in productive papillomavirus infection was detected by Western blot analysis. Immunohistochemical investigations confirmed the presence of L1 protein both in the cytoplasm and nuclei of cells of the neoplastic urothelium. Finally, the early protein E2, required for viral DNA replication and known to be a pivotal factor for both productive and persistent infection, was detected by Western blot and immunohistochemically. Electron microscopic investigations detected electron dense particles, the shape and size of which are consistent with submicroscopic features of viral particles, in nuclei of neoplastic urothelium.<h4>Conclusion</h4>This study shows that both active and productive infections by BPV-2 in the urothelium of the bovine and bubaline urinary bladder can occur in vivo.Sante RopertoValeria RussoAyhan OzkulAnnunziata CorteggioAylin Sepici-DincelCornel CatoiIolanda EspositoMarita G RiccardiChiara UrraroRoberta LucàDora M CeccarelliMichele LongoFranco RopertoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 5, p e62227 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sante Roperto Valeria Russo Ayhan Ozkul Annunziata Corteggio Aylin Sepici-Dincel Cornel Catoi Iolanda Esposito Marita G Riccardi Chiara Urraro Roberta Lucà Dora M Ceccarelli Michele Longo Franco Roperto Productive infection of bovine papillomavirus type 2 in the urothelial cells of naturally occurring urinary bladder tumors in cattle and water buffaloes.
description	<h4>Background</h4>Papillomaviruses (PVs) are highly epitheliotropic as they usually establish productive infections within squamous epithelia of the skin, the anogenital tract and the oral cavity. In this study, early (E) and late (L) protein expression of bovine papillomavirus type 2 (BPV-2) in the urothelium of the urinary bladder is described in cows and water buffaloes suffering from naturally occurring papillomavirus-associated urothelial bladder tumors.<h4>Methods and findings</h4>E5 protein, the major oncoprotein of the BPV-2, was detected in all tumors. L1 DNA was amplified by PCR, cloned and sequenced and confirmed to be L1 DNA. The major capsid protein, L1, believed to be only expressed in productive papillomavirus infection was detected by Western blot analysis. Immunohistochemical investigations confirmed the presence of L1 protein both in the cytoplasm and nuclei of cells of the neoplastic urothelium. Finally, the early protein E2, required for viral DNA replication and known to be a pivotal factor for both productive and persistent infection, was detected by Western blot and immunohistochemically. Electron microscopic investigations detected electron dense particles, the shape and size of which are consistent with submicroscopic features of viral particles, in nuclei of neoplastic urothelium.<h4>Conclusion</h4>This study shows that both active and productive infections by BPV-2 in the urothelium of the bovine and bubaline urinary bladder can occur in vivo.
format	article
author	Sante Roperto Valeria Russo Ayhan Ozkul Annunziata Corteggio Aylin Sepici-Dincel Cornel Catoi Iolanda Esposito Marita G Riccardi Chiara Urraro Roberta Lucà Dora M Ceccarelli Michele Longo Franco Roperto
author_facet	Sante Roperto Valeria Russo Ayhan Ozkul Annunziata Corteggio Aylin Sepici-Dincel Cornel Catoi Iolanda Esposito Marita G Riccardi Chiara Urraro Roberta Lucà Dora M Ceccarelli Michele Longo Franco Roperto
author_sort	Sante Roperto
title	Productive infection of bovine papillomavirus type 2 in the urothelial cells of naturally occurring urinary bladder tumors in cattle and water buffaloes.
title_short	Productive infection of bovine papillomavirus type 2 in the urothelial cells of naturally occurring urinary bladder tumors in cattle and water buffaloes.
title_full	Productive infection of bovine papillomavirus type 2 in the urothelial cells of naturally occurring urinary bladder tumors in cattle and water buffaloes.
title_fullStr	Productive infection of bovine papillomavirus type 2 in the urothelial cells of naturally occurring urinary bladder tumors in cattle and water buffaloes.
title_full_unstemmed	Productive infection of bovine papillomavirus type 2 in the urothelial cells of naturally occurring urinary bladder tumors in cattle and water buffaloes.
title_sort	productive infection of bovine papillomavirus type 2 in the urothelial cells of naturally occurring urinary bladder tumors in cattle and water buffaloes.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/488a44bfac7c4a67827d7cae66b2424d
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Productive infection of bovine papillomavirus type 2 in the urothelial cells of naturally occurring urinary bladder tumors in cattle and water buffaloes.

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