Internalized Nanoceria Modify the Radiation-Sensitivity Profile of MDA MB231 Breast Carcinoma Cells

Owing to its unique redox properties, cerium oxide (nanoceria) nanoparticles have been shown to confer either radiosensitization or radioprotection to human cells. We investigated nanoceria’s ability to modify cellular health and reactive oxygen species (ROS) at various absorbed doses (Gray) of ioni...

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Autores principales:	Emory Bibb, Noura Alajlan, Saad Alsuwailem, Benjamin Mitchell, Amy Brady, Muhammad Maqbool, Remo George
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	radiosensitivity radiation protection cerium oxide nanoparticles MDA MB231 cells macropinocytosis macropinosomes Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/488cc2eaa4aa4a0eaedccaa55624e5b9
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spelling	oai:doaj.org-article:488cc2eaa4aa4a0eaedccaa55624e5b92021-11-25T16:47:27ZInternalized Nanoceria Modify the Radiation-Sensitivity Profile of MDA MB231 Breast Carcinoma Cells10.3390/biology101111482079-7737https://doaj.org/article/488cc2eaa4aa4a0eaedccaa55624e5b92021-11-01T00:00:00Zhttps://www.mdpi.com/2079-7737/10/11/1148https://doaj.org/toc/2079-7737Owing to its unique redox properties, cerium oxide (nanoceria) nanoparticles have been shown to confer either radiosensitization or radioprotection to human cells. We investigated nanoceria’s ability to modify cellular health and reactive oxygen species (ROS) at various absorbed doses (Gray) of ionizing radiation in MDA-MB231 breast carcinoma cells. We used transmission electron microscopy to visualize the uptake and compartmental localization of nanoceria within cells at various treatment concentrations. The effects on apoptosis and other cellular health parameters were assessed using confocal fluorescence imaging and flow cytometry without and with various absorbed doses of ionizing radiation, along with intracellular ROS levels. Our results showed that nanoceria were taken up into cells mainly by macropinocytosis and segregated into concentration-dependent large aggregates in macropinosomes. Confocal imaging and flow cytometry data showed an overall decrease in apoptotic cell populations in proportion to increasing nanoparticle concentrations. This increase in cellular health was observed with a corresponding reduction in ROS at all tested absorbed doses. Moreover, this effect appeared pronounced at lower doses compared to unirradiated or untreated populations. In conclusion, internalized nanoceria confers radioprotection with a corresponding decrease in ROS in MDA-MB231 cells, and this property confers significant perils and opportunities when utilized in the context of radiotherapy.Emory BibbNoura AlajlanSaad AlsuwailemBenjamin MitchellAmy BradyMuhammad MaqboolRemo GeorgeMDPI AGarticleradiosensitivityradiation protectioncerium oxide nanoparticlesMDA MB231 cellsmacropinocytosismacropinosomesBiology (General)QH301-705.5ENBiology, Vol 10, Iss 1148, p 1148 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	radiosensitivity radiation protection cerium oxide nanoparticles MDA MB231 cells macropinocytosis macropinosomes Biology (General) QH301-705.5
spellingShingle	radiosensitivity radiation protection cerium oxide nanoparticles MDA MB231 cells macropinocytosis macropinosomes Biology (General) QH301-705.5 Emory Bibb Noura Alajlan Saad Alsuwailem Benjamin Mitchell Amy Brady Muhammad Maqbool Remo George Internalized Nanoceria Modify the Radiation-Sensitivity Profile of MDA MB231 Breast Carcinoma Cells
description	Owing to its unique redox properties, cerium oxide (nanoceria) nanoparticles have been shown to confer either radiosensitization or radioprotection to human cells. We investigated nanoceria’s ability to modify cellular health and reactive oxygen species (ROS) at various absorbed doses (Gray) of ionizing radiation in MDA-MB231 breast carcinoma cells. We used transmission electron microscopy to visualize the uptake and compartmental localization of nanoceria within cells at various treatment concentrations. The effects on apoptosis and other cellular health parameters were assessed using confocal fluorescence imaging and flow cytometry without and with various absorbed doses of ionizing radiation, along with intracellular ROS levels. Our results showed that nanoceria were taken up into cells mainly by macropinocytosis and segregated into concentration-dependent large aggregates in macropinosomes. Confocal imaging and flow cytometry data showed an overall decrease in apoptotic cell populations in proportion to increasing nanoparticle concentrations. This increase in cellular health was observed with a corresponding reduction in ROS at all tested absorbed doses. Moreover, this effect appeared pronounced at lower doses compared to unirradiated or untreated populations. In conclusion, internalized nanoceria confers radioprotection with a corresponding decrease in ROS in MDA-MB231 cells, and this property confers significant perils and opportunities when utilized in the context of radiotherapy.
format	article
author	Emory Bibb Noura Alajlan Saad Alsuwailem Benjamin Mitchell Amy Brady Muhammad Maqbool Remo George
author_facet	Emory Bibb Noura Alajlan Saad Alsuwailem Benjamin Mitchell Amy Brady Muhammad Maqbool Remo George
author_sort	Emory Bibb
title	Internalized Nanoceria Modify the Radiation-Sensitivity Profile of MDA MB231 Breast Carcinoma Cells
title_short	Internalized Nanoceria Modify the Radiation-Sensitivity Profile of MDA MB231 Breast Carcinoma Cells
title_full	Internalized Nanoceria Modify the Radiation-Sensitivity Profile of MDA MB231 Breast Carcinoma Cells
title_fullStr	Internalized Nanoceria Modify the Radiation-Sensitivity Profile of MDA MB231 Breast Carcinoma Cells
title_full_unstemmed	Internalized Nanoceria Modify the Radiation-Sensitivity Profile of MDA MB231 Breast Carcinoma Cells
title_sort	internalized nanoceria modify the radiation-sensitivity profile of mda mb231 breast carcinoma cells
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/488cc2eaa4aa4a0eaedccaa55624e5b9
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Internalized Nanoceria Modify the Radiation-Sensitivity Profile of MDA MB231 Breast Carcinoma Cells

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