Adamdec1, Ednrb and Ptgs1/Cox1, inflammation genes upregulated in the intestinal mucosa of obese rats, are downregulated by three probiotic strains

Abstract We have previously reported that administration of Lactobacillus paracasei CNCM I-4034, Bifidobacterium breve CNCM I-4035 and Lactobacillus rhamnosus CNCM I-4036 to obese Zucker-Lepr fa/fa rats attenuates liver steatosis and exerts anti-inflammatory effects. The goal of the present work was...

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Autores principales:	Julio Plaza-Díaz, Cándido Robles-Sánchez, Francisco Abadía-Molina, Virginia Morón-Calvente, María José Sáez-Lara, Alfonso Ruiz-Bravo, María Jiménez-Valera, Ángel Gil, Carolina Gómez-Llorente, Luis Fontana
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4897946dd49e4222819f7c9d6c31fdd4
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spelling	oai:doaj.org-article:4897946dd49e4222819f7c9d6c31fdd42021-12-02T11:53:10ZAdamdec1, Ednrb and Ptgs1/Cox1, inflammation genes upregulated in the intestinal mucosa of obese rats, are downregulated by three probiotic strains10.1038/s41598-017-02203-32045-2322https://doaj.org/article/4897946dd49e4222819f7c9d6c31fdd42017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02203-3https://doaj.org/toc/2045-2322Abstract We have previously reported that administration of Lactobacillus paracasei CNCM I-4034, Bifidobacterium breve CNCM I-4035 and Lactobacillus rhamnosus CNCM I-4036 to obese Zucker-Lepr fa/fa rats attenuates liver steatosis and exerts anti-inflammatory effects. The goal of the present work was to investigate the modulation of gene expression in intestinal mucosa samples of obese Zucker-Lepr fa/fa rats fed the probiotic strains using a DNA microarray and postgenomic techniques. We also measured secretory IgA content in the gut and lipopolysaccharide (LPS)-binding protein (LBP) in serum. Expression of three genes (Adamdec1, Ednrb and Ptgs1/Cox1) was up-regulated in the intestinal mucosa of the obese rats compared with that in the rats when they were still lean. Probiotic administration down-regulated expression of Adamdec1 and Ednrb at the mRNA and protein levels and that of Ptgs1/Cox1 at the mRNA level, and this effect was in part mediated by a decrease in both macrophage and dendritic cell populations. Probiotic treatment also increased secretory IgA content and diminished the LBP concentration. Based on results reported in this work and else where, we propose a possible mechanism of action for these bacterial strains.Julio Plaza-DíazCándido Robles-SánchezFrancisco Abadía-MolinaVirginia Morón-CalventeMaría José Sáez-LaraAlfonso Ruiz-BravoMaría Jiménez-ValeraÁngel GilCarolina Gómez-LlorenteLuis FontanaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Julio Plaza-Díaz Cándido Robles-Sánchez Francisco Abadía-Molina Virginia Morón-Calvente María José Sáez-Lara Alfonso Ruiz-Bravo María Jiménez-Valera Ángel Gil Carolina Gómez-Llorente Luis Fontana Adamdec1, Ednrb and Ptgs1/Cox1, inflammation genes upregulated in the intestinal mucosa of obese rats, are downregulated by three probiotic strains
description	Abstract We have previously reported that administration of Lactobacillus paracasei CNCM I-4034, Bifidobacterium breve CNCM I-4035 and Lactobacillus rhamnosus CNCM I-4036 to obese Zucker-Lepr fa/fa rats attenuates liver steatosis and exerts anti-inflammatory effects. The goal of the present work was to investigate the modulation of gene expression in intestinal mucosa samples of obese Zucker-Lepr fa/fa rats fed the probiotic strains using a DNA microarray and postgenomic techniques. We also measured secretory IgA content in the gut and lipopolysaccharide (LPS)-binding protein (LBP) in serum. Expression of three genes (Adamdec1, Ednrb and Ptgs1/Cox1) was up-regulated in the intestinal mucosa of the obese rats compared with that in the rats when they were still lean. Probiotic administration down-regulated expression of Adamdec1 and Ednrb at the mRNA and protein levels and that of Ptgs1/Cox1 at the mRNA level, and this effect was in part mediated by a decrease in both macrophage and dendritic cell populations. Probiotic treatment also increased secretory IgA content and diminished the LBP concentration. Based on results reported in this work and else where, we propose a possible mechanism of action for these bacterial strains.
format	article
author	Julio Plaza-Díaz Cándido Robles-Sánchez Francisco Abadía-Molina Virginia Morón-Calvente María José Sáez-Lara Alfonso Ruiz-Bravo María Jiménez-Valera Ángel Gil Carolina Gómez-Llorente Luis Fontana
author_facet	Julio Plaza-Díaz Cándido Robles-Sánchez Francisco Abadía-Molina Virginia Morón-Calvente María José Sáez-Lara Alfonso Ruiz-Bravo María Jiménez-Valera Ángel Gil Carolina Gómez-Llorente Luis Fontana
author_sort	Julio Plaza-Díaz
title	Adamdec1, Ednrb and Ptgs1/Cox1, inflammation genes upregulated in the intestinal mucosa of obese rats, are downregulated by three probiotic strains
title_short	Adamdec1, Ednrb and Ptgs1/Cox1, inflammation genes upregulated in the intestinal mucosa of obese rats, are downregulated by three probiotic strains
title_full	Adamdec1, Ednrb and Ptgs1/Cox1, inflammation genes upregulated in the intestinal mucosa of obese rats, are downregulated by three probiotic strains
title_fullStr	Adamdec1, Ednrb and Ptgs1/Cox1, inflammation genes upregulated in the intestinal mucosa of obese rats, are downregulated by three probiotic strains
title_full_unstemmed	Adamdec1, Ednrb and Ptgs1/Cox1, inflammation genes upregulated in the intestinal mucosa of obese rats, are downregulated by three probiotic strains
title_sort	adamdec1, ednrb and ptgs1/cox1, inflammation genes upregulated in the intestinal mucosa of obese rats, are downregulated by three probiotic strains
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/4897946dd49e4222819f7c9d6c31fdd4
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Adamdec1, Ednrb and Ptgs1/Cox1, inflammation genes upregulated in the intestinal mucosa of obese rats, are downregulated by three probiotic strains

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