<i>Zobellia barbeyronii</i> sp. nov., a New Member of the Family <i>Flavobacteriaceae</i>, Isolated from Seaweed, and Emended Description of the Species <i>Z. amurskyensis</i>, <i>Z. laminariae</i>, <i>Z. russellii</i> and <i>Z. uliginosa</i>

<i>Zobellia barbeyronii</i> sp. nov., a New Member of the Family <i>Flavobacteriaceae</i>, Isolated from Seaweed, and Emended Description of the Species <i>Z. amurskyensis</i>, <i>Z. laminariae</i>, <i>Z. russellii</i> and <i>Z. uliginosa</i>

Six Gram-stain-negative, aerobic, rod-shaped, and motile by gliding bacterial strains were isolated from Pacific green and red algae. Phylogenetic analysis based on 16S rRNA gene sequences placed the novel strains into the genus <i>Zobellia</i> as a distinct evolutionary lineage close to...

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Autores principales: Olga Nedashkovskaya, Nadezhda Otstavnykh, Natalia Zhukova, Konstantin Guzev, Viktoria Chausova, Liudmila Tekutyeva, Valery Mikhailov, Marina Isaeva
Formato: article
Lenguaje:EN
Publicado: MDPI AG 2021
Materias:
<i>Zobellia barbeyronii</i>
<i>Flavobacteriaceae</i>
marine bacteria
phylogeny
alga
genome
Biology (General)
QH301-705.5
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spelling oai:doaj.org-article:4897d74ab69d4cffb3ae561324adf3fb2021-11-25T17:22:20Z<i>Zobellia barbeyronii</i> sp. nov., a New Member of the Family <i>Flavobacteriaceae</i>, Isolated from Seaweed, and Emended Description of the Species <i>Z. amurskyensis</i>, <i>Z. laminariae</i>, <i>Z. russellii</i> and <i>Z. uliginosa</i>10.3390/d131105201424-2818https://doaj.org/article/4897d74ab69d4cffb3ae561324adf3fb2021-10-01T00:00:00Zhttps://www.mdpi.com/1424-2818/13/11/520https://doaj.org/toc/1424-2818Six Gram-stain-negative, aerobic, rod-shaped, and motile by gliding bacterial strains were isolated from Pacific green and red algae. Phylogenetic analysis based on 16S rRNA gene sequences placed the novel strains into the genus <i>Zobellia</i> as a distinct evolutionary lineage close to <i>Zobellia nedashkovskayae</i> Asnod2-B07-B<sup>T</sup> and <i>Zobellia laminariae</i> KMM 3676<sup>T</sup> sharing the highest similarity of 99.7% and 99.5%, respectively. The average nucleotide identity and the average amino acid identity values between strains 36-CHABK-3-33<sup>T</sup> and <i>Z. nedashkovskayae</i> Asnod2-B07-B<sup>T</sup> and <i>Z. laminariae</i> KMM 3676<sup>T</sup> were 89.7%/92.9% and 94.2%/95.8%, respectively. The digital DNA–DNA hybridization values based on the draft genomes between strains 36-CHABK-3-33<sup>T</sup> and <i>Z. nedashovskayae</i> Asnod2-B07-B<sup>T</sup> and <i>Z. laminariae</i> KMM 3676<sup>T</sup> were 39.5 ± 2.5% and 59.6 ± 2.7%, respectively. Multilocus sequence analysis based on house-keeping genes (<i>dnaK</i>, <i>gyrB</i>, <i>pyrH</i>, <i>recA</i> and <i>topA</i>) assigned the alga-associated isolates to the same species, which clustered separately from the recognized species of the genus <i>Zobellia</i>. The strains under study grew at 4–32 °C and with 0.5–8% NaCl and decomposed aesculin, gelatin, DNA, and Tweens 20 and 80, and weakly agar. The DNA G+C content was 36.7% calculated from genome sequence analysis for the strain 36-CHABK-3-33<sup>T</sup>. The predominant fatty acids of strain 36-CHABK-3-33<sup>T</sup> (>5% of the total fatty acids) were iso-C<sub>17:0</sub> 3-OH, summed feature 3 (comprising C<sub>16:1</sub><i>ω7c</i> and/or iso-C<sub>15:0</sub> 2-OH fatty acids), iso-C<sub>15:0</sub>, iso-C<sub>15:1</sub> G, and C<sub>15:0.</sub> The major polar lipids were phosphatidylethanolamine, three unidentified lipids, and two unidentified aminolipids. The only detected respiratory quinone was MK-6. The significant molecular distinctiveness between the novel isolates and their nearest neighbor was strongly supported by differences in physiological and biochemical tests. Therefore, the six novel strains represent a novel species of the genus <i>Zobellia</i>, for which the name <i>Zobellia</i> <i>barbeyronii</i> sp. nov. is proposed. The type strain is 36-CHABK-3-33<sup>T</sup> (= KACC 21790<sup>T</sup> = KMM 6746<sup>T</sup>).Olga NedashkovskayaNadezhda OtstavnykhNatalia ZhukovaKonstantin GuzevViktoria ChausovaLiudmila TekutyevaValery MikhailovMarina IsaevaMDPI AGarticle<i>Zobellia barbeyronii</i><i>Flavobacteriaceae</i>marine bacteriaphylogenyalgagenomeBiology (General)QH301-705.5ENDiversity, Vol 13, Iss 520, p 520 (2021)
institution DOAJ
collection DOAJ
language EN
topic <i>Zobellia barbeyronii</i>
<i>Flavobacteriaceae</i>
marine bacteria
phylogeny
alga
genome
Biology (General)
QH301-705.5
spellingShingle <i>Zobellia barbeyronii</i>
<i>Flavobacteriaceae</i>
marine bacteria
phylogeny
alga
genome
Biology (General)
QH301-705.5
Olga Nedashkovskaya
Nadezhda Otstavnykh
Natalia Zhukova
Konstantin Guzev
Viktoria Chausova
Liudmila Tekutyeva
Valery Mikhailov
Marina Isaeva
<i>Zobellia barbeyronii</i> sp. nov., a New Member of the Family <i>Flavobacteriaceae</i>, Isolated from Seaweed, and Emended Description of the Species <i>Z. amurskyensis</i>, <i>Z. laminariae</i>, <i>Z. russellii</i> and <i>Z. uliginosa</i>
description Six Gram-stain-negative, aerobic, rod-shaped, and motile by gliding bacterial strains were isolated from Pacific green and red algae. Phylogenetic analysis based on 16S rRNA gene sequences placed the novel strains into the genus <i>Zobellia</i> as a distinct evolutionary lineage close to <i>Zobellia nedashkovskayae</i> Asnod2-B07-B<sup>T</sup> and <i>Zobellia laminariae</i> KMM 3676<sup>T</sup> sharing the highest similarity of 99.7% and 99.5%, respectively. The average nucleotide identity and the average amino acid identity values between strains 36-CHABK-3-33<sup>T</sup> and <i>Z. nedashkovskayae</i> Asnod2-B07-B<sup>T</sup> and <i>Z. laminariae</i> KMM 3676<sup>T</sup> were 89.7%/92.9% and 94.2%/95.8%, respectively. The digital DNA–DNA hybridization values based on the draft genomes between strains 36-CHABK-3-33<sup>T</sup> and <i>Z. nedashovskayae</i> Asnod2-B07-B<sup>T</sup> and <i>Z. laminariae</i> KMM 3676<sup>T</sup> were 39.5 ± 2.5% and 59.6 ± 2.7%, respectively. Multilocus sequence analysis based on house-keeping genes (<i>dnaK</i>, <i>gyrB</i>, <i>pyrH</i>, <i>recA</i> and <i>topA</i>) assigned the alga-associated isolates to the same species, which clustered separately from the recognized species of the genus <i>Zobellia</i>. The strains under study grew at 4–32 °C and with 0.5–8% NaCl and decomposed aesculin, gelatin, DNA, and Tweens 20 and 80, and weakly agar. The DNA G+C content was 36.7% calculated from genome sequence analysis for the strain 36-CHABK-3-33<sup>T</sup>. The predominant fatty acids of strain 36-CHABK-3-33<sup>T</sup> (>5% of the total fatty acids) were iso-C<sub>17:0</sub> 3-OH, summed feature 3 (comprising C<sub>16:1</sub><i>ω7c</i> and/or iso-C<sub>15:0</sub> 2-OH fatty acids), iso-C<sub>15:0</sub>, iso-C<sub>15:1</sub> G, and C<sub>15:0.</sub> The major polar lipids were phosphatidylethanolamine, three unidentified lipids, and two unidentified aminolipids. The only detected respiratory quinone was MK-6. The significant molecular distinctiveness between the novel isolates and their nearest neighbor was strongly supported by differences in physiological and biochemical tests. Therefore, the six novel strains represent a novel species of the genus <i>Zobellia</i>, for which the name <i>Zobellia</i> <i>barbeyronii</i> sp. nov. is proposed. The type strain is 36-CHABK-3-33<sup>T</sup> (= KACC 21790<sup>T</sup> = KMM 6746<sup>T</sup>).
format article
author Olga Nedashkovskaya
Nadezhda Otstavnykh
Natalia Zhukova
Konstantin Guzev
Viktoria Chausova
Liudmila Tekutyeva
Valery Mikhailov
Marina Isaeva
author_facet Olga Nedashkovskaya
Nadezhda Otstavnykh
Natalia Zhukova
Konstantin Guzev
Viktoria Chausova
Liudmila Tekutyeva
Valery Mikhailov
Marina Isaeva
author_sort Olga Nedashkovskaya
title <i>Zobellia barbeyronii</i> sp. nov., a New Member of the Family <i>Flavobacteriaceae</i>, Isolated from Seaweed, and Emended Description of the Species <i>Z. amurskyensis</i>, <i>Z. laminariae</i>, <i>Z. russellii</i> and <i>Z. uliginosa</i>
title_short <i>Zobellia barbeyronii</i> sp. nov., a New Member of the Family <i>Flavobacteriaceae</i>, Isolated from Seaweed, and Emended Description of the Species <i>Z. amurskyensis</i>, <i>Z. laminariae</i>, <i>Z. russellii</i> and <i>Z. uliginosa</i>
title_full <i>Zobellia barbeyronii</i> sp. nov., a New Member of the Family <i>Flavobacteriaceae</i>, Isolated from Seaweed, and Emended Description of the Species <i>Z. amurskyensis</i>, <i>Z. laminariae</i>, <i>Z. russellii</i> and <i>Z. uliginosa</i>
title_fullStr <i>Zobellia barbeyronii</i> sp. nov., a New Member of the Family <i>Flavobacteriaceae</i>, Isolated from Seaweed, and Emended Description of the Species <i>Z. amurskyensis</i>, <i>Z. laminariae</i>, <i>Z. russellii</i> and <i>Z. uliginosa</i>
title_full_unstemmed <i>Zobellia barbeyronii</i> sp. nov., a New Member of the Family <i>Flavobacteriaceae</i>, Isolated from Seaweed, and Emended Description of the Species <i>Z. amurskyensis</i>, <i>Z. laminariae</i>, <i>Z. russellii</i> and <i>Z. uliginosa</i>
title_sort <i>zobellia barbeyronii</i> sp. nov., a new member of the family <i>flavobacteriaceae</i>, isolated from seaweed, and emended description of the species <i>z. amurskyensis</i>, <i>z. laminariae</i>, <i>z. russellii</i> and <i>z. uliginosa</i>
publisher MDPI AG
publishDate 2021
url https://doaj.org/article/4897d74ab69d4cffb3ae561324adf3fb
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