Role of Hydrogen Bonding in Green Fluorescent Protein-like Chromophore Emission

Abstract The fluorescence emission from green fluorescent protein (GFP) is known to be heavily influenced by hydrogen bonding between the core fluorophore and the surrounding side chains or water molecules. Yet how to utilize this feature for modulating the fluorescence of GFP chromophore or GFP-lik...

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Autores principales:	Li Yang, Shifeng Nian, Guozhen Zhang, Edward Sharman, Hui Miao, Xuepeng Zhang, Xiaofeng Chen, Yi Luo, Jun Jiang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/489fe93f48004da9a41d4fbd33b0d56f
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spelling	oai:doaj.org-article:489fe93f48004da9a41d4fbd33b0d56f2021-12-02T15:09:33ZRole of Hydrogen Bonding in Green Fluorescent Protein-like Chromophore Emission10.1038/s41598-019-47660-02045-2322https://doaj.org/article/489fe93f48004da9a41d4fbd33b0d56f2019-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-47660-0https://doaj.org/toc/2045-2322Abstract The fluorescence emission from green fluorescent protein (GFP) is known to be heavily influenced by hydrogen bonding between the core fluorophore and the surrounding side chains or water molecules. Yet how to utilize this feature for modulating the fluorescence of GFP chromophore or GFP-like fluorophore still remains elusive. Here we present theoretical calculations to predict how hydrogen bonding could influence the excited states of the GFP-like fluorophores. These studies provide both a new perspective for understanding the photophysical properties of GFP as well as a solid basis for the rational design of GFP-based fluorophores.Li YangShifeng NianGuozhen ZhangEdward SharmanHui MiaoXuepeng ZhangXiaofeng ChenYi LuoJun JiangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-7 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Li Yang Shifeng Nian Guozhen Zhang Edward Sharman Hui Miao Xuepeng Zhang Xiaofeng Chen Yi Luo Jun Jiang Role of Hydrogen Bonding in Green Fluorescent Protein-like Chromophore Emission
description	Abstract The fluorescence emission from green fluorescent protein (GFP) is known to be heavily influenced by hydrogen bonding between the core fluorophore and the surrounding side chains or water molecules. Yet how to utilize this feature for modulating the fluorescence of GFP chromophore or GFP-like fluorophore still remains elusive. Here we present theoretical calculations to predict how hydrogen bonding could influence the excited states of the GFP-like fluorophores. These studies provide both a new perspective for understanding the photophysical properties of GFP as well as a solid basis for the rational design of GFP-based fluorophores.
format	article
author	Li Yang Shifeng Nian Guozhen Zhang Edward Sharman Hui Miao Xuepeng Zhang Xiaofeng Chen Yi Luo Jun Jiang
author_facet	Li Yang Shifeng Nian Guozhen Zhang Edward Sharman Hui Miao Xuepeng Zhang Xiaofeng Chen Yi Luo Jun Jiang
author_sort	Li Yang
title	Role of Hydrogen Bonding in Green Fluorescent Protein-like Chromophore Emission
title_short	Role of Hydrogen Bonding in Green Fluorescent Protein-like Chromophore Emission
title_full	Role of Hydrogen Bonding in Green Fluorescent Protein-like Chromophore Emission
title_fullStr	Role of Hydrogen Bonding in Green Fluorescent Protein-like Chromophore Emission
title_full_unstemmed	Role of Hydrogen Bonding in Green Fluorescent Protein-like Chromophore Emission
title_sort	role of hydrogen bonding in green fluorescent protein-like chromophore emission
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/489fe93f48004da9a41d4fbd33b0d56f
work_keys_str_mv	AT liyang roleofhydrogenbondingingreenfluorescentproteinlikechromophoreemission AT shifengnian roleofhydrogenbondingingreenfluorescentproteinlikechromophoreemission AT guozhenzhang roleofhydrogenbondingingreenfluorescentproteinlikechromophoreemission AT edwardsharman roleofhydrogenbondingingreenfluorescentproteinlikechromophoreemission AT huimiao roleofhydrogenbondingingreenfluorescentproteinlikechromophoreemission AT xuepengzhang roleofhydrogenbondingingreenfluorescentproteinlikechromophoreemission AT xiaofengchen roleofhydrogenbondingingreenfluorescentproteinlikechromophoreemission AT yiluo roleofhydrogenbondingingreenfluorescentproteinlikechromophoreemission AT junjiang roleofhydrogenbondingingreenfluorescentproteinlikechromophoreemission
_version_	1718387811711713280

Role of Hydrogen Bonding in Green Fluorescent Protein-like Chromophore Emission

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