Size and lipid modification determine liposomal Indocyanine green performance for tumor imaging in a model of rectal cancer

Abstract Localization of rectal tumors is a challenge in minimally invasive surgery due to the lack of tactile sensation. We had developed liposomal indocyanine green (Lip-ICG) for localization of rectal tumor. In this study we evaluated the effects of liposome size and lipid PEGylation on imaging....

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Autores principales:	Shoshi Bar-David, Liraz Larush, Noam Goder, Asaf Aizic, Ehud Zigmond, Chen Varol, Joseph Klausner, Shlomo Magdassi, Eran Nizri
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/48a320a22ddf425a9ab5891f7ba58bce
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spelling	oai:doaj.org-article:48a320a22ddf425a9ab5891f7ba58bce2021-12-02T15:10:02ZSize and lipid modification determine liposomal Indocyanine green performance for tumor imaging in a model of rectal cancer10.1038/s41598-019-45038-w2045-2322https://doaj.org/article/48a320a22ddf425a9ab5891f7ba58bce2019-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-45038-whttps://doaj.org/toc/2045-2322Abstract Localization of rectal tumors is a challenge in minimally invasive surgery due to the lack of tactile sensation. We had developed liposomal indocyanine green (Lip-ICG) for localization of rectal tumor. In this study we evaluated the effects of liposome size and lipid PEGylation on imaging. We used an endoscopically-guided orthotopic experimental rectal cancer model in which tumor fluorescence was determined at different time points after intravenous (i.v.) administration of Lip-ICG and PEGylated liposomes (PEG-Lip-ICG). Signal intensity was measured by tumor-to-background ratio (TBR), or normalized TBR (compared to TBR of free ICG). Fluorescence microscopy of tumor tissue was performed to determine fluorescence localization within the tissue and blood vessels. Liposomes of 60 nm showed an increased TBR compared with free ICG at 12 hours after i.v. injection: normalized TBR (nTBR) = 3.11 vs. 1, respectively (p = 0.006). Larger liposomes (100 nm and 140 nm) had comparable signal to free ICG (nTBR = 0.98 ± 0.02 and 0.78 ± 0.08, respectively), even when additional time points were examined (0.5, 3 and 24 hours). PEG-Lip- ICG were more efficient than Lip-ICG (TBR = 4.2 ± 0.18 vs. 2.5 ± 0.12, p < 0.01) presumably because of reduced uptake by the reticulo-endothelial system. ICG was found outside the capillaries in tumor margins. We conclude that size and lipid modification impact imaging intensity.Shoshi Bar-DavidLiraz LarushNoam GoderAsaf AizicEhud ZigmondChen VarolJoseph KlausnerShlomo MagdassiEran NizriNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-8 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shoshi Bar-David Liraz Larush Noam Goder Asaf Aizic Ehud Zigmond Chen Varol Joseph Klausner Shlomo Magdassi Eran Nizri Size and lipid modification determine liposomal Indocyanine green performance for tumor imaging in a model of rectal cancer
description	Abstract Localization of rectal tumors is a challenge in minimally invasive surgery due to the lack of tactile sensation. We had developed liposomal indocyanine green (Lip-ICG) for localization of rectal tumor. In this study we evaluated the effects of liposome size and lipid PEGylation on imaging. We used an endoscopically-guided orthotopic experimental rectal cancer model in which tumor fluorescence was determined at different time points after intravenous (i.v.) administration of Lip-ICG and PEGylated liposomes (PEG-Lip-ICG). Signal intensity was measured by tumor-to-background ratio (TBR), or normalized TBR (compared to TBR of free ICG). Fluorescence microscopy of tumor tissue was performed to determine fluorescence localization within the tissue and blood vessels. Liposomes of 60 nm showed an increased TBR compared with free ICG at 12 hours after i.v. injection: normalized TBR (nTBR) = 3.11 vs. 1, respectively (p = 0.006). Larger liposomes (100 nm and 140 nm) had comparable signal to free ICG (nTBR = 0.98 ± 0.02 and 0.78 ± 0.08, respectively), even when additional time points were examined (0.5, 3 and 24 hours). PEG-Lip- ICG were more efficient than Lip-ICG (TBR = 4.2 ± 0.18 vs. 2.5 ± 0.12, p < 0.01) presumably because of reduced uptake by the reticulo-endothelial system. ICG was found outside the capillaries in tumor margins. We conclude that size and lipid modification impact imaging intensity.
format	article
author	Shoshi Bar-David Liraz Larush Noam Goder Asaf Aizic Ehud Zigmond Chen Varol Joseph Klausner Shlomo Magdassi Eran Nizri
author_facet	Shoshi Bar-David Liraz Larush Noam Goder Asaf Aizic Ehud Zigmond Chen Varol Joseph Klausner Shlomo Magdassi Eran Nizri
author_sort	Shoshi Bar-David
title	Size and lipid modification determine liposomal Indocyanine green performance for tumor imaging in a model of rectal cancer
title_short	Size and lipid modification determine liposomal Indocyanine green performance for tumor imaging in a model of rectal cancer
title_full	Size and lipid modification determine liposomal Indocyanine green performance for tumor imaging in a model of rectal cancer
title_fullStr	Size and lipid modification determine liposomal Indocyanine green performance for tumor imaging in a model of rectal cancer
title_full_unstemmed	Size and lipid modification determine liposomal Indocyanine green performance for tumor imaging in a model of rectal cancer
title_sort	size and lipid modification determine liposomal indocyanine green performance for tumor imaging in a model of rectal cancer
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/48a320a22ddf425a9ab5891f7ba58bce
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Size and lipid modification determine liposomal Indocyanine green performance for tumor imaging in a model of rectal cancer

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