Towards Celiac-safe foods: Decreasing the affinity of transglutaminase 2 for gliadin by addition of ascorbyl palmitate and ZnCl2 as detoxifiers

Abstract Initiation of celiac disease is triggered in the gastrointestinal tract by transglutaminase 2 (TG2) assisted deamidation of gluten peptides. Deamidation is a side-reaction to transamidation and occurs if primary amines are absent. In contrast to deamidation, transamidation does not trigger...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	N. Engstrom, P. Saenz-Méndez, J. Scheers, N. Scheers
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/48a46b15519c49e5a496323c30b4bbba
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:48a46b15519c49e5a496323c30b4bbba
record_format	dspace
spelling	oai:doaj.org-article:48a46b15519c49e5a496323c30b4bbba2021-12-02T15:04:55ZTowards Celiac-safe foods: Decreasing the affinity of transglutaminase 2 for gliadin by addition of ascorbyl palmitate and ZnCl2 as detoxifiers10.1038/s41598-017-00174-z2045-2322https://doaj.org/article/48a46b15519c49e5a496323c30b4bbba2017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00174-zhttps://doaj.org/toc/2045-2322Abstract Initiation of celiac disease is triggered in the gastrointestinal tract by transglutaminase 2 (TG2) assisted deamidation of gluten peptides. Deamidation is a side-reaction to transamidation and occurs if primary amines are absent. In contrast to deamidation, transamidation does not trigger an immune response. The aim of the study was to identify a suitable food additive that interacts with TG2 binding motives in gluten-derived peptides to prevent deamidation/transamidation. Homology modelling of α2-gliadin and computational screening of compounds for their binding affinity to a common TG2 binding motive (P)QLP were done by using computational approaches followed by experimental testing of TG2 activity. A database containing 1174 potential food grade ligands was screened against the model of α2-gliadin (27 out of 33 aa). Out of the five best ligands, ascorbyl palmitate, was observed to decrease TG2 transamidation of gliadin by 82% ± 2%. To completely silence the transamidation, we added zinc chloride (ZnCl2), and thereby reached a 99% ± 1% inhibition of TG2 activity. In addition, we conducted a pilot experiment in which ascorbyl palmitate was observed to decrease TG2 deamidation of gliadin completely. We propose ascorbyl palmitate in combination with ZnCl2 with the future perspective to become an additive in celiac-safe foods.N. EngstromP. Saenz-MéndezJ. ScheersN. ScheersNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q N. Engstrom P. Saenz-Méndez J. Scheers N. Scheers Towards Celiac-safe foods: Decreasing the affinity of transglutaminase 2 for gliadin by addition of ascorbyl palmitate and ZnCl2 as detoxifiers
description	Abstract Initiation of celiac disease is triggered in the gastrointestinal tract by transglutaminase 2 (TG2) assisted deamidation of gluten peptides. Deamidation is a side-reaction to transamidation and occurs if primary amines are absent. In contrast to deamidation, transamidation does not trigger an immune response. The aim of the study was to identify a suitable food additive that interacts with TG2 binding motives in gluten-derived peptides to prevent deamidation/transamidation. Homology modelling of α2-gliadin and computational screening of compounds for their binding affinity to a common TG2 binding motive (P)QLP were done by using computational approaches followed by experimental testing of TG2 activity. A database containing 1174 potential food grade ligands was screened against the model of α2-gliadin (27 out of 33 aa). Out of the five best ligands, ascorbyl palmitate, was observed to decrease TG2 transamidation of gliadin by 82% ± 2%. To completely silence the transamidation, we added zinc chloride (ZnCl2), and thereby reached a 99% ± 1% inhibition of TG2 activity. In addition, we conducted a pilot experiment in which ascorbyl palmitate was observed to decrease TG2 deamidation of gliadin completely. We propose ascorbyl palmitate in combination with ZnCl2 with the future perspective to become an additive in celiac-safe foods.
format	article
author	N. Engstrom P. Saenz-Méndez J. Scheers N. Scheers
author_facet	N. Engstrom P. Saenz-Méndez J. Scheers N. Scheers
author_sort	N. Engstrom
title	Towards Celiac-safe foods: Decreasing the affinity of transglutaminase 2 for gliadin by addition of ascorbyl palmitate and ZnCl2 as detoxifiers
title_short	Towards Celiac-safe foods: Decreasing the affinity of transglutaminase 2 for gliadin by addition of ascorbyl palmitate and ZnCl2 as detoxifiers
title_full	Towards Celiac-safe foods: Decreasing the affinity of transglutaminase 2 for gliadin by addition of ascorbyl palmitate and ZnCl2 as detoxifiers
title_fullStr	Towards Celiac-safe foods: Decreasing the affinity of transglutaminase 2 for gliadin by addition of ascorbyl palmitate and ZnCl2 as detoxifiers
title_full_unstemmed	Towards Celiac-safe foods: Decreasing the affinity of transglutaminase 2 for gliadin by addition of ascorbyl palmitate and ZnCl2 as detoxifiers
title_sort	towards celiac-safe foods: decreasing the affinity of transglutaminase 2 for gliadin by addition of ascorbyl palmitate and zncl2 as detoxifiers
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/48a46b15519c49e5a496323c30b4bbba
work_keys_str_mv	AT nengstrom towardsceliacsafefoodsdecreasingtheaffinityoftransglutaminase2forgliadinbyadditionofascorbylpalmitateandzncl2asdetoxifiers AT psaenzmendez towardsceliacsafefoodsdecreasingtheaffinityoftransglutaminase2forgliadinbyadditionofascorbylpalmitateandzncl2asdetoxifiers AT jscheers towardsceliacsafefoodsdecreasingtheaffinityoftransglutaminase2forgliadinbyadditionofascorbylpalmitateandzncl2asdetoxifiers AT nscheers towardsceliacsafefoodsdecreasingtheaffinityoftransglutaminase2forgliadinbyadditionofascorbylpalmitateandzncl2asdetoxifiers
_version_	1718388951787503616

Towards Celiac-safe foods: Decreasing the affinity of transglutaminase 2 for gliadin by addition of ascorbyl palmitate and ZnCl2 as detoxifiers

Ejemplares similares