Cell proliferation assay – method optimisation for in vivo labeling of DNA in the rat forestomach

The study of cell proliferation is a useful tool in the fields of toxicology, pathophysiology and pharmacology. Cell proliferation and its degree can be evaluated using 5-bromo-2′-deoxyuridine which is incorporated into the newly synthesized DNA. The aim of this study was the optimization of subcuta...

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Autores principales:	Joksić Gordana, Mićić Mileva, Filipović Jelena, Drakulić Dunja, Stanojlović Miloš, Čalija Bojan, Valenta Šobot Ana, Demajo Miroslav, Nilsson Robert
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2017
Materias:	5-bromo-2′-deoxyuridine 3-tert-butyl-4-hydroxyanisole brdu in vivo labeling cell proliferation rat forestomach Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/48a56385f97c47fb9829117a46c0aa98
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spelling	oai:doaj.org-article:48a56385f97c47fb9829117a46c0aa982021-11-17T21:27:51ZCell proliferation assay – method optimisation for in vivo labeling of DNA in the rat forestomach1820-744810.1515/acve-2017-0001https://doaj.org/article/48a56385f97c47fb9829117a46c0aa982017-03-01T00:00:00Zhttps://doi.org/10.1515/acve-2017-0001https://doaj.org/toc/1820-7448The study of cell proliferation is a useful tool in the fields of toxicology, pathophysiology and pharmacology. Cell proliferation and its degree can be evaluated using 5-bromo-2′-deoxyuridine which is incorporated into the newly synthesized DNA. The aim of this study was the optimization of subcutaneous application of 5-bromo-2′-deoxyuridine implantation for continuous and persistent marking of proliferating cells in the rat forestomach. 3-tert-Butyl-4-hydroxyanisole was used as the agent that ensures cell proliferation. In order to determine the optimal dose for proliferating cells labeling, 5-bromo-2′-deoxyuridine doses of 50 mg, 100 mg, 200 mg or 350 mg were implemented 2 days prior to sacrifice by flat-faced cylindrical matrices. Immunohistochemical analysis using 5-bromo-2′-deoxyuridine in situ detection kit was performed for the detection of 5-bromo-2′-deoxyuridine labeled cells. The results showed that for adult rats, the optimum 5-bromo-2′-deoxyuridine dose is 200 mg per animal for subcutaneous application. The here described manner of 5-bromo-2′-deoxyuridine in vivo labeling provides a simple, efficient, and reliable method for cell labeling, and at the same minimizes stress to animals.Joksić GordanaMićić MilevaFilipović JelenaDrakulić DunjaStanojlović MilošČalija BojanValenta Šobot AnaDemajo MiroslavNilsson RobertSciendoarticle5-bromo-2′-deoxyuridine3-tert-butyl-4-hydroxyanisolebrdu in vivo labelingcell proliferationrat forestomachVeterinary medicineSF600-1100ENActa Veterinaria, Vol 67, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	5-bromo-2′-deoxyuridine 3-tert-butyl-4-hydroxyanisole brdu in vivo labeling cell proliferation rat forestomach Veterinary medicine SF600-1100
spellingShingle	5-bromo-2′-deoxyuridine 3-tert-butyl-4-hydroxyanisole brdu in vivo labeling cell proliferation rat forestomach Veterinary medicine SF600-1100 Joksić Gordana Mićić Mileva Filipović Jelena Drakulić Dunja Stanojlović Miloš Čalija Bojan Valenta Šobot Ana Demajo Miroslav Nilsson Robert Cell proliferation assay – method optimisation for in vivo labeling of DNA in the rat forestomach
description	The study of cell proliferation is a useful tool in the fields of toxicology, pathophysiology and pharmacology. Cell proliferation and its degree can be evaluated using 5-bromo-2′-deoxyuridine which is incorporated into the newly synthesized DNA. The aim of this study was the optimization of subcutaneous application of 5-bromo-2′-deoxyuridine implantation for continuous and persistent marking of proliferating cells in the rat forestomach. 3-tert-Butyl-4-hydroxyanisole was used as the agent that ensures cell proliferation. In order to determine the optimal dose for proliferating cells labeling, 5-bromo-2′-deoxyuridine doses of 50 mg, 100 mg, 200 mg or 350 mg were implemented 2 days prior to sacrifice by flat-faced cylindrical matrices. Immunohistochemical analysis using 5-bromo-2′-deoxyuridine in situ detection kit was performed for the detection of 5-bromo-2′-deoxyuridine labeled cells. The results showed that for adult rats, the optimum 5-bromo-2′-deoxyuridine dose is 200 mg per animal for subcutaneous application. The here described manner of 5-bromo-2′-deoxyuridine in vivo labeling provides a simple, efficient, and reliable method for cell labeling, and at the same minimizes stress to animals.
format	article
author	Joksić Gordana Mićić Mileva Filipović Jelena Drakulić Dunja Stanojlović Miloš Čalija Bojan Valenta Šobot Ana Demajo Miroslav Nilsson Robert
author_facet	Joksić Gordana Mićić Mileva Filipović Jelena Drakulić Dunja Stanojlović Miloš Čalija Bojan Valenta Šobot Ana Demajo Miroslav Nilsson Robert
author_sort	Joksić Gordana
title	Cell proliferation assay – method optimisation for in vivo labeling of DNA in the rat forestomach
title_short	Cell proliferation assay – method optimisation for in vivo labeling of DNA in the rat forestomach
title_full	Cell proliferation assay – method optimisation for in vivo labeling of DNA in the rat forestomach
title_fullStr	Cell proliferation assay – method optimisation for in vivo labeling of DNA in the rat forestomach
title_full_unstemmed	Cell proliferation assay – method optimisation for in vivo labeling of DNA in the rat forestomach
title_sort	cell proliferation assay – method optimisation for in vivo labeling of dna in the rat forestomach
publisher	Sciendo
publishDate	2017
url	https://doaj.org/article/48a56385f97c47fb9829117a46c0aa98
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Cell proliferation assay – method optimisation for in vivo labeling of DNA in the rat forestomach

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