Scavenger receptors in human airway epithelial cells: role in response to double-stranded RNA.

Scavenger receptors and Toll-like receptors (TLRs) cooperate in response to danger signals to adjust the host immune response. The TLR3 agonist double stranded (ds)RNA is an efficient activator of innate signalling in bronchial epithelial cells. In this study, we aimed at defining the role played by...

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Autores principales:	Audrey Dieudonné, David Torres, Simon Blanchard, Solenne Taront, Pascale Jeannin, Yves Delneste, Muriel Pichavant, François Trottein, Philippe Gosset
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/48ae00b21d164f8f918dbb771e2cbaca
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spelling	oai:doaj.org-article:48ae00b21d164f8f918dbb771e2cbaca2021-11-18T07:09:28ZScavenger receptors in human airway epithelial cells: role in response to double-stranded RNA.1932-620310.1371/journal.pone.0041952https://doaj.org/article/48ae00b21d164f8f918dbb771e2cbaca2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22879901/?tool=EBIhttps://doaj.org/toc/1932-6203Scavenger receptors and Toll-like receptors (TLRs) cooperate in response to danger signals to adjust the host immune response. The TLR3 agonist double stranded (ds)RNA is an efficient activator of innate signalling in bronchial epithelial cells. In this study, we aimed at defining the role played by scavenger receptors expressed by bronchial epithelial cells in the control of the innate response to dsRNA both in vitro and in vivo. Expression of several scavenger receptor involved in pathogen recognition was first evaluated in human bronchial epithelial cells in steady-state and inflammatory conditions. Their implication in the uptake of dsRNA and the subsequent cell activation was evaluated in vitro by competition with ligand of scavenger receptors including maleylated ovalbumin and by RNA silencing. The capacity of maleylated ovalbumin to modulate lung inflammation induced by dsRNA was also investigated in mice. Exposure to tumor necrosis factor-α increased expression of the scavenger receptors LOX-1 and CXCL16 and the capacity to internalize maleylated ovalbumin, whereas activation by TLR ligands did not. In contrast, the expression of SR-B1 was not modulated in these conditions. Interestingly, supplementation with maleylated ovalbumin limited dsRNA uptake and inhibited subsequent activation of bronchial epithelial cells. RNA silencing of LOX-1 and SR-B1 strongly blocked the dsRNA-induced cytokine production. Finally, administration of maleylated ovalbumin in mice inhibited the dsRNA-induced infiltration and activation of inflammatory cells in bronchoalveolar spaces and lung draining lymph nodes. Together, our data characterize the function of SR-B1 and LOX-1 in bronchial epithelial cells and their implication in dsRNA-induced responses, a finding that might be relevant during respiratory viral infections.Audrey DieudonnéDavid TorresSimon BlanchardSolenne TarontPascale JeanninYves DelnesteMuriel PichavantFrançois TrotteinPhilippe GossetPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 8, p e41952 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Audrey Dieudonné David Torres Simon Blanchard Solenne Taront Pascale Jeannin Yves Delneste Muriel Pichavant François Trottein Philippe Gosset Scavenger receptors in human airway epithelial cells: role in response to double-stranded RNA.
description	Scavenger receptors and Toll-like receptors (TLRs) cooperate in response to danger signals to adjust the host immune response. The TLR3 agonist double stranded (ds)RNA is an efficient activator of innate signalling in bronchial epithelial cells. In this study, we aimed at defining the role played by scavenger receptors expressed by bronchial epithelial cells in the control of the innate response to dsRNA both in vitro and in vivo. Expression of several scavenger receptor involved in pathogen recognition was first evaluated in human bronchial epithelial cells in steady-state and inflammatory conditions. Their implication in the uptake of dsRNA and the subsequent cell activation was evaluated in vitro by competition with ligand of scavenger receptors including maleylated ovalbumin and by RNA silencing. The capacity of maleylated ovalbumin to modulate lung inflammation induced by dsRNA was also investigated in mice. Exposure to tumor necrosis factor-α increased expression of the scavenger receptors LOX-1 and CXCL16 and the capacity to internalize maleylated ovalbumin, whereas activation by TLR ligands did not. In contrast, the expression of SR-B1 was not modulated in these conditions. Interestingly, supplementation with maleylated ovalbumin limited dsRNA uptake and inhibited subsequent activation of bronchial epithelial cells. RNA silencing of LOX-1 and SR-B1 strongly blocked the dsRNA-induced cytokine production. Finally, administration of maleylated ovalbumin in mice inhibited the dsRNA-induced infiltration and activation of inflammatory cells in bronchoalveolar spaces and lung draining lymph nodes. Together, our data characterize the function of SR-B1 and LOX-1 in bronchial epithelial cells and their implication in dsRNA-induced responses, a finding that might be relevant during respiratory viral infections.
format	article
author	Audrey Dieudonné David Torres Simon Blanchard Solenne Taront Pascale Jeannin Yves Delneste Muriel Pichavant François Trottein Philippe Gosset
author_facet	Audrey Dieudonné David Torres Simon Blanchard Solenne Taront Pascale Jeannin Yves Delneste Muriel Pichavant François Trottein Philippe Gosset
author_sort	Audrey Dieudonné
title	Scavenger receptors in human airway epithelial cells: role in response to double-stranded RNA.
title_short	Scavenger receptors in human airway epithelial cells: role in response to double-stranded RNA.
title_full	Scavenger receptors in human airway epithelial cells: role in response to double-stranded RNA.
title_fullStr	Scavenger receptors in human airway epithelial cells: role in response to double-stranded RNA.
title_full_unstemmed	Scavenger receptors in human airway epithelial cells: role in response to double-stranded RNA.
title_sort	scavenger receptors in human airway epithelial cells: role in response to double-stranded rna.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/48ae00b21d164f8f918dbb771e2cbaca
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Scavenger receptors in human airway epithelial cells: role in response to double-stranded RNA.

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