An integrated approach for identification of a panel of candidate genes arbitrated for invasion and metastasis in oral squamous cell carcinoma

Abstract Oral squamous cell carcinoma (OSCC) is known for its aggressiveness associated with poor prognosis. The molecular mechanisms underlying the invasion and metastasis are still poorly understood. An improved understanding of these mechanisms shall precede the development of new diagnostic tool...

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Autores principales:	Samapika Routray, Ravindra Kumar, Keshava K. Datta, Vinuth N. Puttamallesh, Aditi Chatterjee, Harsha Gowda, Neeta Mohanty, Rupesh Dash, Anshuman Dixit
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/48b0e56ccfac4eb0bf1df1d7a58d021d
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spelling	oai:doaj.org-article:48b0e56ccfac4eb0bf1df1d7a58d021d2021-12-02T13:17:55ZAn integrated approach for identification of a panel of candidate genes arbitrated for invasion and metastasis in oral squamous cell carcinoma10.1038/s41598-021-85729-x2045-2322https://doaj.org/article/48b0e56ccfac4eb0bf1df1d7a58d021d2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85729-xhttps://doaj.org/toc/2045-2322Abstract Oral squamous cell carcinoma (OSCC) is known for its aggressiveness associated with poor prognosis. The molecular mechanisms underlying the invasion and metastasis are still poorly understood. An improved understanding of these mechanisms shall precede the development of new diagnostic tools and targeted therapies. We report an integrated approach using bioinformatics to predict candidate genes, coupled with proteomics and immunohistochemistry for validating their presence and involvement in OSCC pathways heralding invasion and metastasis. Four genes POSTN, TNC, CAV1 and FSCN1 were identified. A protein–protein interaction network analysis teamed with pathway analysis led us to propose the role of the identified genes in invasion and metastasis in OSCC. Further analyses of archived FFPE blocks of various grades of oral cancer was carried out using TMT-based mass spectrometry and immunohistochemistry. Results of this study expressed a strong communiqué and interrelationship between these candidate genes. This study emphasizes the significance of a molecular biomarker panel as a diagnostic tool and its correlation with the invasion and metastatic pathway of OSCC. An insight into the probable association of CAF's and these biomarkers in the evolution and malignant transformation of OSCC further magnifies the molecular-biological spectrum of OSCC tumour microenvironment.Samapika RoutrayRavindra KumarKeshava K. DattaVinuth N. PuttamalleshAditi ChatterjeeHarsha GowdaNeeta MohantyRupesh DashAnshuman DixitNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Samapika Routray Ravindra Kumar Keshava K. Datta Vinuth N. Puttamallesh Aditi Chatterjee Harsha Gowda Neeta Mohanty Rupesh Dash Anshuman Dixit An integrated approach for identification of a panel of candidate genes arbitrated for invasion and metastasis in oral squamous cell carcinoma
description	Abstract Oral squamous cell carcinoma (OSCC) is known for its aggressiveness associated with poor prognosis. The molecular mechanisms underlying the invasion and metastasis are still poorly understood. An improved understanding of these mechanisms shall precede the development of new diagnostic tools and targeted therapies. We report an integrated approach using bioinformatics to predict candidate genes, coupled with proteomics and immunohistochemistry for validating their presence and involvement in OSCC pathways heralding invasion and metastasis. Four genes POSTN, TNC, CAV1 and FSCN1 were identified. A protein–protein interaction network analysis teamed with pathway analysis led us to propose the role of the identified genes in invasion and metastasis in OSCC. Further analyses of archived FFPE blocks of various grades of oral cancer was carried out using TMT-based mass spectrometry and immunohistochemistry. Results of this study expressed a strong communiqué and interrelationship between these candidate genes. This study emphasizes the significance of a molecular biomarker panel as a diagnostic tool and its correlation with the invasion and metastatic pathway of OSCC. An insight into the probable association of CAF's and these biomarkers in the evolution and malignant transformation of OSCC further magnifies the molecular-biological spectrum of OSCC tumour microenvironment.
format	article
author	Samapika Routray Ravindra Kumar Keshava K. Datta Vinuth N. Puttamallesh Aditi Chatterjee Harsha Gowda Neeta Mohanty Rupesh Dash Anshuman Dixit
author_facet	Samapika Routray Ravindra Kumar Keshava K. Datta Vinuth N. Puttamallesh Aditi Chatterjee Harsha Gowda Neeta Mohanty Rupesh Dash Anshuman Dixit
author_sort	Samapika Routray
title	An integrated approach for identification of a panel of candidate genes arbitrated for invasion and metastasis in oral squamous cell carcinoma
title_short	An integrated approach for identification of a panel of candidate genes arbitrated for invasion and metastasis in oral squamous cell carcinoma
title_full	An integrated approach for identification of a panel of candidate genes arbitrated for invasion and metastasis in oral squamous cell carcinoma
title_fullStr	An integrated approach for identification of a panel of candidate genes arbitrated for invasion and metastasis in oral squamous cell carcinoma
title_full_unstemmed	An integrated approach for identification of a panel of candidate genes arbitrated for invasion and metastasis in oral squamous cell carcinoma
title_sort	integrated approach for identification of a panel of candidate genes arbitrated for invasion and metastasis in oral squamous cell carcinoma
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/48b0e56ccfac4eb0bf1df1d7a58d021d
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An integrated approach for identification of a panel of candidate genes arbitrated for invasion and metastasis in oral squamous cell carcinoma

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