Molecular Modeling the Proteins from the <i>exo-xis</i> Region of Lambda and Shigatoxigenic Bacteriophages
Despite decades of intensive research on bacteriophage lambda, a relatively uncharacterized region remains between the <i>exo</i> and <i>xis</i> genes. Collectively, <i>exo-xis</i> region genes are expressed during the earliest stages of the lytic developmental cy...
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| Formato: | article |
| Lenguaje: | EN |
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MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/48b2590acda844dd8f2f0e3be3f01cca |
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| Sumario: | Despite decades of intensive research on bacteriophage lambda, a relatively uncharacterized region remains between the <i>exo</i> and <i>xis</i> genes. Collectively, <i>exo-xis</i> region genes are expressed during the earliest stages of the lytic developmental cycle and are capable of affecting the molecular events associated with the lysogenic-lytic developmental decision. In Shiga toxin-producing <i>E. coli</i> (STEC) and enterohemorragic <i>E. coli</i> (EHEC) that are responsible for food- and water-borne outbreaks throughout the world, there are distinct differences of <i>exo-xis</i> region genes from their counterparts in lambda phage. Together, these differences may help EHEC-specific phage and their bacterial hosts adapt to the complex environment within the human intestine. Only one <i>exo-xis</i> region protein, Ea8.5, has been solved to date. Here, I have used the AlphaFold and RoseTTAFold machine learning algorithms to predict the structures of six <i>exo-xis</i> region proteins from lambda and STEC/EHEC phages. Together, the models suggest possible roles for <i>exo-xis</i> region proteins in transcription and the regulation of RNA polymerase. |
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