Study of Variability in Root System Architecture of Spanish Triticum turgidum L. Subspecies and Analysis of the Presence of a MITE Element Inserted in the TtDro1B Gene: Evolutionary Implications

We analysed nine traits of the root system of 223 genotypes of Triticum turgidum (2n = 4x = AABB) subspecies dicoccoides, dicoccum, turgidum, durum and polonicum, finding a large intra a...

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Autores principales:	Juan M. González, Rodrigo Cañas, Alejandra Cabeza, Magdalena Ruiz, Patricia Giraldo, Yolanda Loarce
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>durum</i> wheat germplasm plant genetic resources RSA transposon Agriculture S
Acceso en línea:	https://doaj.org/article/48bfd1f026cd48a2b2e364823338536f
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spelling	oai:doaj.org-article:48bfd1f026cd48a2b2e364823338536f2021-11-25T16:10:38ZStudy of Variability in Root System Architecture of Spanish <i>Triticum turgidum</i> L. Subspecies and Analysis of the Presence of a MITE Element Inserted in the <i>TtDro1B</i> Gene: Evolutionary Implications10.3390/agronomy111122942073-4395https://doaj.org/article/48bfd1f026cd48a2b2e364823338536f2021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4395/11/11/2294https://doaj.org/toc/2073-4395We analysed nine traits of the root system of 223 genotypes of <i>Triticum turgidum</i> (2n = 4x = AABB) subspecies <i>dicoccoides</i>, <i>dicoccum</i>, <i>turgidum</i>, <i>durum</i> and <i>polonicum</i>, finding a large intra and interspecific variability in both the number and size of roots, as well as in their spatial distribution. We studied the presence of an incomplete MITE (Miniature Inverted-repeat Transposable Element) inserted in the <i>TtDro1B</i> gene, which is present in some genotypes of <i>dicoccoides</i>, <i>dicoccum</i>, and <i>turgidum</i>, but not in <i>polonicum</i> and the 97.9% of the <i>durum</i> accessions. Comparison between genotypes shows that genotypes with the MITE element have smaller and shallower roots. Since <i>Aegilops</i> is considered to be the donor of the wheat B genome, the presence of the same MITE element was analysed in 55 accessions of the species <i>Aegilops speltoides</i>, <i>searsii</i>, <i>bicornis</i> and <i>longissima,</i> and in no case was it detected. We propose that after the emergence of <i>T. turgidum</i> subsp. <i>dicoccoides</i>, the insertion of the MITE element probably occurred in a single plant. Subsequent domestication resulted in genotypes of <i>dicoccum</i> with and without the MITE element, which after selection gave rise to the subspecies <i>turgidum</i>, and <i>durum</i> and <i>polonicum,</i> respectively. The MITE element can be used to differentiate <i>turgidum</i> from the <i>durum</i> and <i>polonicum</i> with high reliability.Juan M. GonzálezRodrigo CañasAlejandra CabezaMagdalena RuizPatricia GiraldoYolanda LoarceMDPI AGarticle<i>durum</i> wheatgermplasmplant genetic resourcesRSAtransposonAgricultureSENAgronomy, Vol 11, Iss 2294, p 2294 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>durum</i> wheat germplasm plant genetic resources RSA transposon Agriculture S
spellingShingle	<i>durum</i> wheat germplasm plant genetic resources RSA transposon Agriculture S Juan M. González Rodrigo Cañas Alejandra Cabeza Magdalena Ruiz Patricia Giraldo Yolanda Loarce Study of Variability in Root System Architecture of Spanish <i>Triticum turgidum</i> L. Subspecies and Analysis of the Presence of a MITE Element Inserted in the <i>TtDro1B</i> Gene: Evolutionary Implications
description	We analysed nine traits of the root system of 223 genotypes of <i>Triticum turgidum</i> (2n = 4x = AABB) subspecies <i>dicoccoides</i>, <i>dicoccum</i>, <i>turgidum</i>, <i>durum</i> and <i>polonicum</i>, finding a large intra and interspecific variability in both the number and size of roots, as well as in their spatial distribution. We studied the presence of an incomplete MITE (Miniature Inverted-repeat Transposable Element) inserted in the <i>TtDro1B</i> gene, which is present in some genotypes of <i>dicoccoides</i>, <i>dicoccum</i>, and <i>turgidum</i>, but not in <i>polonicum</i> and the 97.9% of the <i>durum</i> accessions. Comparison between genotypes shows that genotypes with the MITE element have smaller and shallower roots. Since <i>Aegilops</i> is considered to be the donor of the wheat B genome, the presence of the same MITE element was analysed in 55 accessions of the species <i>Aegilops speltoides</i>, <i>searsii</i>, <i>bicornis</i> and <i>longissima,</i> and in no case was it detected. We propose that after the emergence of <i>T. turgidum</i> subsp. <i>dicoccoides</i>, the insertion of the MITE element probably occurred in a single plant. Subsequent domestication resulted in genotypes of <i>dicoccum</i> with and without the MITE element, which after selection gave rise to the subspecies <i>turgidum</i>, and <i>durum</i> and <i>polonicum,</i> respectively. The MITE element can be used to differentiate <i>turgidum</i> from the <i>durum</i> and <i>polonicum</i> with high reliability.
format	article
author	Juan M. González Rodrigo Cañas Alejandra Cabeza Magdalena Ruiz Patricia Giraldo Yolanda Loarce
author_facet	Juan M. González Rodrigo Cañas Alejandra Cabeza Magdalena Ruiz Patricia Giraldo Yolanda Loarce
author_sort	Juan M. González
title	Study of Variability in Root System Architecture of Spanish <i>Triticum turgidum</i> L. Subspecies and Analysis of the Presence of a MITE Element Inserted in the <i>TtDro1B</i> Gene: Evolutionary Implications
title_short	Study of Variability in Root System Architecture of Spanish <i>Triticum turgidum</i> L. Subspecies and Analysis of the Presence of a MITE Element Inserted in the <i>TtDro1B</i> Gene: Evolutionary Implications
title_full	Study of Variability in Root System Architecture of Spanish <i>Triticum turgidum</i> L. Subspecies and Analysis of the Presence of a MITE Element Inserted in the <i>TtDro1B</i> Gene: Evolutionary Implications
title_fullStr	Study of Variability in Root System Architecture of Spanish <i>Triticum turgidum</i> L. Subspecies and Analysis of the Presence of a MITE Element Inserted in the <i>TtDro1B</i> Gene: Evolutionary Implications
title_full_unstemmed	Study of Variability in Root System Architecture of Spanish <i>Triticum turgidum</i> L. Subspecies and Analysis of the Presence of a MITE Element Inserted in the <i>TtDro1B</i> Gene: Evolutionary Implications
title_sort	study of variability in root system architecture of spanish <i>triticum turgidum</i> l. subspecies and analysis of the presence of a mite element inserted in the <i>ttdro1b</i> gene: evolutionary implications
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/48bfd1f026cd48a2b2e364823338536f
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Study of Variability in Root System Architecture of Spanish <i>Triticum turgidum</i> L. Subspecies and Analysis of the Presence of a MITE Element Inserted in the <i>TtDro1B</i> Gene: Evolutionary Implications

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