Poly((D,L)lactic-glycolic)acid–star glucose nanoparticles for glucose transporter and hypoglycemia-mediated tumor targeting
Ju-Hwan Park,1 Hyun-Jong Cho,2 Dae-Duk Kim1 1College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul, 2College of Pharmacy, Kangwon National University, Chuncheon, Gangwon, Republic of Korea Abstract: Poly((D,L)lactic-glycolic)acid–star glu...
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Dove Medical Press
2017
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oai:doaj.org-article:48c0a443f1d444128593fc1263ffb3f52021-12-02T04:23:13ZPoly((D,L)lactic-glycolic)acid–star glucose nanoparticles for glucose transporter and hypoglycemia-mediated tumor targeting1178-2013https://doaj.org/article/48c0a443f1d444128593fc1263ffb3f52017-10-01T00:00:00Zhttps://www.dovepress.com/polydllactic-glycolicacid-star-glucose-nanoparticles-for-glucose-trans-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Ju-Hwan Park,1 Hyun-Jong Cho,2 Dae-Duk Kim1 1College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul, 2College of Pharmacy, Kangwon National University, Chuncheon, Gangwon, Republic of Korea Abstract: Poly((D,L)lactic-glycolic)acid–star glucose (PLGA-Glc) polymer-based nanoparticles (NPs) were fabricated for tumor-targeted delivery of docetaxel (DCT). NPs with an approximate mean diameter of 241 nm, narrow size distribution, negative zeta potential, and spherical shape were prepared. A sustained drug release pattern from the developed NPs was observed for 13 days. Moreover, drug release from PLGA-Glc NPs at acidic pH (endocytic compartments and tumor regions) was significantly improved compared with that observed at physiological pH (normal tissues and organs). DCT-loaded PLGA-Glc NPs (DCT/PLGA-Glc NPs) exhibited an enhanced antiproliferation efficiency rather than DCT-loaded PLGA NPs (DCT/PLGA NPs) in Hep-2 cells, which can be regarded as glucose transporters (GLUTs)-positive cells, at ≥50 ng/mL DCT concentration range. Under glucose-deprived (hypoglycemic) conditions, the cellular uptake efficiency of the PLGA-Glc NPs was higher in Hep-2 cells compared to that observed in PLGA NPs. Cy5.5-loaded NPs were prepared and injected into a Hep-2 tumor-xenografted mouse model for in vivo near-infrared fluorescence imaging. The PLGA-Glc NPs group exhibited higher fluorescence intensity in the tumor region than the PLGA NPs group. These results imply that the PLGA-Glc NPs have active tumor targeting abilities based on interactions with GLUTs and the hypoglycemic conditions in the tumor region. Therefore, the developed PLGA-Glc NPs may represent a promising tumor-targeted delivery system for anticancer drugs. Keywords: PLGA-Glc, nanoparticles, glucose transporter, hypoxia, tumor targetingPark JHCho HJKim DDDove Medical PressarticlePLGA-Glcnanoparticlesglucose transporterhypoxiatumor targetingMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 7453-7467 (2017) |
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PLGA-Glc nanoparticles glucose transporter hypoxia tumor targeting Medicine (General) R5-920 |
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PLGA-Glc nanoparticles glucose transporter hypoxia tumor targeting Medicine (General) R5-920 Park JH Cho HJ Kim DD Poly((D,L)lactic-glycolic)acid–star glucose nanoparticles for glucose transporter and hypoglycemia-mediated tumor targeting |
| description |
Ju-Hwan Park,1 Hyun-Jong Cho,2 Dae-Duk Kim1 1College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul, 2College of Pharmacy, Kangwon National University, Chuncheon, Gangwon, Republic of Korea Abstract: Poly((D,L)lactic-glycolic)acid–star glucose (PLGA-Glc) polymer-based nanoparticles (NPs) were fabricated for tumor-targeted delivery of docetaxel (DCT). NPs with an approximate mean diameter of 241 nm, narrow size distribution, negative zeta potential, and spherical shape were prepared. A sustained drug release pattern from the developed NPs was observed for 13 days. Moreover, drug release from PLGA-Glc NPs at acidic pH (endocytic compartments and tumor regions) was significantly improved compared with that observed at physiological pH (normal tissues and organs). DCT-loaded PLGA-Glc NPs (DCT/PLGA-Glc NPs) exhibited an enhanced antiproliferation efficiency rather than DCT-loaded PLGA NPs (DCT/PLGA NPs) in Hep-2 cells, which can be regarded as glucose transporters (GLUTs)-positive cells, at ≥50 ng/mL DCT concentration range. Under glucose-deprived (hypoglycemic) conditions, the cellular uptake efficiency of the PLGA-Glc NPs was higher in Hep-2 cells compared to that observed in PLGA NPs. Cy5.5-loaded NPs were prepared and injected into a Hep-2 tumor-xenografted mouse model for in vivo near-infrared fluorescence imaging. The PLGA-Glc NPs group exhibited higher fluorescence intensity in the tumor region than the PLGA NPs group. These results imply that the PLGA-Glc NPs have active tumor targeting abilities based on interactions with GLUTs and the hypoglycemic conditions in the tumor region. Therefore, the developed PLGA-Glc NPs may represent a promising tumor-targeted delivery system for anticancer drugs. Keywords: PLGA-Glc, nanoparticles, glucose transporter, hypoxia, tumor targeting |
| format |
article |
| author |
Park JH Cho HJ Kim DD |
| author_facet |
Park JH Cho HJ Kim DD |
| author_sort |
Park JH |
| title |
Poly((D,L)lactic-glycolic)acid–star glucose nanoparticles for glucose transporter and hypoglycemia-mediated tumor targeting |
| title_short |
Poly((D,L)lactic-glycolic)acid–star glucose nanoparticles for glucose transporter and hypoglycemia-mediated tumor targeting |
| title_full |
Poly((D,L)lactic-glycolic)acid–star glucose nanoparticles for glucose transporter and hypoglycemia-mediated tumor targeting |
| title_fullStr |
Poly((D,L)lactic-glycolic)acid–star glucose nanoparticles for glucose transporter and hypoglycemia-mediated tumor targeting |
| title_full_unstemmed |
Poly((D,L)lactic-glycolic)acid–star glucose nanoparticles for glucose transporter and hypoglycemia-mediated tumor targeting |
| title_sort |
poly((d,l)lactic-glycolic)acid–star glucose nanoparticles for glucose transporter and hypoglycemia-mediated tumor targeting |
| publisher |
Dove Medical Press |
| publishDate |
2017 |
| url |
https://doaj.org/article/48c0a443f1d444128593fc1263ffb3f5 |
| work_keys_str_mv |
AT parkjh polydllacticglycolicacidndashstarglucosenanoparticlesforglucosetransporterandhypoglycemiamediatedtumortargeting AT chohj polydllacticglycolicacidndashstarglucosenanoparticlesforglucosetransporterandhypoglycemiamediatedtumortargeting AT kimdd polydllacticglycolicacidndashstarglucosenanoparticlesforglucosetransporterandhypoglycemiamediatedtumortargeting |
| _version_ |
1718401241420136448 |